Bacterial-mediated RNAi and functional analysis of Natalisin in a moth

Abstract The neuropeptide natalisin (NTL) has been determined to play essential roles in reproduction in two Diptera and one Coleoptera species. Whether NTL has similar or even different functions in Lepidoptera remains to be determined. Here, we cloned the NTL transcript in the common cutworm moth...

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Autores principales:	Xia-Fei Wang, Zhe Chen, Xu-Bo Wang, Jin Xu, Peng Chen, Hui Ye
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4475312576fa40669464563b543951a9
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spelling	oai:doaj.org-article:4475312576fa40669464563b543951a92021-12-02T15:53:45ZBacterial-mediated RNAi and functional analysis of Natalisin in a moth10.1038/s41598-021-84104-02045-2322https://doaj.org/article/4475312576fa40669464563b543951a92021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84104-0https://doaj.org/toc/2045-2322Abstract The neuropeptide natalisin (NTL) has been determined to play essential roles in reproduction in two Diptera and one Coleoptera species. Whether NTL has similar or even different functions in Lepidoptera remains to be determined. Here, we cloned the NTL transcript in the common cutworm moth Spodoptera litura. This transcript encodes a 438-amino acid protein. Twelve putative Sl-NTL neuropeptides were defined by cleavage sites. These NTL peptides share a DDPFWxxRamide C-terminal motif. The expressions of Sl-NTL is low during the egg and larval stages, which increased to a higher level during the pupal stage, and then reached the maximum during the adult stage. Moreover, the expression pattern during the pupal stage is similar between sexes while during the adult stage, it is dimorphic. To explore the function of Sl-NTL and assess its potential as a target for pest control, we knocked down the expression of Sl-NTL in both sexes by using bacteria-mediated RNAi. This technique significantly down regulated (reduced up to 83%) the expression of Sl-NTL in both sexes. Knocking down Sl-NTL expression did not significantly affect its development, survival and morphology but significantly reduced adults’ reproductive behavior (including female calling, male courtship, mating and remating patterns and rates) and reproductive output (offspring gain reduced more than 70%).Xia-Fei WangZhe ChenXu-Bo WangJin XuPeng ChenHui YeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xia-Fei Wang Zhe Chen Xu-Bo Wang Jin Xu Peng Chen Hui Ye Bacterial-mediated RNAi and functional analysis of Natalisin in a moth
description	Abstract The neuropeptide natalisin (NTL) has been determined to play essential roles in reproduction in two Diptera and one Coleoptera species. Whether NTL has similar or even different functions in Lepidoptera remains to be determined. Here, we cloned the NTL transcript in the common cutworm moth Spodoptera litura. This transcript encodes a 438-amino acid protein. Twelve putative Sl-NTL neuropeptides were defined by cleavage sites. These NTL peptides share a DDPFWxxRamide C-terminal motif. The expressions of Sl-NTL is low during the egg and larval stages, which increased to a higher level during the pupal stage, and then reached the maximum during the adult stage. Moreover, the expression pattern during the pupal stage is similar between sexes while during the adult stage, it is dimorphic. To explore the function of Sl-NTL and assess its potential as a target for pest control, we knocked down the expression of Sl-NTL in both sexes by using bacteria-mediated RNAi. This technique significantly down regulated (reduced up to 83%) the expression of Sl-NTL in both sexes. Knocking down Sl-NTL expression did not significantly affect its development, survival and morphology but significantly reduced adults’ reproductive behavior (including female calling, male courtship, mating and remating patterns and rates) and reproductive output (offspring gain reduced more than 70%).
format	article
author	Xia-Fei Wang Zhe Chen Xu-Bo Wang Jin Xu Peng Chen Hui Ye
author_facet	Xia-Fei Wang Zhe Chen Xu-Bo Wang Jin Xu Peng Chen Hui Ye
author_sort	Xia-Fei Wang
title	Bacterial-mediated RNAi and functional analysis of Natalisin in a moth
title_short	Bacterial-mediated RNAi and functional analysis of Natalisin in a moth
title_full	Bacterial-mediated RNAi and functional analysis of Natalisin in a moth
title_fullStr	Bacterial-mediated RNAi and functional analysis of Natalisin in a moth
title_full_unstemmed	Bacterial-mediated RNAi and functional analysis of Natalisin in a moth
title_sort	bacterial-mediated rnai and functional analysis of natalisin in a moth
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4475312576fa40669464563b543951a9
work_keys_str_mv	AT xiafeiwang bacterialmediatedrnaiandfunctionalanalysisofnatalisininamoth AT zhechen bacterialmediatedrnaiandfunctionalanalysisofnatalisininamoth AT xubowang bacterialmediatedrnaiandfunctionalanalysisofnatalisininamoth AT jinxu bacterialmediatedrnaiandfunctionalanalysisofnatalisininamoth AT pengchen bacterialmediatedrnaiandfunctionalanalysisofnatalisininamoth AT huiye bacterialmediatedrnaiandfunctionalanalysisofnatalisininamoth
_version_	1718385506262188032

Bacterial-mediated RNAi and functional analysis of Natalisin in a moth

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