Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps

Whole-cell catalyst-based hydroxytyrosol production is low. Here, the authors increase the efficiency of its production in E. coli by de-bottlenecking two enzymatic steps catalyzed by monooxygenase and tyramine oxidase using structure-based enzyme redesign or in vivo-directed evolution with the aid...

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Autores principales:	Jun Yao, Yang He, Nannan Su, Sakshibeedu R. Bharath, Yong Tao, Jian-Ming Jin, Wei Chen, Haiwei Song, Shuang-Yan Tang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4421dbb40aff41e8839dc00144021092
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spelling	oai:doaj.org-article:4421dbb40aff41e8839dc001440210922021-12-02T15:37:20ZDeveloping a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps10.1038/s41467-020-14918-52041-1723https://doaj.org/article/4421dbb40aff41e8839dc001440210922020-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14918-5https://doaj.org/toc/2041-1723Whole-cell catalyst-based hydroxytyrosol production is low. Here, the authors increase the efficiency of its production in E. coli by de-bottlenecking two enzymatic steps catalyzed by monooxygenase and tyramine oxidase using structure-based enzyme redesign or in vivo-directed evolution with the aid of a newly developed biosensor.Jun YaoYang HeNannan SuSakshibeedu R. BharathYong TaoJian-Ming JinWei ChenHaiwei SongShuang-Yan TangNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jun Yao Yang He Nannan Su Sakshibeedu R. Bharath Yong Tao Jian-Ming Jin Wei Chen Haiwei Song Shuang-Yan Tang Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps
description	Whole-cell catalyst-based hydroxytyrosol production is low. Here, the authors increase the efficiency of its production in E. coli by de-bottlenecking two enzymatic steps catalyzed by monooxygenase and tyramine oxidase using structure-based enzyme redesign or in vivo-directed evolution with the aid of a newly developed biosensor.
format	article
author	Jun Yao Yang He Nannan Su Sakshibeedu R. Bharath Yong Tao Jian-Ming Jin Wei Chen Haiwei Song Shuang-Yan Tang
author_facet	Jun Yao Yang He Nannan Su Sakshibeedu R. Bharath Yong Tao Jian-Ming Jin Wei Chen Haiwei Song Shuang-Yan Tang
author_sort	Jun Yao
title	Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps
title_short	Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps
title_full	Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps
title_fullStr	Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps
title_full_unstemmed	Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps
title_sort	developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4421dbb40aff41e8839dc00144021092
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Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps

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