Potential role of organic matter in the transmission of antibiotic resistance genes in black soils

The degradation of black soil is a serious problem with the decrease in soil organic matter (SOM) content in northeast China, and animal manure as a reservoir of antibiotic resistance genes (ARGs) is commonly amended into soil to sustain or increase the SOM content. However, the potential effect of...

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Autores principales:	Sen Li, Junjie Liu, Qin Yao, Zhenhua Yu, Yansheng Li, Jian Jin, Xiaobing Liu, Guanghua Wang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Mollisols Soil resistome Soil bacterial community HT-PCR Illumina MiSeq Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/4406d50522b4491abfa94b75841a5e7c
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spelling	oai:doaj.org-article:4406d50522b4491abfa94b75841a5e7c2021-11-06T04:19:04ZPotential role of organic matter in the transmission of antibiotic resistance genes in black soils0147-651310.1016/j.ecoenv.2021.112946https://doaj.org/article/4406d50522b4491abfa94b75841a5e7c2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010587https://doaj.org/toc/0147-6513The degradation of black soil is a serious problem with the decrease in soil organic matter (SOM) content in northeast China, and animal manure as a reservoir of antibiotic resistance genes (ARGs) is commonly amended into soil to sustain or increase the SOM content. However, the potential effect of SOM content on soil resistome remains unclear. Here, a soil microcosm experiment was established to explore the temporal succession of antibiotic resistance genes (ARGs) and bacterial communities in three black soils with distinct difference in SOM contents following application of poultry manure using high-throughput qPCR (HT-qPCR) and MiSeq sequencing. A total of 151 ARGs and 8 mobile genetic elements (MGEs) were detected across all samples. Relative abundance of ARGs negatively correlated with SOM content. Manure-derived ARGs had much higher diversity and absolute abundance in the low SOM soils. The ARG composition and bacterial community structure were significantly different in three soils. A random forest model showed that SOM content was a better predictor of ARG pattern than bacterial diversity and abundance. Structural equation modeling indicated that the negative effects of SOM content on ARG patterns was accomplished by the shift of bacterial communities such as the bacterial diversity and abundance. Our study demonstrated that SOM content could play an important role in the dissemination of ARGs originated from animal manures, these findings provide a possible strategy for the suppression of the spread of ARGs in black soils by increasing SOM content.Sen LiJunjie LiuQin YaoZhenhua YuYansheng LiJian JinXiaobing LiuGuanghua WangElsevierarticleMollisolsSoil resistomeSoil bacterial communityHT-PCRIllumina MiSeqEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112946- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mollisols Soil resistome Soil bacterial community HT-PCR Illumina MiSeq Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Mollisols Soil resistome Soil bacterial community HT-PCR Illumina MiSeq Environmental pollution TD172-193.5 Environmental sciences GE1-350 Sen Li Junjie Liu Qin Yao Zhenhua Yu Yansheng Li Jian Jin Xiaobing Liu Guanghua Wang Potential role of organic matter in the transmission of antibiotic resistance genes in black soils
description	The degradation of black soil is a serious problem with the decrease in soil organic matter (SOM) content in northeast China, and animal manure as a reservoir of antibiotic resistance genes (ARGs) is commonly amended into soil to sustain or increase the SOM content. However, the potential effect of SOM content on soil resistome remains unclear. Here, a soil microcosm experiment was established to explore the temporal succession of antibiotic resistance genes (ARGs) and bacterial communities in three black soils with distinct difference in SOM contents following application of poultry manure using high-throughput qPCR (HT-qPCR) and MiSeq sequencing. A total of 151 ARGs and 8 mobile genetic elements (MGEs) were detected across all samples. Relative abundance of ARGs negatively correlated with SOM content. Manure-derived ARGs had much higher diversity and absolute abundance in the low SOM soils. The ARG composition and bacterial community structure were significantly different in three soils. A random forest model showed that SOM content was a better predictor of ARG pattern than bacterial diversity and abundance. Structural equation modeling indicated that the negative effects of SOM content on ARG patterns was accomplished by the shift of bacterial communities such as the bacterial diversity and abundance. Our study demonstrated that SOM content could play an important role in the dissemination of ARGs originated from animal manures, these findings provide a possible strategy for the suppression of the spread of ARGs in black soils by increasing SOM content.
format	article
author	Sen Li Junjie Liu Qin Yao Zhenhua Yu Yansheng Li Jian Jin Xiaobing Liu Guanghua Wang
author_facet	Sen Li Junjie Liu Qin Yao Zhenhua Yu Yansheng Li Jian Jin Xiaobing Liu Guanghua Wang
author_sort	Sen Li
title	Potential role of organic matter in the transmission of antibiotic resistance genes in black soils
title_short	Potential role of organic matter in the transmission of antibiotic resistance genes in black soils
title_full	Potential role of organic matter in the transmission of antibiotic resistance genes in black soils
title_fullStr	Potential role of organic matter in the transmission of antibiotic resistance genes in black soils
title_full_unstemmed	Potential role of organic matter in the transmission of antibiotic resistance genes in black soils
title_sort	potential role of organic matter in the transmission of antibiotic resistance genes in black soils
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/4406d50522b4491abfa94b75841a5e7c
work_keys_str_mv	AT senli potentialroleoforganicmatterinthetransmissionofantibioticresistancegenesinblacksoils AT junjieliu potentialroleoforganicmatterinthetransmissionofantibioticresistancegenesinblacksoils AT qinyao potentialroleoforganicmatterinthetransmissionofantibioticresistancegenesinblacksoils AT zhenhuayu potentialroleoforganicmatterinthetransmissionofantibioticresistancegenesinblacksoils AT yanshengli potentialroleoforganicmatterinthetransmissionofantibioticresistancegenesinblacksoils AT jianjin potentialroleoforganicmatterinthetransmissionofantibioticresistancegenesinblacksoils AT xiaobingliu potentialroleoforganicmatterinthetransmissionofantibioticresistancegenesinblacksoils AT guanghuawang potentialroleoforganicmatterinthetransmissionofantibioticresistancegenesinblacksoils
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Potential role of organic matter in the transmission of antibiotic resistance genes in black soils

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