Mycorrhizal Compatibility and Germination-Promoting Activity of Tulasnella Species in Two Species of Orchid (Cymbidium mannii and Epidendrum radicans)

In nature, Orchidaceae seeds establish a relationship with orchid mycorrhizal fungi to obtain essential nutrients for germination. The orchids, Cymbidium mannii and Epidendrum radicans, have significant ornamental and economic value. We isolated and cultured myc...

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Autores principales:	Qianyu Yang, Lijun Xu, Wei Xia, Lixiong Liang, Xiao Bai, Lubin Li, Lu Xu, Lei Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Cymbidium mannii</i> <i>Epidendrum radicans</i> <i>Tulasnella</i> spp. mycorrhizal fungi symbiosis Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/446bfd399d504cf0aa87f2fd68333202
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spelling	oai:doaj.org-article:446bfd399d504cf0aa87f2fd683332022021-11-25T17:47:28ZMycorrhizal Compatibility and Germination-Promoting Activity of <i>Tulasnella</i> Species in Two Species of Orchid (<i>Cymbidium mannii</i> and <i>Epidendrum radicans</i>)10.3390/horticulturae71104722311-7524https://doaj.org/article/446bfd399d504cf0aa87f2fd683332022021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/472https://doaj.org/toc/2311-7524In nature, Orchidaceae seeds establish a relationship with orchid mycorrhizal fungi to obtain essential nutrients for germination. The orchids, <i>Cymbidium mannii</i> and <i>Epidendrum radicans</i>, have significant ornamental and economic value. We isolated and cultured mycorrhizal fungi from <i>C. mannii</i>, <i>E. radicans,</i> and <i>C. goeringii</i> roots. Three strains of fungi, <i>Tulasnella calospora</i> (Tca), <i>T. asymmetrica</i> (Tas), and <i>T. bifrons</i> (Tbi), were identified using ITS-rDNA sequencing. Their mycorrhizal compatibility, germination-promoting effects, and symbiosis with the seeds of <i>C. mannii</i> and <i>E. radicans</i> were studied in vitro using various concentrations of oatmeal agar (OA) medium. Tca exhibited significant seed-germination-promoting effects on <i>C. mannii</i> (92.1%) and <i>E. radicans</i> (84.7%) on 2.0 and 4.0 g/L OA, respectively. For Tbi and Tas, the highest germination percentages were observed on 4.0 g/L OA in <i>E. radicans</i> (73.60% and 76.49%, respectively). Seed germination in <i>C. mannii</i> was enhanced by high oatmeal concentrations (8.0 and 12.0 g/L) during symbiosis with Tas, whereas Tbi had no effect regardless of OA concentration. Tca exhibited high compatibility with <i>C. mannii</i> and <i>E. radicans</i>, and the oatmeal concentration of the medium affected this compatibility. The findings of this study will aid in the propagation of endangered orchid species for conservation and commercial purposes using mycorrhizal technology.Qianyu YangLijun XuWei XiaLixiong LiangXiao BaiLubin LiLu XuLei LiuMDPI AGarticle<i>Cymbidium mannii</i><i>Epidendrum radicans</i><i>Tulasnella</i> spp.mycorrhizal fungisymbiosisPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 472, p 472 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Cymbidium mannii</i> <i>Epidendrum radicans</i> <i>Tulasnella</i> spp. mycorrhizal fungi symbiosis Plant culture SB1-1110
spellingShingle	<i>Cymbidium mannii</i> <i>Epidendrum radicans</i> <i>Tulasnella</i> spp. mycorrhizal fungi symbiosis Plant culture SB1-1110 Qianyu Yang Lijun Xu Wei Xia Lixiong Liang Xiao Bai Lubin Li Lu Xu Lei Liu Mycorrhizal Compatibility and Germination-Promoting Activity of <i>Tulasnella</i> Species in Two Species of Orchid (<i>Cymbidium mannii</i> and <i>Epidendrum radicans</i>)
description	In nature, Orchidaceae seeds establish a relationship with orchid mycorrhizal fungi to obtain essential nutrients for germination. The orchids, <i>Cymbidium mannii</i> and <i>Epidendrum radicans</i>, have significant ornamental and economic value. We isolated and cultured mycorrhizal fungi from <i>C. mannii</i>, <i>E. radicans,</i> and <i>C. goeringii</i> roots. Three strains of fungi, <i>Tulasnella calospora</i> (Tca), <i>T. asymmetrica</i> (Tas), and <i>T. bifrons</i> (Tbi), were identified using ITS-rDNA sequencing. Their mycorrhizal compatibility, germination-promoting effects, and symbiosis with the seeds of <i>C. mannii</i> and <i>E. radicans</i> were studied in vitro using various concentrations of oatmeal agar (OA) medium. Tca exhibited significant seed-germination-promoting effects on <i>C. mannii</i> (92.1%) and <i>E. radicans</i> (84.7%) on 2.0 and 4.0 g/L OA, respectively. For Tbi and Tas, the highest germination percentages were observed on 4.0 g/L OA in <i>E. radicans</i> (73.60% and 76.49%, respectively). Seed germination in <i>C. mannii</i> was enhanced by high oatmeal concentrations (8.0 and 12.0 g/L) during symbiosis with Tas, whereas Tbi had no effect regardless of OA concentration. Tca exhibited high compatibility with <i>C. mannii</i> and <i>E. radicans</i>, and the oatmeal concentration of the medium affected this compatibility. The findings of this study will aid in the propagation of endangered orchid species for conservation and commercial purposes using mycorrhizal technology.
format	article
author	Qianyu Yang Lijun Xu Wei Xia Lixiong Liang Xiao Bai Lubin Li Lu Xu Lei Liu
author_facet	Qianyu Yang Lijun Xu Wei Xia Lixiong Liang Xiao Bai Lubin Li Lu Xu Lei Liu
author_sort	Qianyu Yang
title	Mycorrhizal Compatibility and Germination-Promoting Activity of <i>Tulasnella</i> Species in Two Species of Orchid (<i>Cymbidium mannii</i> and <i>Epidendrum radicans</i>)
title_short	Mycorrhizal Compatibility and Germination-Promoting Activity of <i>Tulasnella</i> Species in Two Species of Orchid (<i>Cymbidium mannii</i> and <i>Epidendrum radicans</i>)
title_full	Mycorrhizal Compatibility and Germination-Promoting Activity of <i>Tulasnella</i> Species in Two Species of Orchid (<i>Cymbidium mannii</i> and <i>Epidendrum radicans</i>)
title_fullStr	Mycorrhizal Compatibility and Germination-Promoting Activity of <i>Tulasnella</i> Species in Two Species of Orchid (<i>Cymbidium mannii</i> and <i>Epidendrum radicans</i>)
title_full_unstemmed	Mycorrhizal Compatibility and Germination-Promoting Activity of <i>Tulasnella</i> Species in Two Species of Orchid (<i>Cymbidium mannii</i> and <i>Epidendrum radicans</i>)
title_sort	mycorrhizal compatibility and germination-promoting activity of <i>tulasnella</i> species in two species of orchid (<i>cymbidium mannii</i> and <i>epidendrum radicans</i>)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/446bfd399d504cf0aa87f2fd68333202
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Mycorrhizal Compatibility and Germination-Promoting Activity of <i>Tulasnella</i> Species in Two Species of Orchid (<i>Cymbidium mannii</i> and <i>Epidendrum radicans</i>)

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