The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior

Abstract A weak alkaline environment is established to facilitate the growth of osteoblasts. Unfortunately, this is inconsistent with the application of biodegradable polymer in bone regeneration, as the degradation products are usually acidic. In this study, the variation of the interfacial pH of p...

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Autores principales:	Changshun Ruan, Nan Hu, Yufei Ma, Yuxiao Li, Juan Liu, Xinzhou Zhang, Haobo Pan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/43f198d4cc68402082bf70ac0f4f3868
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spelling	oai:doaj.org-article:43f198d4cc68402082bf70ac0f4f38682021-12-02T15:05:27ZThe interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior10.1038/s41598-017-06354-12045-2322https://doaj.org/article/43f198d4cc68402082bf70ac0f4f38682017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06354-1https://doaj.org/toc/2045-2322Abstract A weak alkaline environment is established to facilitate the growth of osteoblasts. Unfortunately, this is inconsistent with the application of biodegradable polymer in bone regeneration, as the degradation products are usually acidic. In this study, the variation of the interfacial pH of poly (D, L-lactide) and piperazine-based polyurethane ureas (P-PUUs), as the representations of acidic degradable materials, and the behavior of osteoblasts on these substrates with tunable interfacial pH were investigated in vitro. These results revealed that the release of degraded products caused a rapid decrease in the interfacial pH, and this could be relieved by the introduction of alkaline segments. On the contrary, when culturing with osteoblasts, the variation of the interfacial pH revealed an upward tendency, indicating that cell could construct the microenvironment by secreting cellular metabolites to satisfy its own survival. In addition, the behavior of osteoblasts on substrates exhibited that P-PUUs with the most PP units were better for cell growth and osteogenic differentiation of cells. This is due to the hydrophilic surface and the moderate N% in P-PUUs, key factors in the promotion of the early stages of cellular responses, and the interfacial pH contributing to the enhanced effect on osteogenic differentiation.Changshun RuanNan HuYufei MaYuxiao LiJuan LiuXinzhou ZhangHaobo PanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Changshun Ruan Nan Hu Yufei Ma Yuxiao Li Juan Liu Xinzhou Zhang Haobo Pan The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior
description	Abstract A weak alkaline environment is established to facilitate the growth of osteoblasts. Unfortunately, this is inconsistent with the application of biodegradable polymer in bone regeneration, as the degradation products are usually acidic. In this study, the variation of the interfacial pH of poly (D, L-lactide) and piperazine-based polyurethane ureas (P-PUUs), as the representations of acidic degradable materials, and the behavior of osteoblasts on these substrates with tunable interfacial pH were investigated in vitro. These results revealed that the release of degraded products caused a rapid decrease in the interfacial pH, and this could be relieved by the introduction of alkaline segments. On the contrary, when culturing with osteoblasts, the variation of the interfacial pH revealed an upward tendency, indicating that cell could construct the microenvironment by secreting cellular metabolites to satisfy its own survival. In addition, the behavior of osteoblasts on substrates exhibited that P-PUUs with the most PP units were better for cell growth and osteogenic differentiation of cells. This is due to the hydrophilic surface and the moderate N% in P-PUUs, key factors in the promotion of the early stages of cellular responses, and the interfacial pH contributing to the enhanced effect on osteogenic differentiation.
format	article
author	Changshun Ruan Nan Hu Yufei Ma Yuxiao Li Juan Liu Xinzhou Zhang Haobo Pan
author_facet	Changshun Ruan Nan Hu Yufei Ma Yuxiao Li Juan Liu Xinzhou Zhang Haobo Pan
author_sort	Changshun Ruan
title	The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior
title_short	The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior
title_full	The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior
title_fullStr	The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior
title_full_unstemmed	The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior
title_sort	interfacial ph of acidic degradable polymeric biomaterials and its effects on osteoblast behavior
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/43f198d4cc68402082bf70ac0f4f3868
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The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior

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