CuO Bionanocomposite with Enhanced Stability and Antibacterial Activity against Extended-Spectrum Beta-Lactamase Strains

Worldwide, bacterial resistance to beta-lactam antibiotics is the greatest challenge in public health care. To overcome the issue, metal-based nanoparticles were extensively used as an alternative to traditional antibiotics. However, their unstable nature limits their use. In the present study a ver...

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Autores principales:	Hina Qamar, Adil Saeed, Mohammad Owais, Touseef Hussain, Kashif Hussain, Aziz ur Rahman, Sarfraz Ahmed, Sachin Kumar, Zulfiqar Ahmad Khan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nanocomposite copper nanoparticle high-performance liquid chromatography phospholipids antibiotic resistance extended-spectrum beta-lactamases Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
Acceso en línea:	https://doaj.org/article/4463b2e3eca64c568db88f89c0fc84fc
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spelling	oai:doaj.org-article:4463b2e3eca64c568db88f89c0fc84fc2021-11-11T17:54:29ZCuO Bionanocomposite with Enhanced Stability and Antibacterial Activity against Extended-Spectrum Beta-Lactamase Strains10.3390/ma142163361996-1944https://doaj.org/article/4463b2e3eca64c568db88f89c0fc84fc2021-10-01T00:00:00Zhttps://www.mdpi.com/1996-1944/14/21/6336https://doaj.org/toc/1996-1944Worldwide, bacterial resistance to beta-lactam antibiotics is the greatest challenge in public health care. To overcome the issue, metal-based nanoparticles were extensively used as an alternative to traditional antibiotics. However, their unstable nature limits their use. In the present study a very simple, environmentally friendly, one-pot synthesis method that avoids the use of organic solvents has been proposed to design stable, novel nanocomposites. Formulation was done by mixing biogenic copper oxide (CuO) nanomaterial with glycerol and phospholipids isolated from egg yolk in an appropriate ratio at optimum conditions. Characterization was done using dynamic light scattering DLS, Zeta potential, high performance liquid chromatography (HPLC), and transmission electron microscopy (TEM). Further, its antibacterial activity was evaluated against the extended-spectrum beta-lactamase strains based on zone of inhibition and minimal inhibitory concentration (MIC) indices. Results from this study have demonstrated the formulation of stable nanocomposites with a zeta potential of 34.9 mV. TEM results indicated clear dispersed particles with an average of 59.3 ± 5 nm size. Furthermore, HPLC analysis of the egg yolk extract exhibits the presence of phospholipids in the sample and has significance in terms of stability. The newly formed nanocomposite has momentous antibacterial activity with MIC 62.5 μg/mL. The results suggest that it could be a good candidate for drug delivery in terms of bactericidal therapeutic applications.Hina QamarAdil SaeedMohammad OwaisTouseef HussainKashif HussainAziz ur RahmanSarfraz AhmedSachin KumarZulfiqar Ahmad KhanMDPI AGarticlenanocompositecopper nanoparticlehigh-performance liquid chromatographyphospholipidsantibiotic resistanceextended-spectrum beta-lactamasesTechnologyTElectrical engineering. Electronics. Nuclear engineeringTK1-9971Engineering (General). Civil engineering (General)TA1-2040MicroscopyQH201-278.5Descriptive and experimental mechanicsQC120-168.85ENMaterials, Vol 14, Iss 6336, p 6336 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanocomposite copper nanoparticle high-performance liquid chromatography phospholipids antibiotic resistance extended-spectrum beta-lactamases Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
spellingShingle	nanocomposite copper nanoparticle high-performance liquid chromatography phospholipids antibiotic resistance extended-spectrum beta-lactamases Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 Hina Qamar Adil Saeed Mohammad Owais Touseef Hussain Kashif Hussain Aziz ur Rahman Sarfraz Ahmed Sachin Kumar Zulfiqar Ahmad Khan CuO Bionanocomposite with Enhanced Stability and Antibacterial Activity against Extended-Spectrum Beta-Lactamase Strains
description	Worldwide, bacterial resistance to beta-lactam antibiotics is the greatest challenge in public health care. To overcome the issue, metal-based nanoparticles were extensively used as an alternative to traditional antibiotics. However, their unstable nature limits their use. In the present study a very simple, environmentally friendly, one-pot synthesis method that avoids the use of organic solvents has been proposed to design stable, novel nanocomposites. Formulation was done by mixing biogenic copper oxide (CuO) nanomaterial with glycerol and phospholipids isolated from egg yolk in an appropriate ratio at optimum conditions. Characterization was done using dynamic light scattering DLS, Zeta potential, high performance liquid chromatography (HPLC), and transmission electron microscopy (TEM). Further, its antibacterial activity was evaluated against the extended-spectrum beta-lactamase strains based on zone of inhibition and minimal inhibitory concentration (MIC) indices. Results from this study have demonstrated the formulation of stable nanocomposites with a zeta potential of 34.9 mV. TEM results indicated clear dispersed particles with an average of 59.3 ± 5 nm size. Furthermore, HPLC analysis of the egg yolk extract exhibits the presence of phospholipids in the sample and has significance in terms of stability. The newly formed nanocomposite has momentous antibacterial activity with MIC 62.5 μg/mL. The results suggest that it could be a good candidate for drug delivery in terms of bactericidal therapeutic applications.
format	article
author	Hina Qamar Adil Saeed Mohammad Owais Touseef Hussain Kashif Hussain Aziz ur Rahman Sarfraz Ahmed Sachin Kumar Zulfiqar Ahmad Khan
author_facet	Hina Qamar Adil Saeed Mohammad Owais Touseef Hussain Kashif Hussain Aziz ur Rahman Sarfraz Ahmed Sachin Kumar Zulfiqar Ahmad Khan
author_sort	Hina Qamar
title	CuO Bionanocomposite with Enhanced Stability and Antibacterial Activity against Extended-Spectrum Beta-Lactamase Strains
title_short	CuO Bionanocomposite with Enhanced Stability and Antibacterial Activity against Extended-Spectrum Beta-Lactamase Strains
title_full	CuO Bionanocomposite with Enhanced Stability and Antibacterial Activity against Extended-Spectrum Beta-Lactamase Strains
title_fullStr	CuO Bionanocomposite with Enhanced Stability and Antibacterial Activity against Extended-Spectrum Beta-Lactamase Strains
title_full_unstemmed	CuO Bionanocomposite with Enhanced Stability and Antibacterial Activity against Extended-Spectrum Beta-Lactamase Strains
title_sort	cuo bionanocomposite with enhanced stability and antibacterial activity against extended-spectrum beta-lactamase strains
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4463b2e3eca64c568db88f89c0fc84fc
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CuO Bionanocomposite with Enhanced Stability and Antibacterial Activity against Extended-Spectrum Beta-Lactamase Strains

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