Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells

Abstract The immune regulation function of ovotransferrin (OVT) explored using the RAW264.7 was induced by lipopolysaccharide (LPS) as vitro model in this study. The results showed that RAW264.7 cultured with OVT (200 μg/ml) alone not only enhanced the phagocytic activity and the production and expr...

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Autores principales:	Zhiying Ru, Mingsheng Xu, Gaoxiang Zhu, Yonggang Tu, Yan Jiang, Huaying Du
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	inflammation MyD88 ovotransferrin RAW 264.7 macrophage toll‐like receptor 4 Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/44934f808d614a59ae28de6b726b85af
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spelling	oai:doaj.org-article:44934f808d614a59ae28de6b726b85af2021-11-04T13:06:43ZOvotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells2048-717710.1002/fsn3.2569https://doaj.org/article/44934f808d614a59ae28de6b726b85af2021-11-01T00:00:00Zhttps://doi.org/10.1002/fsn3.2569https://doaj.org/toc/2048-7177Abstract The immune regulation function of ovotransferrin (OVT) explored using the RAW264.7 was induced by lipopolysaccharide (LPS) as vitro model in this study. The results showed that RAW264.7 cultured with OVT (200 μg/ml) alone not only enhanced the phagocytic activity and the production and expression of inflammatory factors, but also expression of toll‐like receptor 4 (TLR4) gene was significantly promoted by OVT. OVT (50 μg/ml) significantly inhibited the secretion and expression of inflammatory factors in LPS‐stimulated RAW264.7, but CD14 and TLR4 genes expressions were no obvious effects. Inflammatory cytokines and NO secreted by OVT‐induced macrophages pretreated with inhibitors of TLR4 were down‐regulated. We further verified the effects of OVT on inflammatory signaling pathway‐related proteins through immunofluorescence and western blotting, MyD88, TLR4 and the phosphorylation of IκBα and p65 were significantly promoted by OVT, but there was no significant effects on the phosphorylation of IRF3. OVT promoted the phosphorylation of ERK and p38 in RAW264.7 and inhibited the phosphorylated expression of MAPK in LPS‐mediated inflammation. These results indicated that OVT had the bidirectional immunoregulatory function through TLR4‐mediated NF‐κB/MAPK signaling pathway, that is, anti‐inflammatory effect of low concentration and immune‐enhancing activity of high concentration were showed. That provides a theoretical utilization for the development and utilization of OVT.Zhiying RuMingsheng XuGaoxiang ZhuYonggang TuYan JiangHuaying DuWileyarticleinflammationMyD88ovotransferrinRAW 264.7 macrophagetoll‐like receptor 4Nutrition. Foods and food supplyTX341-641ENFood Science & Nutrition, Vol 9, Iss 11, Pp 6162-6175 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	inflammation MyD88 ovotransferrin RAW 264.7 macrophage toll‐like receptor 4 Nutrition. Foods and food supply TX341-641
spellingShingle	inflammation MyD88 ovotransferrin RAW 264.7 macrophage toll‐like receptor 4 Nutrition. Foods and food supply TX341-641 Zhiying Ru Mingsheng Xu Gaoxiang Zhu Yonggang Tu Yan Jiang Huaying Du Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells
description	Abstract The immune regulation function of ovotransferrin (OVT) explored using the RAW264.7 was induced by lipopolysaccharide (LPS) as vitro model in this study. The results showed that RAW264.7 cultured with OVT (200 μg/ml) alone not only enhanced the phagocytic activity and the production and expression of inflammatory factors, but also expression of toll‐like receptor 4 (TLR4) gene was significantly promoted by OVT. OVT (50 μg/ml) significantly inhibited the secretion and expression of inflammatory factors in LPS‐stimulated RAW264.7, but CD14 and TLR4 genes expressions were no obvious effects. Inflammatory cytokines and NO secreted by OVT‐induced macrophages pretreated with inhibitors of TLR4 were down‐regulated. We further verified the effects of OVT on inflammatory signaling pathway‐related proteins through immunofluorescence and western blotting, MyD88, TLR4 and the phosphorylation of IκBα and p65 were significantly promoted by OVT, but there was no significant effects on the phosphorylation of IRF3. OVT promoted the phosphorylation of ERK and p38 in RAW264.7 and inhibited the phosphorylated expression of MAPK in LPS‐mediated inflammation. These results indicated that OVT had the bidirectional immunoregulatory function through TLR4‐mediated NF‐κB/MAPK signaling pathway, that is, anti‐inflammatory effect of low concentration and immune‐enhancing activity of high concentration were showed. That provides a theoretical utilization for the development and utilization of OVT.
format	article
author	Zhiying Ru Mingsheng Xu Gaoxiang Zhu Yonggang Tu Yan Jiang Huaying Du
author_facet	Zhiying Ru Mingsheng Xu Gaoxiang Zhu Yonggang Tu Yan Jiang Huaying Du
author_sort	Zhiying Ru
title	Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells
title_short	Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells
title_full	Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells
title_fullStr	Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells
title_full_unstemmed	Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells
title_sort	ovotransferrin exerts bidirectional immunomodulatory activities via tlr4‐mediated signal transduction pathways in raw264.7 cells
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/44934f808d614a59ae28de6b726b85af
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_version_	1718444896184958976

Ovotransferrin exerts bidirectional immunomodulatory activities via TLR4‐mediated signal transduction pathways in RAW264.7 cells

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