Pervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics

Connecting conformational dynamics and epistasis has so far been limited to a few proteins and a single fitness trait. Here, the authors provide evidence of positive epistasis on multiple catalytic traits in the evolution and dynamics of engineered cytochrome P450 monooxygenase, offering insights fo...

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Autores principales:	Carlos G. Acevedo-Rocha, Aitao Li, Lorenzo D’Amore, Sabrina Hoebenreich, Joaquin Sanchis, Paul Lubrano, Matteo P. Ferla, Marc Garcia-Borràs, Sílvia Osuna, Manfred T. Reetz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4402820afefc4a3885f8088826bfca5b
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spelling	oai:doaj.org-article:4402820afefc4a3885f8088826bfca5b2021-12-02T15:52:28ZPervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics10.1038/s41467-021-21833-w2041-1723https://doaj.org/article/4402820afefc4a3885f8088826bfca5b2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21833-whttps://doaj.org/toc/2041-1723Connecting conformational dynamics and epistasis has so far been limited to a few proteins and a single fitness trait. Here, the authors provide evidence of positive epistasis on multiple catalytic traits in the evolution and dynamics of engineered cytochrome P450 monooxygenase, offering insights for in silico protein design.Carlos G. Acevedo-RochaAitao LiLorenzo D’AmoreSabrina HoebenreichJoaquin SanchisPaul LubranoMatteo P. FerlaMarc Garcia-BorràsSílvia OsunaManfred T. ReetzNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Carlos G. Acevedo-Rocha Aitao Li Lorenzo D’Amore Sabrina Hoebenreich Joaquin Sanchis Paul Lubrano Matteo P. Ferla Marc Garcia-Borràs Sílvia Osuna Manfred T. Reetz Pervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics
description	Connecting conformational dynamics and epistasis has so far been limited to a few proteins and a single fitness trait. Here, the authors provide evidence of positive epistasis on multiple catalytic traits in the evolution and dynamics of engineered cytochrome P450 monooxygenase, offering insights for in silico protein design.
format	article
author	Carlos G. Acevedo-Rocha Aitao Li Lorenzo D’Amore Sabrina Hoebenreich Joaquin Sanchis Paul Lubrano Matteo P. Ferla Marc Garcia-Borràs Sílvia Osuna Manfred T. Reetz
author_facet	Carlos G. Acevedo-Rocha Aitao Li Lorenzo D’Amore Sabrina Hoebenreich Joaquin Sanchis Paul Lubrano Matteo P. Ferla Marc Garcia-Borràs Sílvia Osuna Manfred T. Reetz
author_sort	Carlos G. Acevedo-Rocha
title	Pervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics
title_short	Pervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics
title_full	Pervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics
title_fullStr	Pervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics
title_full_unstemmed	Pervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics
title_sort	pervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4402820afefc4a3885f8088826bfca5b
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Pervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics

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