Evaluation of Anti-Photoaging Effects of a Novel Cosmeceutical Containing a Retinoids Mixture Using In Vitro Cell Models

Physiological ageing due to the passing of time and prolonged exposure to harmful sun rays generate wrinkles and reduce skin elasticity. These visible and clinical signs can be prevented or reversed by known strategies, such as the daily use of cosmetic products with antioxidant combinations or reti...

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Autores principales: Nicola Zerbinati, Sabrina Sommatis, Cristina Maccario, Maria Chiara Capillo, Serena Di Francesco, Elsa Liga, Raffaele Rauso, Edoardo D’Este, Daniela Dalla Gasperina, Stefania Guida, Roberto Mocchi
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Publicado: MDPI AG 2021
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spelling oai:doaj.org-article:4441bfe9b8ec483a87d9a8c5f03329652021-11-11T15:04:40ZEvaluation of Anti-Photoaging Effects of a Novel Cosmeceutical Containing a Retinoids Mixture Using In Vitro Cell Models10.3390/app112199922076-3417https://doaj.org/article/4441bfe9b8ec483a87d9a8c5f03329652021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/9992https://doaj.org/toc/2076-3417Physiological ageing due to the passing of time and prolonged exposure to harmful sun rays generate wrinkles and reduce skin elasticity. These visible and clinical signs can be prevented or reversed by known strategies, such as the daily use of cosmetic products with antioxidant combinations or retinoids. A new dermocosmetic formulation enriched with a complex of retinoids, called RETINOIDS SERUM, was investigated through in vitro assays using human skin cells. The experiments were carried out to assess the anti-ageing activity in normal human dermal fibroblasts (NHDF) and epidermal keratinocytes (HaCaT). After the preliminary MTT assay, the proliferation together with the synthesis of collagen and elastin fibers was performed on NHDF cells after 24 h treatment with the two non-cytotoxic concentrations. Using UVB-irradiated HaCaT cells, the measurement of matrix metalloproteinase-1 (MMP-1) levels was also investigated. In vitro studies show that the dermocosmetic product improves collagen and elastin synthesis and the renewal of dermal fibroblasts. Moreover, a reduction in the MMP-1 secretion was also highlighted in UVB-irradiated HaCaT cells. These results suggest that the cosmetic formulation containing functional compounds such as retinoids can be useful to prevent the natural sign of ageing.Nicola ZerbinatiSabrina SommatisCristina MaccarioMaria Chiara CapilloSerena Di FrancescoElsa LigaRaffaele RausoEdoardo D’EsteDaniela Dalla GasperinaStefania GuidaRoberto MocchiMDPI AGarticlecell cultureclaim substantiationformulationskin ageingretinoidsphotoprotectionTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 9992, p 9992 (2021)
institution DOAJ
collection DOAJ
language EN
topic cell culture
claim substantiation
formulation
skin ageing
retinoids
photoprotection
Technology
T
Engineering (General). Civil engineering (General)
TA1-2040
Biology (General)
QH301-705.5
Physics
QC1-999
Chemistry
QD1-999
spellingShingle cell culture
claim substantiation
formulation
skin ageing
retinoids
photoprotection
Technology
T
Engineering (General). Civil engineering (General)
TA1-2040
Biology (General)
QH301-705.5
Physics
QC1-999
Chemistry
QD1-999
Nicola Zerbinati
Sabrina Sommatis
Cristina Maccario
Maria Chiara Capillo
Serena Di Francesco
Elsa Liga
Raffaele Rauso
Edoardo D’Este
Daniela Dalla Gasperina
Stefania Guida
Roberto Mocchi
Evaluation of Anti-Photoaging Effects of a Novel Cosmeceutical Containing a Retinoids Mixture Using In Vitro Cell Models
description Physiological ageing due to the passing of time and prolonged exposure to harmful sun rays generate wrinkles and reduce skin elasticity. These visible and clinical signs can be prevented or reversed by known strategies, such as the daily use of cosmetic products with antioxidant combinations or retinoids. A new dermocosmetic formulation enriched with a complex of retinoids, called RETINOIDS SERUM, was investigated through in vitro assays using human skin cells. The experiments were carried out to assess the anti-ageing activity in normal human dermal fibroblasts (NHDF) and epidermal keratinocytes (HaCaT). After the preliminary MTT assay, the proliferation together with the synthesis of collagen and elastin fibers was performed on NHDF cells after 24 h treatment with the two non-cytotoxic concentrations. Using UVB-irradiated HaCaT cells, the measurement of matrix metalloproteinase-1 (MMP-1) levels was also investigated. In vitro studies show that the dermocosmetic product improves collagen and elastin synthesis and the renewal of dermal fibroblasts. Moreover, a reduction in the MMP-1 secretion was also highlighted in UVB-irradiated HaCaT cells. These results suggest that the cosmetic formulation containing functional compounds such as retinoids can be useful to prevent the natural sign of ageing.
format article
author Nicola Zerbinati
Sabrina Sommatis
Cristina Maccario
Maria Chiara Capillo
Serena Di Francesco
Elsa Liga
Raffaele Rauso
Edoardo D’Este
Daniela Dalla Gasperina
Stefania Guida
Roberto Mocchi
author_facet Nicola Zerbinati
Sabrina Sommatis
Cristina Maccario
Maria Chiara Capillo
Serena Di Francesco
Elsa Liga
Raffaele Rauso
Edoardo D’Este
Daniela Dalla Gasperina
Stefania Guida
Roberto Mocchi
author_sort Nicola Zerbinati
title Evaluation of Anti-Photoaging Effects of a Novel Cosmeceutical Containing a Retinoids Mixture Using In Vitro Cell Models
title_short Evaluation of Anti-Photoaging Effects of a Novel Cosmeceutical Containing a Retinoids Mixture Using In Vitro Cell Models
title_full Evaluation of Anti-Photoaging Effects of a Novel Cosmeceutical Containing a Retinoids Mixture Using In Vitro Cell Models
title_fullStr Evaluation of Anti-Photoaging Effects of a Novel Cosmeceutical Containing a Retinoids Mixture Using In Vitro Cell Models
title_full_unstemmed Evaluation of Anti-Photoaging Effects of a Novel Cosmeceutical Containing a Retinoids Mixture Using In Vitro Cell Models
title_sort evaluation of anti-photoaging effects of a novel cosmeceutical containing a retinoids mixture using in vitro cell models
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/4441bfe9b8ec483a87d9a8c5f0332965
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