Molecular identification of birds: performance of distance-based DNA barcoding in three genes to delimit parapatric species.

<h4>Background</h4>DNA barcoding based on the mitochondrial cytochrome oxidase subunit I gene (cox1 or COI) has been successful in species identification across a wide array of taxa but in some cases failed to delimit the species boundaries of closely allied allopatric species or of hybr...

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Autores principales:	Mansour Aliabadian, Mohammad Kaboli, Vincent Nijman, Miguel Vences
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/43ef3f20bc054e0b98ac54de05a69e5d
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spelling	oai:doaj.org-article:43ef3f20bc054e0b98ac54de05a69e5d2021-11-25T06:17:51ZMolecular identification of birds: performance of distance-based DNA barcoding in three genes to delimit parapatric species.1932-620310.1371/journal.pone.0004119https://doaj.org/article/43ef3f20bc054e0b98ac54de05a69e5d2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19127298/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>DNA barcoding based on the mitochondrial cytochrome oxidase subunit I gene (cox1 or COI) has been successful in species identification across a wide array of taxa but in some cases failed to delimit the species boundaries of closely allied allopatric species or of hybridising sister species.<h4>Methodology/principal findings</h4>In this study we extend the sample size of prior studies in birds for cox1 (2776 sequences, 756 species) and target especially species that are known to occur parapatrically, and/or are known to hybridise, on a Holarctic scale. In order to obtain a larger set of taxa (altogether 2719 species), we include also DNA sequences of two other mitochondrial genes: cytochrome b (cob) (4614 sequences, 2087 species) and 16S (708 sequences, 498 species). Our results confirm the existence of a wide gap between intra- and interspecies divergences for both cox1 and cob, and indicate that distance-based DNA barcoding provides sufficient information to identify and delineate bird species in 98% of all possible pairwise comparisons. This DNA barcoding gap was not statistically influenced by the number of individuals sequenced per species. However, most of the hybridising parapatric species pairs have average divergences intermediate between intraspecific and interspecific distances for both cox1 and cob.<h4>Conclusions/significance</h4>DNA barcoding, if used as a tool for species discovery, would thus fail to identify hybridising parapatric species pairs. However, most of them can probably still assigned to known species by character-based approaches, although development of complementary nuclear markers will be necessary to account for mitochondrial introgression in hybridising species.Mansour AliabadianMohammad KaboliVincent NijmanMiguel VencesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 1, p e4119 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mansour Aliabadian Mohammad Kaboli Vincent Nijman Miguel Vences Molecular identification of birds: performance of distance-based DNA barcoding in three genes to delimit parapatric species.
description	<h4>Background</h4>DNA barcoding based on the mitochondrial cytochrome oxidase subunit I gene (cox1 or COI) has been successful in species identification across a wide array of taxa but in some cases failed to delimit the species boundaries of closely allied allopatric species or of hybridising sister species.<h4>Methodology/principal findings</h4>In this study we extend the sample size of prior studies in birds for cox1 (2776 sequences, 756 species) and target especially species that are known to occur parapatrically, and/or are known to hybridise, on a Holarctic scale. In order to obtain a larger set of taxa (altogether 2719 species), we include also DNA sequences of two other mitochondrial genes: cytochrome b (cob) (4614 sequences, 2087 species) and 16S (708 sequences, 498 species). Our results confirm the existence of a wide gap between intra- and interspecies divergences for both cox1 and cob, and indicate that distance-based DNA barcoding provides sufficient information to identify and delineate bird species in 98% of all possible pairwise comparisons. This DNA barcoding gap was not statistically influenced by the number of individuals sequenced per species. However, most of the hybridising parapatric species pairs have average divergences intermediate between intraspecific and interspecific distances for both cox1 and cob.<h4>Conclusions/significance</h4>DNA barcoding, if used as a tool for species discovery, would thus fail to identify hybridising parapatric species pairs. However, most of them can probably still assigned to known species by character-based approaches, although development of complementary nuclear markers will be necessary to account for mitochondrial introgression in hybridising species.
format	article
author	Mansour Aliabadian Mohammad Kaboli Vincent Nijman Miguel Vences
author_facet	Mansour Aliabadian Mohammad Kaboli Vincent Nijman Miguel Vences
author_sort	Mansour Aliabadian
title	Molecular identification of birds: performance of distance-based DNA barcoding in three genes to delimit parapatric species.
title_short	Molecular identification of birds: performance of distance-based DNA barcoding in three genes to delimit parapatric species.
title_full	Molecular identification of birds: performance of distance-based DNA barcoding in three genes to delimit parapatric species.
title_fullStr	Molecular identification of birds: performance of distance-based DNA barcoding in three genes to delimit parapatric species.
title_full_unstemmed	Molecular identification of birds: performance of distance-based DNA barcoding in three genes to delimit parapatric species.
title_sort	molecular identification of birds: performance of distance-based dna barcoding in three genes to delimit parapatric species.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/43ef3f20bc054e0b98ac54de05a69e5d
work_keys_str_mv	AT mansouraliabadian molecularidentificationofbirdsperformanceofdistancebaseddnabarcodinginthreegenestodelimitparapatricspecies AT mohammadkaboli molecularidentificationofbirdsperformanceofdistancebaseddnabarcodinginthreegenestodelimitparapatricspecies AT vincentnijman molecularidentificationofbirdsperformanceofdistancebaseddnabarcodinginthreegenestodelimitparapatricspecies AT miguelvences molecularidentificationofbirdsperformanceofdistancebaseddnabarcodinginthreegenestodelimitparapatricspecies
_version_	1718413921371553792

Molecular identification of birds: performance of distance-based DNA barcoding in three genes to delimit parapatric species.

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