Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures

Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures

Laura Susana Acosta-Torres,1 Irasema Mendieta,2 Rosa Elvira Nuñez-Anita,3 Marcos Cajero-Juárez,3 Víctor M Castaño41National School of Higher Education, School of Dentistry - Leon Unit, National Autonomus University of Mexico (UNAM), Leon, G...

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Autores principales: Acosta-Torres LS, Mendieta I, Nuñez-Anita RE, Cajero-Juárez M, Castaño VM
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2012
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/449817835017475a8b7152dfafbf26fc
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spelling oai:doaj.org-article:449817835017475a8b7152dfafbf26fc2021-12-02T02:57:24ZCytocompatible antifungal acrylic resin containing silver nanoparticles for dentures1176-91141178-2013https://doaj.org/article/449817835017475a8b7152dfafbf26fc2012-09-01T00:00:00Zhttp://www.dovepress.com/cytocompatible-antifungal-acrylic-resin-containing-silver-nanoparticle-a10883https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Laura Susana Acosta-Torres,1 Irasema Mendieta,2 Rosa Elvira Nuñez-Anita,3 Marcos Cajero-Juárez,3 Víctor M Castaño41National School of Higher Education, School of Dentistry - Leon Unit, National Autonomus University of Mexico (UNAM), Leon, Guanajuato, 2Neurobiology Institute, National Autonomus University of Mexico (UNAM), Juriquilla, Queretaro, 3Animal Biotechnology Laboratory, Faculty of Veterinary Medicine at San Nicolas de Hidalgo, Michoacán University, Michoacán, 4Molecular Materials Department, Applied Physics and Advanced Technology Center, National Autonomus University of Mexico (UNAM), Juriquilla, Queretaro, MexicoBackground: Inhibition of Candida albicans on denture resins could play a significant role in preventing the development of denture stomatitis. The safety of a new dental material with antifungal properties was analyzed in this work.Methods: Poly(methyl methacrylate) [PMMA] discs and PMMA-silver nanoparticle discs were formulated, with the commercial acrylic resin, Nature-CrylTM, used as a control. Silver nanoparticles were synthesized and characterized by ultraviolet-visible spectroscopy, dispersive Raman spectroscopy, and transmission electron microscopy. The antifungal effect was assessed using a luminescent microbial cell viability assay. Biocompatibility tests were carried out using NIH-3T3 mouse embryonic fibroblasts and a Jurkat human lymphocyte cell line. Cells were cultured for 24 or 72 hours in the presence or absence of the polymer formulations and analyzed using three different tests, ie, cellular viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation by enzyme-linked immunosorbent assay BrdU, and genomic DNA damage (Comet assay). Finally, the samples were evaluated mechanically, and the polymer-bearing silver nanoparticles were analyzed microscopically to evaluate dispersion of the nanoparticles.Results: The results show that PMMA-silver nanoparticle discs significantly reduce adherence of C. albicans and do not affect metabolism or proliferation. They also appear not to cause genotoxic damage to cells.Conclusion: The present work has developed a new biocompatible antifungal PMMA denture base material.Keywords: cytotoxicity, genotoxicity, dental polymers, denture bases, Candida albicans, antifungal effectAcosta-Torres LSMendieta INuñez-Anita RECajero-Juárez MCastaño VMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4777-4786 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Acosta-Torres LS
Mendieta I
Nuñez-Anita RE
Cajero-Juárez M
Castaño VM
Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures
description Laura Susana Acosta-Torres,1 Irasema Mendieta,2 Rosa Elvira Nuñez-Anita,3 Marcos Cajero-Juárez,3 Víctor M Castaño41National School of Higher Education, School of Dentistry - Leon Unit, National Autonomus University of Mexico (UNAM), Leon, Guanajuato, 2Neurobiology Institute, National Autonomus University of Mexico (UNAM), Juriquilla, Queretaro, 3Animal Biotechnology Laboratory, Faculty of Veterinary Medicine at San Nicolas de Hidalgo, Michoacán University, Michoacán, 4Molecular Materials Department, Applied Physics and Advanced Technology Center, National Autonomus University of Mexico (UNAM), Juriquilla, Queretaro, MexicoBackground: Inhibition of Candida albicans on denture resins could play a significant role in preventing the development of denture stomatitis. The safety of a new dental material with antifungal properties was analyzed in this work.Methods: Poly(methyl methacrylate) [PMMA] discs and PMMA-silver nanoparticle discs were formulated, with the commercial acrylic resin, Nature-CrylTM, used as a control. Silver nanoparticles were synthesized and characterized by ultraviolet-visible spectroscopy, dispersive Raman spectroscopy, and transmission electron microscopy. The antifungal effect was assessed using a luminescent microbial cell viability assay. Biocompatibility tests were carried out using NIH-3T3 mouse embryonic fibroblasts and a Jurkat human lymphocyte cell line. Cells were cultured for 24 or 72 hours in the presence or absence of the polymer formulations and analyzed using three different tests, ie, cellular viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation by enzyme-linked immunosorbent assay BrdU, and genomic DNA damage (Comet assay). Finally, the samples were evaluated mechanically, and the polymer-bearing silver nanoparticles were analyzed microscopically to evaluate dispersion of the nanoparticles.Results: The results show that PMMA-silver nanoparticle discs significantly reduce adherence of C. albicans and do not affect metabolism or proliferation. They also appear not to cause genotoxic damage to cells.Conclusion: The present work has developed a new biocompatible antifungal PMMA denture base material.Keywords: cytotoxicity, genotoxicity, dental polymers, denture bases, Candida albicans, antifungal effect
format article
author Acosta-Torres LS
Mendieta I
Nuñez-Anita RE
Cajero-Juárez M
Castaño VM
author_facet Acosta-Torres LS
Mendieta I
Nuñez-Anita RE
Cajero-Juárez M
Castaño VM
author_sort Acosta-Torres LS
title Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures
title_short Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures
title_full Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures
title_fullStr Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures
title_full_unstemmed Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures
title_sort cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/449817835017475a8b7152dfafbf26fc
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