The Structure of an Injectisome Export Gate Demonstrates Conservation of Architecture in the Core Export Gate between Flagellar and Virulence Type III Secretion Systems

ABSTRACT Export of proteins through type III secretion systems (T3SS) is critical for motility and virulence of many major bacterial pathogens. Proteins are exported through a genetically defined export gate complex consisting of three proteins. We have recently shown at 4.2 Å that the flagellar com...

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Autores principales:	Steven Johnson, Lucas Kuhlen, Justin C. Deme, Patrizia Abrusci, Susan M. Lea
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	T3SS cryo-EM protein secretion virulence determinants Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/440945dcd4ea43eaa5c20d25e64a9515
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spelling	oai:doaj.org-article:440945dcd4ea43eaa5c20d25e64a95152021-11-15T15:55:23ZThe Structure of an Injectisome Export Gate Demonstrates Conservation of Architecture in the Core Export Gate between Flagellar and Virulence Type III Secretion Systems10.1128/mBio.00818-192150-7511https://doaj.org/article/440945dcd4ea43eaa5c20d25e64a95152019-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00818-19https://doaj.org/toc/2150-7511ABSTRACT Export of proteins through type III secretion systems (T3SS) is critical for motility and virulence of many major bacterial pathogens. Proteins are exported through a genetically defined export gate complex consisting of three proteins. We have recently shown at 4.2 Å that the flagellar complex of these three putative membrane proteins (FliPQR in flagellar systems, SctRST in virulence systems) assembles into an extramembrane helical assembly that likely seeds correct assembly of the rod. Here we present the structure of an equivalent complex from the Shigella virulence system at 3.5 Å by cryo-electron microscopy. This higher-resolution structure yields a more precise description of the structure and confirms the prediction of structural conservation in this core complex. Analysis of particle heterogeneity also suggests how the SctS/FliQ subunits sequentially assemble in the complex. IMPORTANCE Although predicted on the basis of sequence conservation, the work presented here formally demonstrates that all classes of type III secretion systems, flagellar or virulence, share the same architecture at the level of the core structures. This absolute conservation of the unusual extramembrane structure of the core export gate complex now allows work to move to focusing on both mechanistic studies of type III but also on fundamental studies of how such a complex is assembled.Steven JohnsonLucas KuhlenJustin C. DemePatrizia AbrusciSusan M. LeaAmerican Society for MicrobiologyarticleT3SScryo-EMprotein secretionvirulence determinantsMicrobiologyQR1-502ENmBio, Vol 10, Iss 3 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	T3SS cryo-EM protein secretion virulence determinants Microbiology QR1-502
spellingShingle	T3SS cryo-EM protein secretion virulence determinants Microbiology QR1-502 Steven Johnson Lucas Kuhlen Justin C. Deme Patrizia Abrusci Susan M. Lea The Structure of an Injectisome Export Gate Demonstrates Conservation of Architecture in the Core Export Gate between Flagellar and Virulence Type III Secretion Systems
description	ABSTRACT Export of proteins through type III secretion systems (T3SS) is critical for motility and virulence of many major bacterial pathogens. Proteins are exported through a genetically defined export gate complex consisting of three proteins. We have recently shown at 4.2 Å that the flagellar complex of these three putative membrane proteins (FliPQR in flagellar systems, SctRST in virulence systems) assembles into an extramembrane helical assembly that likely seeds correct assembly of the rod. Here we present the structure of an equivalent complex from the Shigella virulence system at 3.5 Å by cryo-electron microscopy. This higher-resolution structure yields a more precise description of the structure and confirms the prediction of structural conservation in this core complex. Analysis of particle heterogeneity also suggests how the SctS/FliQ subunits sequentially assemble in the complex. IMPORTANCE Although predicted on the basis of sequence conservation, the work presented here formally demonstrates that all classes of type III secretion systems, flagellar or virulence, share the same architecture at the level of the core structures. This absolute conservation of the unusual extramembrane structure of the core export gate complex now allows work to move to focusing on both mechanistic studies of type III but also on fundamental studies of how such a complex is assembled.
format	article
author	Steven Johnson Lucas Kuhlen Justin C. Deme Patrizia Abrusci Susan M. Lea
author_facet	Steven Johnson Lucas Kuhlen Justin C. Deme Patrizia Abrusci Susan M. Lea
author_sort	Steven Johnson
title	The Structure of an Injectisome Export Gate Demonstrates Conservation of Architecture in the Core Export Gate between Flagellar and Virulence Type III Secretion Systems
title_short	The Structure of an Injectisome Export Gate Demonstrates Conservation of Architecture in the Core Export Gate between Flagellar and Virulence Type III Secretion Systems
title_full	The Structure of an Injectisome Export Gate Demonstrates Conservation of Architecture in the Core Export Gate between Flagellar and Virulence Type III Secretion Systems
title_fullStr	The Structure of an Injectisome Export Gate Demonstrates Conservation of Architecture in the Core Export Gate between Flagellar and Virulence Type III Secretion Systems
title_full_unstemmed	The Structure of an Injectisome Export Gate Demonstrates Conservation of Architecture in the Core Export Gate between Flagellar and Virulence Type III Secretion Systems
title_sort	structure of an injectisome export gate demonstrates conservation of architecture in the core export gate between flagellar and virulence type iii secretion systems
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/440945dcd4ea43eaa5c20d25e64a9515
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The Structure of an Injectisome Export Gate Demonstrates Conservation of Architecture in the Core Export Gate between Flagellar and Virulence Type III Secretion Systems

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