Differential responses of cecal microbiota to fishmeal, Eimeria and Clostridium perfringens in a necrotic enteritis challenge model in chickens.

Clostridium perfringens causes enteric diseases in animals and humans. In poultry, avian-specific C. perfringens strains cause necrotic enteritis, an economically significant poultry disease that costs the global industry over $2 billion annually in losses and control measures. With removal of antib...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dragana Stanley, Shu-Biao Wu, Nicholas Rodgers, Robert A Swick, Robert J Moore
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/447f8ccf723d43178f9422f9b91ebad9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:447f8ccf723d43178f9422f9b91ebad9
record_format	dspace
spelling	oai:doaj.org-article:447f8ccf723d43178f9422f9b91ebad92021-11-25T06:02:46ZDifferential responses of cecal microbiota to fishmeal, Eimeria and Clostridium perfringens in a necrotic enteritis challenge model in chickens.1932-620310.1371/journal.pone.0104739https://doaj.org/article/447f8ccf723d43178f9422f9b91ebad92014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25167074/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Clostridium perfringens causes enteric diseases in animals and humans. In poultry, avian-specific C. perfringens strains cause necrotic enteritis, an economically significant poultry disease that costs the global industry over $2 billion annually in losses and control measures. With removal of antibiotic growth promoters in some countries this disease appears to be on the rise. In experimental conditions used to study disease pathogenesis and potential control measures, reproduction of the disease relies on the use of predisposing factors such as Eimeria infection and the use of high protein diets, indicating complex mechanisms involved in the onset of necrotic enteritis. The mechanisms by which the predisposing factors contribute to disease progression are not well understood but it has been suggested that they may cause perturbations in the microbiota within the gastrointestinal tract. We inspected changes in cecal microbiota and short chain fatty acids (SCFA) induced by Eimeria and fishmeal, in birds challenged or not challenged with C. perfringens. C. perfringens challenge in the absence of predisposing factors did not cause significant changes in either the alpha or beta diversity of the microbiota nor in concentrations of SCFA. Moreover, there was no C. perfringens detected in the cecal microbiota 2 days post-challenge without the presence of predisposing factors. In contrast, both fishmeal and Eimeria caused significant changes in microbiota, seen in both alpha and beta diversity and also enabled C. perfringens to establish itself post challenge. Eimeria had its strongest influence on intestinal microbiota and SCFA when combined with fishmeal. Out of 6 SCFAs measured, including butyric acid, none were significantly influenced by C. perfringens, but their levels were strongly modified following the use of both predisposing factors. There was little overlap in the changes caused following Eimeria and fishmeal treatments, possibly indicating multiple routes for progressing towards clinical symptoms of necrotic enteritis.Dragana StanleyShu-Biao WuNicholas RodgersRobert A SwickRobert J MoorePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104739 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dragana Stanley Shu-Biao Wu Nicholas Rodgers Robert A Swick Robert J Moore Differential responses of cecal microbiota to fishmeal, Eimeria and Clostridium perfringens in a necrotic enteritis challenge model in chickens.
description	Clostridium perfringens causes enteric diseases in animals and humans. In poultry, avian-specific C. perfringens strains cause necrotic enteritis, an economically significant poultry disease that costs the global industry over $2 billion annually in losses and control measures. With removal of antibiotic growth promoters in some countries this disease appears to be on the rise. In experimental conditions used to study disease pathogenesis and potential control measures, reproduction of the disease relies on the use of predisposing factors such as Eimeria infection and the use of high protein diets, indicating complex mechanisms involved in the onset of necrotic enteritis. The mechanisms by which the predisposing factors contribute to disease progression are not well understood but it has been suggested that they may cause perturbations in the microbiota within the gastrointestinal tract. We inspected changes in cecal microbiota and short chain fatty acids (SCFA) induced by Eimeria and fishmeal, in birds challenged or not challenged with C. perfringens. C. perfringens challenge in the absence of predisposing factors did not cause significant changes in either the alpha or beta diversity of the microbiota nor in concentrations of SCFA. Moreover, there was no C. perfringens detected in the cecal microbiota 2 days post-challenge without the presence of predisposing factors. In contrast, both fishmeal and Eimeria caused significant changes in microbiota, seen in both alpha and beta diversity and also enabled C. perfringens to establish itself post challenge. Eimeria had its strongest influence on intestinal microbiota and SCFA when combined with fishmeal. Out of 6 SCFAs measured, including butyric acid, none were significantly influenced by C. perfringens, but their levels were strongly modified following the use of both predisposing factors. There was little overlap in the changes caused following Eimeria and fishmeal treatments, possibly indicating multiple routes for progressing towards clinical symptoms of necrotic enteritis.
format	article
author	Dragana Stanley Shu-Biao Wu Nicholas Rodgers Robert A Swick Robert J Moore
author_facet	Dragana Stanley Shu-Biao Wu Nicholas Rodgers Robert A Swick Robert J Moore
author_sort	Dragana Stanley
title	Differential responses of cecal microbiota to fishmeal, Eimeria and Clostridium perfringens in a necrotic enteritis challenge model in chickens.
title_short	Differential responses of cecal microbiota to fishmeal, Eimeria and Clostridium perfringens in a necrotic enteritis challenge model in chickens.
title_full	Differential responses of cecal microbiota to fishmeal, Eimeria and Clostridium perfringens in a necrotic enteritis challenge model in chickens.
title_fullStr	Differential responses of cecal microbiota to fishmeal, Eimeria and Clostridium perfringens in a necrotic enteritis challenge model in chickens.
title_full_unstemmed	Differential responses of cecal microbiota to fishmeal, Eimeria and Clostridium perfringens in a necrotic enteritis challenge model in chickens.
title_sort	differential responses of cecal microbiota to fishmeal, eimeria and clostridium perfringens in a necrotic enteritis challenge model in chickens.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/447f8ccf723d43178f9422f9b91ebad9
work_keys_str_mv	AT draganastanley differentialresponsesofcecalmicrobiotatofishmealeimeriaandclostridiumperfringensinanecroticenteritischallengemodelinchickens AT shubiaowu differentialresponsesofcecalmicrobiotatofishmealeimeriaandclostridiumperfringensinanecroticenteritischallengemodelinchickens AT nicholasrodgers differentialresponsesofcecalmicrobiotatofishmealeimeriaandclostridiumperfringensinanecroticenteritischallengemodelinchickens AT robertaswick differentialresponsesofcecalmicrobiotatofishmealeimeriaandclostridiumperfringensinanecroticenteritischallengemodelinchickens AT robertjmoore differentialresponsesofcecalmicrobiotatofishmealeimeriaandclostridiumperfringensinanecroticenteritischallengemodelinchickens
_version_	1718414218205593600

Differential responses of cecal microbiota to fishmeal, Eimeria and Clostridium perfringens in a necrotic enteritis challenge model in chickens.

Ejemplares similares