Immunomodulatory effects of the methanolic extract from Pouteria campechiana leaves in macrophage functions

The present study aimed to examine the immunomodulatory properties of the methanolic (MeOH) extract from Pouteria. campechiana leaves in peritoneal macrophages of Balb/c mice. Peritoneal macrophages isolated from mice and Vero cells were treated with the MeOH extract from leaves. Cell viability of t...

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Autores principales:	Ivan Chan-Zapata, Jaqueline Canul-Canche, Karla Fernández-Martín, Zhelmy Martín-Quintal, Julio Cesar Torres-Romero, Julio Cesar Lara-Riegos, Mario Alberto Ramírez-Camacho, Víctor Ermilo Arana-Argáez
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	pouteria campechiana macrophage immunomodulation cytotoxic Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/443834ae33414ede9e9a835c79fe8bbf
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spelling	oai:doaj.org-article:443834ae33414ede9e9a835c79fe8bbf2021-11-26T11:19:46ZImmunomodulatory effects of the methanolic extract from Pouteria campechiana leaves in macrophage functions0954-01051465-344310.1080/09540105.2017.1386163https://doaj.org/article/443834ae33414ede9e9a835c79fe8bbf2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1386163https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443The present study aimed to examine the immunomodulatory properties of the methanolic (MeOH) extract from Pouteria. campechiana leaves in peritoneal macrophages of Balb/c mice. Peritoneal macrophages isolated from mice and Vero cells were treated with the MeOH extract from leaves. Cell viability of the macrophages and Vero cells were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. The phagocytic activity, as nitric oxide (NO), hydrogen peroxide (H2O2), interleukin 6 (IL-6) and tumour necrosis factor α (TNF-α) production were evaluated on peritoneal macrophages. Results showed that the MeOH extract from leaves was able to stimulate the phagocytic activity and increase NO, H2O2 and cytokines production. The viability assays do not show cytotoxic effect on cell viability and cause a significative proliferative effect in the macrophages of a concentration-dependent manner. These results conclude that the MeOH extract from P. campechiana leaves possessed a stronger immunostimulatory effect in a concentration-dependent manner without affect the cell viability.Ivan Chan-ZapataJaqueline Canul-CancheKarla Fernández-MartínZhelmy Martín-QuintalJulio Cesar Torres-RomeroJulio Cesar Lara-RiegosMario Alberto Ramírez-CamachoVíctor Ermilo Arana-ArgáezTaylor & Francis Grouparticlepouteria campechianamacrophageimmunomodulationcytotoxicAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 386-399 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	pouteria campechiana macrophage immunomodulation cytotoxic Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	pouteria campechiana macrophage immunomodulation cytotoxic Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Ivan Chan-Zapata Jaqueline Canul-Canche Karla Fernández-Martín Zhelmy Martín-Quintal Julio Cesar Torres-Romero Julio Cesar Lara-Riegos Mario Alberto Ramírez-Camacho Víctor Ermilo Arana-Argáez Immunomodulatory effects of the methanolic extract from Pouteria campechiana leaves in macrophage functions
description	The present study aimed to examine the immunomodulatory properties of the methanolic (MeOH) extract from Pouteria. campechiana leaves in peritoneal macrophages of Balb/c mice. Peritoneal macrophages isolated from mice and Vero cells were treated with the MeOH extract from leaves. Cell viability of the macrophages and Vero cells were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. The phagocytic activity, as nitric oxide (NO), hydrogen peroxide (H2O2), interleukin 6 (IL-6) and tumour necrosis factor α (TNF-α) production were evaluated on peritoneal macrophages. Results showed that the MeOH extract from leaves was able to stimulate the phagocytic activity and increase NO, H2O2 and cytokines production. The viability assays do not show cytotoxic effect on cell viability and cause a significative proliferative effect in the macrophages of a concentration-dependent manner. These results conclude that the MeOH extract from P. campechiana leaves possessed a stronger immunostimulatory effect in a concentration-dependent manner without affect the cell viability.
format	article
author	Ivan Chan-Zapata Jaqueline Canul-Canche Karla Fernández-Martín Zhelmy Martín-Quintal Julio Cesar Torres-Romero Julio Cesar Lara-Riegos Mario Alberto Ramírez-Camacho Víctor Ermilo Arana-Argáez
author_facet	Ivan Chan-Zapata Jaqueline Canul-Canche Karla Fernández-Martín Zhelmy Martín-Quintal Julio Cesar Torres-Romero Julio Cesar Lara-Riegos Mario Alberto Ramírez-Camacho Víctor Ermilo Arana-Argáez
author_sort	Ivan Chan-Zapata
title	Immunomodulatory effects of the methanolic extract from Pouteria campechiana leaves in macrophage functions
title_short	Immunomodulatory effects of the methanolic extract from Pouteria campechiana leaves in macrophage functions
title_full	Immunomodulatory effects of the methanolic extract from Pouteria campechiana leaves in macrophage functions
title_fullStr	Immunomodulatory effects of the methanolic extract from Pouteria campechiana leaves in macrophage functions
title_full_unstemmed	Immunomodulatory effects of the methanolic extract from Pouteria campechiana leaves in macrophage functions
title_sort	immunomodulatory effects of the methanolic extract from pouteria campechiana leaves in macrophage functions
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/443834ae33414ede9e9a835c79fe8bbf
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Immunomodulatory effects of the methanolic extract from Pouteria campechiana leaves in macrophage functions

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