Long noncoding RNA expression profile changes associated with dietary energy in the sheep testis during sexual maturation

Abstract Spermatogenesis can be affected by nutrition, which operates through normal physiological processes by changing the testicular mass and hormone levels profoundly. However, little is known regarding how testis development is regulated by long noncoding RNA (lncRNA). In this study, we investi...

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Autores principales:	Yanli Zhang, Hua Yang, Le Han, Fengzhe Li, Tingting Zhang, Jing Pang, Xu Feng, Caifang Ren, Shengyong Mao, Feng Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/442422a3e5c545d5aa46230f4234daa8
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spelling	oai:doaj.org-article:442422a3e5c545d5aa46230f4234daa82021-12-02T16:06:30ZLong noncoding RNA expression profile changes associated with dietary energy in the sheep testis during sexual maturation10.1038/s41598-017-05443-52045-2322https://doaj.org/article/442422a3e5c545d5aa46230f4234daa82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05443-5https://doaj.org/toc/2045-2322Abstract Spermatogenesis can be affected by nutrition, which operates through normal physiological processes by changing the testicular mass and hormone levels profoundly. However, little is known regarding how testis development is regulated by long noncoding RNA (lncRNA). In this study, we investigated the effects of high-grain (HG) feeding on testis development during sexual maturation mediated by lncRNA. The HG diet group showed an increase in growth hormone (GH), insulin-like growth factor-1 (IGF-1) and testosterone (T) levels, and in the number of sperm in the seminiferous tubules compared with the hay-fed group (p < 0.05). Moreover, we found 59 differentially expressed (DE) lncRNAs and 229 DE mRNAs in sheep testis between the two groups. qRT-PCR results of 20 randomly selected DE lncRNAs and mRNAs were also consistent with the RNA-seq data. Through functional enrichment analysis and lncRNA-mRNA interaction network analysis, we screened several lncRNAs that may be enriched for male reproduction such as spermatogenesis, sperm motility, steroid hormones, MAPK and ErbB signaling pathways. This study provides a first insight into the development of the testis with HG feeding in sheep and shows that these changes are associated with alterations in lncRNA expression.Yanli ZhangHua YangLe HanFengzhe LiTingting ZhangJing PangXu FengCaifang RenShengyong MaoFeng WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanli Zhang Hua Yang Le Han Fengzhe Li Tingting Zhang Jing Pang Xu Feng Caifang Ren Shengyong Mao Feng Wang Long noncoding RNA expression profile changes associated with dietary energy in the sheep testis during sexual maturation
description	Abstract Spermatogenesis can be affected by nutrition, which operates through normal physiological processes by changing the testicular mass and hormone levels profoundly. However, little is known regarding how testis development is regulated by long noncoding RNA (lncRNA). In this study, we investigated the effects of high-grain (HG) feeding on testis development during sexual maturation mediated by lncRNA. The HG diet group showed an increase in growth hormone (GH), insulin-like growth factor-1 (IGF-1) and testosterone (T) levels, and in the number of sperm in the seminiferous tubules compared with the hay-fed group (p < 0.05). Moreover, we found 59 differentially expressed (DE) lncRNAs and 229 DE mRNAs in sheep testis between the two groups. qRT-PCR results of 20 randomly selected DE lncRNAs and mRNAs were also consistent with the RNA-seq data. Through functional enrichment analysis and lncRNA-mRNA interaction network analysis, we screened several lncRNAs that may be enriched for male reproduction such as spermatogenesis, sperm motility, steroid hormones, MAPK and ErbB signaling pathways. This study provides a first insight into the development of the testis with HG feeding in sheep and shows that these changes are associated with alterations in lncRNA expression.
format	article
author	Yanli Zhang Hua Yang Le Han Fengzhe Li Tingting Zhang Jing Pang Xu Feng Caifang Ren Shengyong Mao Feng Wang
author_facet	Yanli Zhang Hua Yang Le Han Fengzhe Li Tingting Zhang Jing Pang Xu Feng Caifang Ren Shengyong Mao Feng Wang
author_sort	Yanli Zhang
title	Long noncoding RNA expression profile changes associated with dietary energy in the sheep testis during sexual maturation
title_short	Long noncoding RNA expression profile changes associated with dietary energy in the sheep testis during sexual maturation
title_full	Long noncoding RNA expression profile changes associated with dietary energy in the sheep testis during sexual maturation
title_fullStr	Long noncoding RNA expression profile changes associated with dietary energy in the sheep testis during sexual maturation
title_full_unstemmed	Long noncoding RNA expression profile changes associated with dietary energy in the sheep testis during sexual maturation
title_sort	long noncoding rna expression profile changes associated with dietary energy in the sheep testis during sexual maturation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/442422a3e5c545d5aa46230f4234daa8
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Long noncoding RNA expression profile changes associated with dietary energy in the sheep testis during sexual maturation

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