Identification of scleractinian coral recruits using fluorescent censusing and DNA barcoding techniques.

The identification of coral recruits has been problematic due to a lack of definitive morphological characters being available for higher taxonomic resolution. In this study, we tested whether fluorescent detection of coral recruits used in combinations of different DNA-barcoding markers (cytochrome...

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Autores principales:	Chia-Min Hsu, Stéphane de Palmas, Chao-Yang Kuo, Vianney Denis, Chaolun Allen Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/44a062e8e238406b8674b94902c48ce5
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spelling	oai:doaj.org-article:44a062e8e238406b8674b94902c48ce52021-11-25T06:00:56ZIdentification of scleractinian coral recruits using fluorescent censusing and DNA barcoding techniques.1932-620310.1371/journal.pone.0107366https://doaj.org/article/44a062e8e238406b8674b94902c48ce52014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107366https://doaj.org/toc/1932-6203The identification of coral recruits has been problematic due to a lack of definitive morphological characters being available for higher taxonomic resolution. In this study, we tested whether fluorescent detection of coral recruits used in combinations of different DNA-barcoding markers (cytochrome oxidase I gene [COI], open reading frame [ORF], and nuclear Pax-C intron [PaxC]) could be useful for increasing the resolution of coral spat identification in ecological studies. One hundred and fifty settlement plates were emplaced at nine sites on the fringing reefs of Kenting National Park in southern Taiwan between April 2011 and September 2012. A total of 248 living coral spats and juveniles (with basal areas ranging from 0.21 to 134.57 mm(2)) were detected on the plates with the aid of fluorescent light and collected for molecular analyses. Using the COI DNA barcoding technique, 90.3% (224/248) of coral spats were successfully identified into six genera, including Acropora, Isopora, Montipora, Pocillopora, Porites, and Pavona. PaxC further separated I. cuneata and I. palifera of Isopora from Acropora, and ORF successfully identified the species of Pocillopora (except P. meandrina and P. eydouxi). Moreover, other cnidarian species such as actinarians, zoanthids, and Millepora species were visually found using fluorescence and identified by COI DNA barcoding. This combination of existing approaches greatly improved the taxonomic resolution of early coral life stages, which to date has been mainly limited to the family level based on skeletal identification. Overall, this study suggests important improvements for the identification of coral recruits in ecological studies.Chia-Min HsuStéphane de PalmasChao-Yang KuoVianney DenisChaolun Allen ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107366 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chia-Min Hsu Stéphane de Palmas Chao-Yang Kuo Vianney Denis Chaolun Allen Chen Identification of scleractinian coral recruits using fluorescent censusing and DNA barcoding techniques.
description	The identification of coral recruits has been problematic due to a lack of definitive morphological characters being available for higher taxonomic resolution. In this study, we tested whether fluorescent detection of coral recruits used in combinations of different DNA-barcoding markers (cytochrome oxidase I gene [COI], open reading frame [ORF], and nuclear Pax-C intron [PaxC]) could be useful for increasing the resolution of coral spat identification in ecological studies. One hundred and fifty settlement plates were emplaced at nine sites on the fringing reefs of Kenting National Park in southern Taiwan between April 2011 and September 2012. A total of 248 living coral spats and juveniles (with basal areas ranging from 0.21 to 134.57 mm(2)) were detected on the plates with the aid of fluorescent light and collected for molecular analyses. Using the COI DNA barcoding technique, 90.3% (224/248) of coral spats were successfully identified into six genera, including Acropora, Isopora, Montipora, Pocillopora, Porites, and Pavona. PaxC further separated I. cuneata and I. palifera of Isopora from Acropora, and ORF successfully identified the species of Pocillopora (except P. meandrina and P. eydouxi). Moreover, other cnidarian species such as actinarians, zoanthids, and Millepora species were visually found using fluorescence and identified by COI DNA barcoding. This combination of existing approaches greatly improved the taxonomic resolution of early coral life stages, which to date has been mainly limited to the family level based on skeletal identification. Overall, this study suggests important improvements for the identification of coral recruits in ecological studies.
format	article
author	Chia-Min Hsu Stéphane de Palmas Chao-Yang Kuo Vianney Denis Chaolun Allen Chen
author_facet	Chia-Min Hsu Stéphane de Palmas Chao-Yang Kuo Vianney Denis Chaolun Allen Chen
author_sort	Chia-Min Hsu
title	Identification of scleractinian coral recruits using fluorescent censusing and DNA barcoding techniques.
title_short	Identification of scleractinian coral recruits using fluorescent censusing and DNA barcoding techniques.
title_full	Identification of scleractinian coral recruits using fluorescent censusing and DNA barcoding techniques.
title_fullStr	Identification of scleractinian coral recruits using fluorescent censusing and DNA barcoding techniques.
title_full_unstemmed	Identification of scleractinian coral recruits using fluorescent censusing and DNA barcoding techniques.
title_sort	identification of scleractinian coral recruits using fluorescent censusing and dna barcoding techniques.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/44a062e8e238406b8674b94902c48ce5
work_keys_str_mv	AT chiaminhsu identificationofscleractiniancoralrecruitsusingfluorescentcensusinganddnabarcodingtechniques AT stephanedepalmas identificationofscleractiniancoralrecruitsusingfluorescentcensusinganddnabarcodingtechniques AT chaoyangkuo identificationofscleractiniancoralrecruitsusingfluorescentcensusinganddnabarcodingtechniques AT vianneydenis identificationofscleractiniancoralrecruitsusingfluorescentcensusinganddnabarcodingtechniques AT chaolunallenchen identificationofscleractiniancoralrecruitsusingfluorescentcensusinganddnabarcodingtechniques
_version_	1718414277210013696

Identification of scleractinian coral recruits using fluorescent censusing and DNA barcoding techniques.

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