Review of Recent Advances in Lipid Analysis of Biological Samples via Ambient Ionization Mass Spectrometry

The rapid and direct structural characterization of lipids proves to be critical for studying the functional roles of lipids in many biological processes. Among numerous analytical techniques, ambient ionization mass spectrometry (AIMS) allows for a direct molecular characterization of lipids from v...

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Autores principales:	Haiyan Lu, Hua Zhang, Shuling Xu, Lingjun Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lipidomics structural elucidation ambient ionization mass spectrometry biological samples Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/43f56b5ff3424beb9537376298768483
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spelling	oai:doaj.org-article:43f56b5ff3424beb95373762987684832021-11-25T18:20:53ZReview of Recent Advances in Lipid Analysis of Biological Samples via Ambient Ionization Mass Spectrometry10.3390/metabo111107812218-1989https://doaj.org/article/43f56b5ff3424beb95373762987684832021-11-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/781https://doaj.org/toc/2218-1989The rapid and direct structural characterization of lipids proves to be critical for studying the functional roles of lipids in many biological processes. Among numerous analytical techniques, ambient ionization mass spectrometry (AIMS) allows for a direct molecular characterization of lipids from various complex biological samples with no/minimal sample pretreatment. Over the recent years, researchers have expanded the applications of the AIMS techniques to lipid structural elucidation via a combination with a series of derivatization strategies (e.g., the Paternò–Büchi (PB) reaction, ozone-induced dissociation (OzID), and epoxidation reaction), including carbon–carbon double bond (C=C) locations and sn-positions isomers. Herein, this review summarizes the reaction mechanisms of various derivatization strategies for C=C bond analysis, typical instrumental setup, and applications of AIMS in the structural elucidation of lipids from various biological samples (e.g., tissues, cells, and biofluids). In addition, future directions of AIMS for lipid structural elucidation are discussed.Haiyan LuHua ZhangShuling XuLingjun LiMDPI AGarticlelipidomicsstructural elucidationambient ionization mass spectrometrybiological samplesMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 781, p 781 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipidomics structural elucidation ambient ionization mass spectrometry biological samples Microbiology QR1-502
spellingShingle	lipidomics structural elucidation ambient ionization mass spectrometry biological samples Microbiology QR1-502 Haiyan Lu Hua Zhang Shuling Xu Lingjun Li Review of Recent Advances in Lipid Analysis of Biological Samples via Ambient Ionization Mass Spectrometry
description	The rapid and direct structural characterization of lipids proves to be critical for studying the functional roles of lipids in many biological processes. Among numerous analytical techniques, ambient ionization mass spectrometry (AIMS) allows for a direct molecular characterization of lipids from various complex biological samples with no/minimal sample pretreatment. Over the recent years, researchers have expanded the applications of the AIMS techniques to lipid structural elucidation via a combination with a series of derivatization strategies (e.g., the Paternò–Büchi (PB) reaction, ozone-induced dissociation (OzID), and epoxidation reaction), including carbon–carbon double bond (C=C) locations and sn-positions isomers. Herein, this review summarizes the reaction mechanisms of various derivatization strategies for C=C bond analysis, typical instrumental setup, and applications of AIMS in the structural elucidation of lipids from various biological samples (e.g., tissues, cells, and biofluids). In addition, future directions of AIMS for lipid structural elucidation are discussed.
format	article
author	Haiyan Lu Hua Zhang Shuling Xu Lingjun Li
author_facet	Haiyan Lu Hua Zhang Shuling Xu Lingjun Li
author_sort	Haiyan Lu
title	Review of Recent Advances in Lipid Analysis of Biological Samples via Ambient Ionization Mass Spectrometry
title_short	Review of Recent Advances in Lipid Analysis of Biological Samples via Ambient Ionization Mass Spectrometry
title_full	Review of Recent Advances in Lipid Analysis of Biological Samples via Ambient Ionization Mass Spectrometry
title_fullStr	Review of Recent Advances in Lipid Analysis of Biological Samples via Ambient Ionization Mass Spectrometry
title_full_unstemmed	Review of Recent Advances in Lipid Analysis of Biological Samples via Ambient Ionization Mass Spectrometry
title_sort	review of recent advances in lipid analysis of biological samples via ambient ionization mass spectrometry
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/43f56b5ff3424beb9537376298768483
work_keys_str_mv	AT haiyanlu reviewofrecentadvancesinlipidanalysisofbiologicalsamplesviaambientionizationmassspectrometry AT huazhang reviewofrecentadvancesinlipidanalysisofbiologicalsamplesviaambientionizationmassspectrometry AT shulingxu reviewofrecentadvancesinlipidanalysisofbiologicalsamplesviaambientionizationmassspectrometry AT lingjunli reviewofrecentadvancesinlipidanalysisofbiologicalsamplesviaambientionizationmassspectrometry
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Review of Recent Advances in Lipid Analysis of Biological Samples via Ambient Ionization Mass Spectrometry

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