Cytotoxicity of selenium nanoparticles in rat dermal fibroblasts

Joseph F Ramos,1 Thomas J Webster21School of Engineering, Center of Biomedical Engineering, 2School of Engineering and Department of Orthopedics, Brown University, Providence, RI, USABackground: Ventilator-associated pneumonia is a deadly nosocomial infection caused by contaminated endotracheal tube...

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Autores principales:	Ramos JF, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/43f3e21b709f4bbc963de424154b7049
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spelling	oai:doaj.org-article:43f3e21b709f4bbc963de424154b70492021-12-02T05:14:28ZCytotoxicity of selenium nanoparticles in rat dermal fibroblasts1176-91141178-2013https://doaj.org/article/43f3e21b709f4bbc963de424154b70492012-07-01T00:00:00Zhttp://www.dovepress.com/cytotoxicity-of-selenium-nanoparticles-in-rat-dermal-fibroblasts-a10480https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Joseph F Ramos,1 Thomas J Webster21School of Engineering, Center of Biomedical Engineering, 2School of Engineering and Department of Orthopedics, Brown University, Providence, RI, USABackground: Ventilator-associated pneumonia is a deadly nosocomial infection caused by contaminated endotracheal tubes. It has been shown that polyvinyl chloride (PVC, the endotracheal tube substrate) coated with elemental selenium nanoparticles reduces bacterial adherence and proliferation on PVC by over 99%. However, it is not known if selenium nanoparticles elicit a cytotoxic effect in vitro. The purpose of this study was to investigate the cytotoxic effects of PVC coated with selenium nanoparticles on fibroblasts, which are mammalian cells central to endotracheal tube intubation.Methods: Different concentrations of selenium nanoparticles were precipitated onto the PVC surface by reduction of selenium salts using glutathione. Characterization of PVC coated with selenium nanoparticles was done by scanning electron microscopy, energy dispersive x-ray, and contact angle measurements. For the cytotoxicity experiments, fibroblasts were seeded at a density of 5000 cm2 onto PVC coated with three different concentrations of selenium nanoparticles (high, medium, low) and incubated for 4 hours (adhesion) as well as for 24 hours and 72 hours (proliferation). The half-maximal inhibitory concentration (IC50) value was determined after 72 hours using an ultrahigh concentration. MTT assays were used to assess cell viability at the indicated time points.Results: The three concentrations of selenium nanoparticles did not elicit a cytotoxic effect after 72 hours (P &lt; 0.01, n = 3). It was found that the IC50 value was at the ultrahigh concentration of selenium nanoparticles. The nanoparticulate elemental selenium concentration previously shown to decrease the function of bacteria was shown not to cause a cytotoxic effect on fibroblasts in vitro.Conclusion: These findings demonstrate great selectivity between bacteria and healthy cells, and are a viable option for coating endotracheal tubes in order to prevent ventilator-associated pneumonia.Keywords: selenium, nanoparticle, cytotoxicity, fibroblast, ventilator-associated pneumoniaRamos JFWebster TJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3907-3914 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ramos JF Webster TJ Cytotoxicity of selenium nanoparticles in rat dermal fibroblasts
description	Joseph F Ramos,1 Thomas J Webster21School of Engineering, Center of Biomedical Engineering, 2School of Engineering and Department of Orthopedics, Brown University, Providence, RI, USABackground: Ventilator-associated pneumonia is a deadly nosocomial infection caused by contaminated endotracheal tubes. It has been shown that polyvinyl chloride (PVC, the endotracheal tube substrate) coated with elemental selenium nanoparticles reduces bacterial adherence and proliferation on PVC by over 99%. However, it is not known if selenium nanoparticles elicit a cytotoxic effect in vitro. The purpose of this study was to investigate the cytotoxic effects of PVC coated with selenium nanoparticles on fibroblasts, which are mammalian cells central to endotracheal tube intubation.Methods: Different concentrations of selenium nanoparticles were precipitated onto the PVC surface by reduction of selenium salts using glutathione. Characterization of PVC coated with selenium nanoparticles was done by scanning electron microscopy, energy dispersive x-ray, and contact angle measurements. For the cytotoxicity experiments, fibroblasts were seeded at a density of 5000 cm2 onto PVC coated with three different concentrations of selenium nanoparticles (high, medium, low) and incubated for 4 hours (adhesion) as well as for 24 hours and 72 hours (proliferation). The half-maximal inhibitory concentration (IC50) value was determined after 72 hours using an ultrahigh concentration. MTT assays were used to assess cell viability at the indicated time points.Results: The three concentrations of selenium nanoparticles did not elicit a cytotoxic effect after 72 hours (P &lt; 0.01, n = 3). It was found that the IC50 value was at the ultrahigh concentration of selenium nanoparticles. The nanoparticulate elemental selenium concentration previously shown to decrease the function of bacteria was shown not to cause a cytotoxic effect on fibroblasts in vitro.Conclusion: These findings demonstrate great selectivity between bacteria and healthy cells, and are a viable option for coating endotracheal tubes in order to prevent ventilator-associated pneumonia.Keywords: selenium, nanoparticle, cytotoxicity, fibroblast, ventilator-associated pneumonia
format	article
author	Ramos JF Webster TJ
author_facet	Ramos JF Webster TJ
author_sort	Ramos JF
title	Cytotoxicity of selenium nanoparticles in rat dermal fibroblasts
title_short	Cytotoxicity of selenium nanoparticles in rat dermal fibroblasts
title_full	Cytotoxicity of selenium nanoparticles in rat dermal fibroblasts
title_fullStr	Cytotoxicity of selenium nanoparticles in rat dermal fibroblasts
title_full_unstemmed	Cytotoxicity of selenium nanoparticles in rat dermal fibroblasts
title_sort	cytotoxicity of selenium nanoparticles in rat dermal fibroblasts
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/43f3e21b709f4bbc963de424154b7049
work_keys_str_mv	AT ramosjf cytotoxicityofseleniumnanoparticlesinratdermalfibroblasts AT webstertj cytotoxicityofseleniumnanoparticlesinratdermalfibroblasts
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Cytotoxicity of selenium nanoparticles in rat dermal fibroblasts

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