Peptide substrates for Rho-associated kinase 2 (Rho-kinase 2/ROCK2).

Peptide substrates sensitive for a certain protein kinase could be important for new-drug development and to understand the mechanism of diseases. Rho-associated kinase (Rho-kinase/ROCK) is a serine/threonine kinase, and plays an important part in cardiovascular disease, migration and invasion of tu...

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Autores principales:	Jeong-Hun Kang, Daisuke Asai, Akira Tsuchiya, Takeshi Mori, Takuro Niidome, Yoshiki Katayama
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/43f0dbc4b13144f7803a6986d93cbd81
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spelling	oai:doaj.org-article:43f0dbc4b13144f7803a6986d93cbd812021-11-18T06:49:15ZPeptide substrates for Rho-associated kinase 2 (Rho-kinase 2/ROCK2).1932-620310.1371/journal.pone.0022699https://doaj.org/article/43f0dbc4b13144f7803a6986d93cbd812011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21818369/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Peptide substrates sensitive for a certain protein kinase could be important for new-drug development and to understand the mechanism of diseases. Rho-associated kinase (Rho-kinase/ROCK) is a serine/threonine kinase, and plays an important part in cardiovascular disease, migration and invasion of tumor cells, and in neurological disorders. The purpose of this study was to find substrates with high affinity and sensitivity for ROCK2. We synthesized 136 peptide substrates from protein substrates for ROCK2 with different lengths and charged peptides. Incorporation of (32)P [counts per minute (CPM)] for each peptide substrate was determined by the radiolabel assay using [γ-(32)P]ATP. When the top five peptide substrates showing high CPMs (R4, R22, R133, R134, and R135) were phosphorylated by other enzymes (PKA, PKCα, and ERK1), R22, R133, and R135 displayed the highest CPM level for ROCK2 compared with other enzymes, whereas R4 and R134 showed similar CPM levels for ROCK2 and PKCα. We hypothesize that R22, R133, and R135 can be useful peptide substrates for ROCK2.Jeong-Hun KangDaisuke AsaiAkira TsuchiyaTakeshi MoriTakuro NiidomeYoshiki KatayamaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22699 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeong-Hun Kang Daisuke Asai Akira Tsuchiya Takeshi Mori Takuro Niidome Yoshiki Katayama Peptide substrates for Rho-associated kinase 2 (Rho-kinase 2/ROCK2).
description	Peptide substrates sensitive for a certain protein kinase could be important for new-drug development and to understand the mechanism of diseases. Rho-associated kinase (Rho-kinase/ROCK) is a serine/threonine kinase, and plays an important part in cardiovascular disease, migration and invasion of tumor cells, and in neurological disorders. The purpose of this study was to find substrates with high affinity and sensitivity for ROCK2. We synthesized 136 peptide substrates from protein substrates for ROCK2 with different lengths and charged peptides. Incorporation of (32)P [counts per minute (CPM)] for each peptide substrate was determined by the radiolabel assay using [γ-(32)P]ATP. When the top five peptide substrates showing high CPMs (R4, R22, R133, R134, and R135) were phosphorylated by other enzymes (PKA, PKCα, and ERK1), R22, R133, and R135 displayed the highest CPM level for ROCK2 compared with other enzymes, whereas R4 and R134 showed similar CPM levels for ROCK2 and PKCα. We hypothesize that R22, R133, and R135 can be useful peptide substrates for ROCK2.
format	article
author	Jeong-Hun Kang Daisuke Asai Akira Tsuchiya Takeshi Mori Takuro Niidome Yoshiki Katayama
author_facet	Jeong-Hun Kang Daisuke Asai Akira Tsuchiya Takeshi Mori Takuro Niidome Yoshiki Katayama
author_sort	Jeong-Hun Kang
title	Peptide substrates for Rho-associated kinase 2 (Rho-kinase 2/ROCK2).
title_short	Peptide substrates for Rho-associated kinase 2 (Rho-kinase 2/ROCK2).
title_full	Peptide substrates for Rho-associated kinase 2 (Rho-kinase 2/ROCK2).
title_fullStr	Peptide substrates for Rho-associated kinase 2 (Rho-kinase 2/ROCK2).
title_full_unstemmed	Peptide substrates for Rho-associated kinase 2 (Rho-kinase 2/ROCK2).
title_sort	peptide substrates for rho-associated kinase 2 (rho-kinase 2/rock2).
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/43f0dbc4b13144f7803a6986d93cbd81
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_version_	1718424347566145536

Peptide substrates for Rho-associated kinase 2 (Rho-kinase 2/ROCK2).

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