Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice

Chien-Ling Su,1,2 Tzu-Tao Chen,1,3 Chih-Cheng Chang,1,3 Kai-Jen Chuang,4,5 Cheng-Kuan Wu,6 Wen-Te Liu,1,2 Kin Fai Ho,7 Kang-Yun Lee,1,8 Shu-Chuan Ho,2,8 Hsiu-Er Tseng,9 Hsiao-Chi Chuang,1,2 Tsun-Jen Cheng6,10 On behalf of the Taiwan CardioPulmonary Research Group (T-CPR) 1Division of Pulmonary Medic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Su CL, Chen TT, Chang CC, Chuang KJ, Wu CK, Liu WT, Ho KF, Lee KY, Ho SC, Tseng HE, Chuang HC, Cheng TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/44774dc15def4a2e9f3b8c320f482822
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:44774dc15def4a2e9f3b8c320f482822
record_format	dspace
spelling	oai:doaj.org-article:44774dc15def4a2e9f3b8c320f4828222021-12-02T02:28:06ZComparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice1176-91141178-2013https://doaj.org/article/44774dc15def4a2e9f3b8c320f4828222013-08-01T00:00:00Zhttp://www.dovepress.com/comparative-proteomics-of-inhaled-silver-nanoparticles-in-healthy-and--a13884https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Chien-Ling Su,1,2 Tzu-Tao Chen,1,3 Chih-Cheng Chang,1,3 Kai-Jen Chuang,4,5 Cheng-Kuan Wu,6 Wen-Te Liu,1,2 Kin Fai Ho,7 Kang-Yun Lee,1,8 Shu-Chuan Ho,2,8 Hsiu-Er Tseng,9 Hsiao-Chi Chuang,1,2 Tsun-Jen Cheng6,10 On behalf of the Taiwan CardioPulmonary Research Group (T-CPR) 1Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, 2School of Respiratory Therapy, College of Medicine, 3Graduate Institute of Clinical Medicine, College of Medicine, 4Department of Public Health, School of Medicine, College of Medicine, 5School of Public Health, College of Public Health and Nutrition, Taipei Medical University, 6Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan; 7School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong, People's Republic of China; 8Department of Thoracic Medicine, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, 9Division of Consultation and Promotion, Taiwan Drug Relief Foundation, 10Department of Public Health, College of Public Health, National Taiwan University, Taipei, Taiwan Background: Silver nanoparticles (AgNPs) have been associated with the exacerbation of asthma; however, the immunological basis for the adjuvant effects of AgNPs is not well understood. Objective: The aim of the study reported here was to investigate the allergic effects of AgNP inhalation using proteomic approaches. Methods: Allergen provoked mice were exposed to 33 nm AgNPs at 3.3 mg/m3. Following this, bronchoalveolar lavage fluid (BALF) and plasma were collected to determine protein profiles. Results: In total, 106 and 79 AgNP-unique proteins were identified in the BALF of control and allergic mice, respectively. Additionally, 40 and 26 AgNP-unique proteins were found in the plasma of control and allergic mice, respectively. The BALF and plasma protein profiles suggested that metabolic, cellular, and immune system processes were associated with pulmonary exposure to AgNPs. In addition, we observed 18 proteins associated with systemic lupus erythematosus that were commonly expressed in both control and allergic mice after AgNP exposure. Significant allergy responses were observed after AgNP exposure in control and allergic mice, as determined by ovalbumin-specific immunoglobulin E. Conclusion: Inhaled AgNPs may regulate immune responses in the lungs of both control and allergic mice. Our results suggest that immunology is a vital response to AgNPs. Keywords: bronchoalveolar lavage, immunotoxicology, proteome, systemic lupus erythematosus, serumSu CLChen TTChang CCChuang KJWu CKLiu WTHo KFLee KYHo SCTseng HEChuang HCCheng TJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2783-2799 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Su CL Chen TT Chang CC Chuang KJ Wu CK Liu WT Ho KF Lee KY Ho SC Tseng HE Chuang HC Cheng TJ Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice
