Identification of candidate PAX2-regulated genes implicated in human kidney development
Abstract PAX2 is a transcription factor essential for kidney development and the main causative gene for renal coloboma syndrome (RCS). The mechanisms of PAX2 action during kidney development have been evaluated in mice but not in humans. This is a critical gap in knowledge since important differenc...
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Nature Portfolio
2021
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oai:doaj.org-article:441f6fd5b00847cfaaed60ac37342f8f2021-12-02T16:55:54ZIdentification of candidate PAX2-regulated genes implicated in human kidney development10.1038/s41598-021-88743-12045-2322https://doaj.org/article/441f6fd5b00847cfaaed60ac37342f8f2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88743-1https://doaj.org/toc/2045-2322Abstract PAX2 is a transcription factor essential for kidney development and the main causative gene for renal coloboma syndrome (RCS). The mechanisms of PAX2 action during kidney development have been evaluated in mice but not in humans. This is a critical gap in knowledge since important differences have been reported in kidney development in the two species. In the present study, we hypothesized that key human PAX2-dependent kidney development genes are differentially expressed in nephron progenitor cells from induced pluripotent stem cells (iPSCs) in patients with RCS relative to healthy individuals. Cap analysis of gene expression revealed 189 candidate promoters and 71 candidate enhancers that were differentially activated by PAX2 in this system in three patients with RCS with PAX2 mutations. By comparing this list with the list of candidate Pax2-regulated mouse kidney development genes obtained from the Functional Annotation of the Mouse/Mammalian (FANTOM) database, we prioritized 17 genes. Furthermore, we ranked three genes—PBX1, POSTN, and ITGA9—as the top candidates based on closely aligned expression kinetics with PAX2 in the iPSC culture system and susceptibility to suppression by a Pax2 inhibitor in cultured mouse embryonic kidney explants. Identification of these genes may provide important information to clarify the pathogenesis of RCS, human kidney development, and kidney regeneration.Yuta YamamuraKengo FuruichiYasuhiro MurakawaShigeki HirabayashiMasahito YoshiharaKeisuke SakoShinji KitajimaTadashi ToyamaYasunori IwataNorihiko SakaiKazuyoshi HosomichiPhilip M. MurphyAtsushi TajimaKeisuke OkitaKenji OsafuneShuichi KanekoTakashi WadaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
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Medicine R Science Q Yuta Yamamura Kengo Furuichi Yasuhiro Murakawa Shigeki Hirabayashi Masahito Yoshihara Keisuke Sako Shinji Kitajima Tadashi Toyama Yasunori Iwata Norihiko Sakai Kazuyoshi Hosomichi Philip M. Murphy Atsushi Tajima Keisuke Okita Kenji Osafune Shuichi Kaneko Takashi Wada Identification of candidate PAX2-regulated genes implicated in human kidney development |
| description |
Abstract PAX2 is a transcription factor essential for kidney development and the main causative gene for renal coloboma syndrome (RCS). The mechanisms of PAX2 action during kidney development have been evaluated in mice but not in humans. This is a critical gap in knowledge since important differences have been reported in kidney development in the two species. In the present study, we hypothesized that key human PAX2-dependent kidney development genes are differentially expressed in nephron progenitor cells from induced pluripotent stem cells (iPSCs) in patients with RCS relative to healthy individuals. Cap analysis of gene expression revealed 189 candidate promoters and 71 candidate enhancers that were differentially activated by PAX2 in this system in three patients with RCS with PAX2 mutations. By comparing this list with the list of candidate Pax2-regulated mouse kidney development genes obtained from the Functional Annotation of the Mouse/Mammalian (FANTOM) database, we prioritized 17 genes. Furthermore, we ranked three genes—PBX1, POSTN, and ITGA9—as the top candidates based on closely aligned expression kinetics with PAX2 in the iPSC culture system and susceptibility to suppression by a Pax2 inhibitor in cultured mouse embryonic kidney explants. Identification of these genes may provide important information to clarify the pathogenesis of RCS, human kidney development, and kidney regeneration. |
| format |
article |
| author |
Yuta Yamamura Kengo Furuichi Yasuhiro Murakawa Shigeki Hirabayashi Masahito Yoshihara Keisuke Sako Shinji Kitajima Tadashi Toyama Yasunori Iwata Norihiko Sakai Kazuyoshi Hosomichi Philip M. Murphy Atsushi Tajima Keisuke Okita Kenji Osafune Shuichi Kaneko Takashi Wada |
| author_facet |
Yuta Yamamura Kengo Furuichi Yasuhiro Murakawa Shigeki Hirabayashi Masahito Yoshihara Keisuke Sako Shinji Kitajima Tadashi Toyama Yasunori Iwata Norihiko Sakai Kazuyoshi Hosomichi Philip M. Murphy Atsushi Tajima Keisuke Okita Kenji Osafune Shuichi Kaneko Takashi Wada |
| author_sort |
Yuta Yamamura |
| title |
Identification of candidate PAX2-regulated genes implicated in human kidney development |
| title_short |
Identification of candidate PAX2-regulated genes implicated in human kidney development |
| title_full |
Identification of candidate PAX2-regulated genes implicated in human kidney development |
| title_fullStr |
Identification of candidate PAX2-regulated genes implicated in human kidney development |
| title_full_unstemmed |
Identification of candidate PAX2-regulated genes implicated in human kidney development |
| title_sort |
identification of candidate pax2-regulated genes implicated in human kidney development |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/441f6fd5b00847cfaaed60ac37342f8f |
| work_keys_str_mv |
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