Transcriptome analysis of Dastarcus helophoroides (Coleoptera: Bothrideridae) using Illumina HiSeq sequencing.

<h4>Background</h4>Dastarcus helophoroides is known as the most valuable natural enemy insect against many large-body longhorned beetles. The molecular mechanism of its long lifespan and reproduction makes it a unique resource for genomic research. However, molecular biological studies o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wei Zhang, Wang Song, Zhengqing Zhang, Haidong Wang, Miaomiao Yang, Ruijian Guo, Menglou Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/44987ea88ba1475a86215876b33b886f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:44987ea88ba1475a86215876b33b886f
record_format	dspace
spelling	oai:doaj.org-article:44987ea88ba1475a86215876b33b886f2021-11-25T06:10:18ZTranscriptome analysis of Dastarcus helophoroides (Coleoptera: Bothrideridae) using Illumina HiSeq sequencing.1932-620310.1371/journal.pone.0100673https://doaj.org/article/44987ea88ba1475a86215876b33b886f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24979346/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Dastarcus helophoroides is known as the most valuable natural enemy insect against many large-body longhorned beetles. The molecular mechanism of its long lifespan and reproduction makes it a unique resource for genomic research. However, molecular biological studies on this parasitic beetle are scarce, and genomic information for D. helophoroides is not currently available. Thus, transcriptome information for this species is an important resource that is required for a better understanding of the molecular mechanisms of D. helophoroides. In this study, we obtained transcriptome information of D. helophoroides using high-throughput RNA sequencing.<h4>Results</h4>Using Illumina HiSeq 2000 sequencing, 27,543,746 clean reads corresponding to a total of 2.48 Gb nucleotides were obtained from a single run. These reads were assembled into 42,810 unigenes with a mean length of 683 bp. Using a sequence similarity search against the five public databases (NR, Swiss-Prot, GO, COG, KEGG) with a cut-off E-value of 10(-5) using Blastx, a total of 31,293 unigenes were annotated with gene description, gene ontology terms, or metabolic pathways.<h4>Conclusions</h4>To the best of our knowledge, this is the first study on the transcriptome information of D. helophoroides. The transcriptome data presented in this study provide comprehensive information for future studies in D. helophoroides, particularly for functional genomic studies in this parasitic beetle.Wei ZhangWang SongZhengqing ZhangHaidong WangMiaomiao YangRuijian GuoMenglou LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100673 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei Zhang Wang Song Zhengqing Zhang Haidong Wang Miaomiao Yang Ruijian Guo Menglou Li Transcriptome analysis of Dastarcus helophoroides (Coleoptera: Bothrideridae) using Illumina HiSeq sequencing.
description	<h4>Background</h4>Dastarcus helophoroides is known as the most valuable natural enemy insect against many large-body longhorned beetles. The molecular mechanism of its long lifespan and reproduction makes it a unique resource for genomic research. However, molecular biological studies on this parasitic beetle are scarce, and genomic information for D. helophoroides is not currently available. Thus, transcriptome information for this species is an important resource that is required for a better understanding of the molecular mechanisms of D. helophoroides. In this study, we obtained transcriptome information of D. helophoroides using high-throughput RNA sequencing.<h4>Results</h4>Using Illumina HiSeq 2000 sequencing, 27,543,746 clean reads corresponding to a total of 2.48 Gb nucleotides were obtained from a single run. These reads were assembled into 42,810 unigenes with a mean length of 683 bp. Using a sequence similarity search against the five public databases (NR, Swiss-Prot, GO, COG, KEGG) with a cut-off E-value of 10(-5) using Blastx, a total of 31,293 unigenes were annotated with gene description, gene ontology terms, or metabolic pathways.<h4>Conclusions</h4>To the best of our knowledge, this is the first study on the transcriptome information of D. helophoroides. The transcriptome data presented in this study provide comprehensive information for future studies in D. helophoroides, particularly for functional genomic studies in this parasitic beetle.
format	article
author	Wei Zhang Wang Song Zhengqing Zhang Haidong Wang Miaomiao Yang Ruijian Guo Menglou Li
author_facet	Wei Zhang Wang Song Zhengqing Zhang Haidong Wang Miaomiao Yang Ruijian Guo Menglou Li
author_sort	Wei Zhang
title	Transcriptome analysis of Dastarcus helophoroides (Coleoptera: Bothrideridae) using Illumina HiSeq sequencing.
title_short	Transcriptome analysis of Dastarcus helophoroides (Coleoptera: Bothrideridae) using Illumina HiSeq sequencing.
title_full	Transcriptome analysis of Dastarcus helophoroides (Coleoptera: Bothrideridae) using Illumina HiSeq sequencing.
title_fullStr	Transcriptome analysis of Dastarcus helophoroides (Coleoptera: Bothrideridae) using Illumina HiSeq sequencing.
title_full_unstemmed	Transcriptome analysis of Dastarcus helophoroides (Coleoptera: Bothrideridae) using Illumina HiSeq sequencing.
title_sort	transcriptome analysis of dastarcus helophoroides (coleoptera: bothrideridae) using illumina hiseq sequencing.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/44987ea88ba1475a86215876b33b886f
work_keys_str_mv	AT weizhang transcriptomeanalysisofdastarcushelophoroidescoleopterabothrideridaeusingilluminahiseqsequencing AT wangsong transcriptomeanalysisofdastarcushelophoroidescoleopterabothrideridaeusingilluminahiseqsequencing AT zhengqingzhang transcriptomeanalysisofdastarcushelophoroidescoleopterabothrideridaeusingilluminahiseqsequencing AT haidongwang transcriptomeanalysisofdastarcushelophoroidescoleopterabothrideridaeusingilluminahiseqsequencing AT miaomiaoyang transcriptomeanalysisofdastarcushelophoroidescoleopterabothrideridaeusingilluminahiseqsequencing AT ruijianguo transcriptomeanalysisofdastarcushelophoroidescoleopterabothrideridaeusingilluminahiseqsequencing AT menglouli transcriptomeanalysisofdastarcushelophoroidescoleopterabothrideridaeusingilluminahiseqsequencing
_version_	1718414096921001984

Transcriptome analysis of Dastarcus helophoroides (Coleoptera: Bothrideridae) using Illumina HiSeq sequencing.

Ejemplares similares