Genus-Wide Characterization of Nuclear Mitochondrial DNAs in Bumblebee (Hymenoptera: Apidae) Genomes

In eukaryotes, DNA of mitochondria is transferred into the nucleus and forms nuclear mitochondrial DNAs (NUMTs). Taking advantage of the abundant genomic resources for bumblebees, in this study, we de novo generated mitochondrial genomes (mitogenomes) for 11 bumblebee species. Then, we identified an...

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Autores principales:	Lele Ding, Huiling Sang, Cheng Sun
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bumblebee mitogenome NUMT genome innovation Science Q
Acceso en línea:	https://doaj.org/article/4437370c533845c78111d4ca18a1bc7e
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spelling	oai:doaj.org-article:4437370c533845c78111d4ca18a1bc7e2021-11-25T17:59:06ZGenus-Wide Characterization of Nuclear Mitochondrial DNAs in Bumblebee (Hymenoptera: Apidae) Genomes10.3390/insects121109632075-4450https://doaj.org/article/4437370c533845c78111d4ca18a1bc7e2021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4450/12/11/963https://doaj.org/toc/2075-4450In eukaryotes, DNA of mitochondria is transferred into the nucleus and forms nuclear mitochondrial DNAs (NUMTs). Taking advantage of the abundant genomic resources for bumblebees, in this study, we de novo generated mitochondrial genomes (mitogenomes) for 11 bumblebee species. Then, we identified and characterized NUMTs in genus-wide bumblebee species. The number of identified NUMTs varies across those species, with numbers ranging from 32 to 72, and nuclear genome size is not positively related to NUMT number. The insertion sites of NUMTs in the nuclear genome are not random, with AT-rich regions harboring more NUMTs. In addition, our results suggest that NUMTs derived from the mitochondrial COX1 gene are most abundant in the bumblebee nuclear genome. Although the majority of NUMTs are found within intergenic regions, some NUMTs do reside within genic regions. Transcripts that contain both the NUMT sequence and its flanking non-NUMT sequences could be found in the bumblebee transcriptome, suggesting a potential domestication of NUMTs in the bumblebee. Taken together, our results shed light on the molecular features of NUMTs in the bumblebee and uncover their contribution to genome innovation.Lele DingHuiling SangCheng SunMDPI AGarticlebumblebeemitogenomeNUMTgenome innovationScienceQENInsects, Vol 12, Iss 963, p 963 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bumblebee mitogenome NUMT genome innovation Science Q
spellingShingle	bumblebee mitogenome NUMT genome innovation Science Q Lele Ding Huiling Sang Cheng Sun Genus-Wide Characterization of Nuclear Mitochondrial DNAs in Bumblebee (Hymenoptera: Apidae) Genomes
description	In eukaryotes, DNA of mitochondria is transferred into the nucleus and forms nuclear mitochondrial DNAs (NUMTs). Taking advantage of the abundant genomic resources for bumblebees, in this study, we de novo generated mitochondrial genomes (mitogenomes) for 11 bumblebee species. Then, we identified and characterized NUMTs in genus-wide bumblebee species. The number of identified NUMTs varies across those species, with numbers ranging from 32 to 72, and nuclear genome size is not positively related to NUMT number. The insertion sites of NUMTs in the nuclear genome are not random, with AT-rich regions harboring more NUMTs. In addition, our results suggest that NUMTs derived from the mitochondrial COX1 gene are most abundant in the bumblebee nuclear genome. Although the majority of NUMTs are found within intergenic regions, some NUMTs do reside within genic regions. Transcripts that contain both the NUMT sequence and its flanking non-NUMT sequences could be found in the bumblebee transcriptome, suggesting a potential domestication of NUMTs in the bumblebee. Taken together, our results shed light on the molecular features of NUMTs in the bumblebee and uncover their contribution to genome innovation.
format	article
author	Lele Ding Huiling Sang Cheng Sun
author_facet	Lele Ding Huiling Sang Cheng Sun
author_sort	Lele Ding
title	Genus-Wide Characterization of Nuclear Mitochondrial DNAs in Bumblebee (Hymenoptera: Apidae) Genomes
title_short	Genus-Wide Characterization of Nuclear Mitochondrial DNAs in Bumblebee (Hymenoptera: Apidae) Genomes
title_full	Genus-Wide Characterization of Nuclear Mitochondrial DNAs in Bumblebee (Hymenoptera: Apidae) Genomes
title_fullStr	Genus-Wide Characterization of Nuclear Mitochondrial DNAs in Bumblebee (Hymenoptera: Apidae) Genomes
title_full_unstemmed	Genus-Wide Characterization of Nuclear Mitochondrial DNAs in Bumblebee (Hymenoptera: Apidae) Genomes
title_sort	genus-wide characterization of nuclear mitochondrial dnas in bumblebee (hymenoptera: apidae) genomes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4437370c533845c78111d4ca18a1bc7e
work_keys_str_mv	AT leleding genuswidecharacterizationofnuclearmitochondrialdnasinbumblebeehymenopteraapidaegenomes AT huilingsang genuswidecharacterizationofnuclearmitochondrialdnasinbumblebeehymenopteraapidaegenomes AT chengsun genuswidecharacterizationofnuclearmitochondrialdnasinbumblebeehymenopteraapidaegenomes
_version_	1718411739352006656

Genus-Wide Characterization of Nuclear Mitochondrial DNAs in Bumblebee (Hymenoptera: Apidae) Genomes

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