description	Chien-Ling Su,1,2 Tzu-Tao Chen,1,3 Chih-Cheng Chang,1,3 Kai-Jen Chuang,4,5 Cheng-Kuan Wu,6 Wen-Te Liu,1,2 Kin Fai Ho,7 Kang-Yun Lee,1,8 Shu-Chuan Ho,2,8 Hsiu-Er Tseng,9 Hsiao-Chi Chuang,1,2 Tsun-Jen Cheng6,10 On behalf of the Taiwan CardioPulmonary Research Group (T-CPR) 1Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, 2School of Respiratory Therapy, College of Medicine, 3Graduate Institute of Clinical Medicine, College of Medicine, 4Department of Public Health, School of Medicine, College of Medicine, 5School of Public Health, College of Public Health and Nutrition, Taipei Medical University, 6Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan; 7School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong, People's Republic of China; 8Department of Thoracic Medicine, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, 9Division of Consultation and Promotion, Taiwan Drug Relief Foundation, 10Department of Public Health, College of Public Health, National Taiwan University, Taipei, Taiwan Background: Silver nanoparticles (AgNPs) have been associated with the exacerbation of asthma; however, the immunological basis for the adjuvant effects of AgNPs is not well understood. Objective: The aim of the study reported here was to investigate the allergic effects of AgNP inhalation using proteomic approaches. Methods: Allergen provoked mice were exposed to 33 nm AgNPs at 3.3 mg/m3. Following this, bronchoalveolar lavage fluid (BALF) and plasma were collected to determine protein profiles. Results: In total, 106 and 79 AgNP-unique proteins were identified in the BALF of control and allergic mice, respectively. Additionally, 40 and 26 AgNP-unique proteins were found in the plasma of control and allergic mice, respectively. The BALF and plasma protein profiles suggested that metabolic, cellular, and immune system processes were associated with pulmonary exposure to AgNPs. In addition, we observed 18 proteins associated with systemic lupus erythematosus that were commonly expressed in both control and allergic mice after AgNP exposure. Significant allergy responses were observed after AgNP exposure in control and allergic mice, as determined by ovalbumin-specific immunoglobulin E. Conclusion: Inhaled AgNPs may regulate immune responses in the lungs of both control and allergic mice. Our results suggest that immunology is a vital response to AgNPs. Keywords: bronchoalveolar lavage, immunotoxicology, proteome, systemic lupus erythematosus, serum
format	article
author	Su CL Chen TT Chang CC Chuang KJ Wu CK Liu WT Ho KF Lee KY Ho SC Tseng HE Chuang HC Cheng TJ
author_facet	Su CL Chen TT Chang CC Chuang KJ Wu CK Liu WT Ho KF Lee KY Ho SC Tseng HE Chuang HC Cheng TJ
author_sort	Su CL
title	Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice
title_short	Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice
title_full	Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice
title_fullStr	Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice
title_full_unstemmed	Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice
title_sort	comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/44774dc15def4a2e9f3b8c320f482822
work_keys_str_mv	AT sucl comparativeproteomicsofinhaledsilvernanoparticlesinhealthyandallergenprovokedmice AT chentt comparativeproteomicsofinhaledsilvernanoparticlesinhealthyandallergenprovokedmice AT changcc comparativeproteomicsofinhaledsilvernanoparticlesinhealthyandallergenprovokedmice AT chuangkj comparativeproteomicsofinhaledsilvernanoparticlesinhealthyandallergenprovokedmice AT wuck comparativeproteomicsofinhaledsilvernanoparticlesinhealthyandallergenprovokedmice AT liuwt comparativeproteomicsofinhaledsilvernanoparticlesinhealthyandallergenprovokedmice AT hokf comparativeproteomicsofinhaledsilvernanoparticlesinhealthyandallergenprovokedmice AT leeky comparativeproteomicsofinhaledsilvernanoparticlesinhealthyandallergenprovokedmice AT hosc comparativeproteomicsofinhaledsilvernanoparticlesinhealthyandallergenprovokedmice AT tsenghe comparativeproteomicsofinhaledsilvernanoparticlesinhealthyandallergenprovokedmice AT chuanghc comparativeproteomicsofinhaledsilvernanoparticlesinhealthyandallergenprovokedmice AT chengtj comparativeproteomicsofinhaledsilvernanoparticlesinhealthyandallergenprovokedmice
_version_	1718402463575310336

Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice

Ejemplares similares