Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse
Abstract Traumatic injury or surgical excision of diseased bone tissue usually require the reconstruction of large bone defects unable to heal spontaneously, especially in older individuals. This is a big challenge requiring the development of biomaterials mimicking the bone structure and capable of...
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Nature Portfolio
2017
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oai:doaj.org-article:4418df1642d049ec91d58dffe3cbc75f2021-12-02T12:32:54ZHuman adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse10.1038/s41598-017-07672-02045-2322https://doaj.org/article/4418df1642d049ec91d58dffe3cbc75f2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07672-0https://doaj.org/toc/2045-2322Abstract Traumatic injury or surgical excision of diseased bone tissue usually require the reconstruction of large bone defects unable to heal spontaneously, especially in older individuals. This is a big challenge requiring the development of biomaterials mimicking the bone structure and capable of inducing the right commitment of cells seeded within the scaffold. In particular, given their properties and large availability, the human adipose-derived stem cells are considered as the better candidate for autologous cell transplantation. In order to evaluate the regenerative potential of these cells along with an osteoinductive biomaterial, we have used collagen/hydroxyapatite scaffolds to test ectopic bone formation after subcutaneous implantation in mice. The process was analysed both in vivo, by Fluorescent Molecular Tomography (FMT), and ex vivo, to evaluate the formation of bone and vascular structures. The results have shown that the biomaterial could itself be able of promoting differentiation of host cells and bone formation, probably by means of its intrinsic chemical and structural properties, namely the microenvironment. However, when charged with human mesenchymal stem cells, the ectopic bone formation within the scaffold was increased. We believe that these results represent an important advancement in the field of bone physiology, as well as in regenerative medicine.Giovanna CalabreseRaffaella GiuffridaStefano ForteClaudia FabbiElisa FigalloLucia SalvatorelliLorenzo MemeoRosalba ParentiMassimo GulisanoRosario GulinoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
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Medicine R Science Q Giovanna Calabrese Raffaella Giuffrida Stefano Forte Claudia Fabbi Elisa Figallo Lucia Salvatorelli Lorenzo Memeo Rosalba Parenti Massimo Gulisano Rosario Gulino Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse |
| description |
Abstract Traumatic injury or surgical excision of diseased bone tissue usually require the reconstruction of large bone defects unable to heal spontaneously, especially in older individuals. This is a big challenge requiring the development of biomaterials mimicking the bone structure and capable of inducing the right commitment of cells seeded within the scaffold. In particular, given their properties and large availability, the human adipose-derived stem cells are considered as the better candidate for autologous cell transplantation. In order to evaluate the regenerative potential of these cells along with an osteoinductive biomaterial, we have used collagen/hydroxyapatite scaffolds to test ectopic bone formation after subcutaneous implantation in mice. The process was analysed both in vivo, by Fluorescent Molecular Tomography (FMT), and ex vivo, to evaluate the formation of bone and vascular structures. The results have shown that the biomaterial could itself be able of promoting differentiation of host cells and bone formation, probably by means of its intrinsic chemical and structural properties, namely the microenvironment. However, when charged with human mesenchymal stem cells, the ectopic bone formation within the scaffold was increased. We believe that these results represent an important advancement in the field of bone physiology, as well as in regenerative medicine. |
| format |
article |
| author |
Giovanna Calabrese Raffaella Giuffrida Stefano Forte Claudia Fabbi Elisa Figallo Lucia Salvatorelli Lorenzo Memeo Rosalba Parenti Massimo Gulisano Rosario Gulino |
| author_facet |
Giovanna Calabrese Raffaella Giuffrida Stefano Forte Claudia Fabbi Elisa Figallo Lucia Salvatorelli Lorenzo Memeo Rosalba Parenti Massimo Gulisano Rosario Gulino |
| author_sort |
Giovanna Calabrese |
| title |
Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse |
| title_short |
Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse |
| title_full |
Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse |
| title_fullStr |
Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse |
| title_full_unstemmed |
Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse |
| title_sort |
human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/4418df1642d049ec91d58dffe3cbc75f |
| work_keys_str_mv |
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