Molecular barcoding of marine ornamental fish from the southern coast of West Java validates conventional identification

Conventional identification of marine ornamental fish has faced difficulties due to similar color patterns of closed related species, or juvenile individuals have different color patterns from adult individuals. Molecular barcoding using the cytochrome c oxidase I (COI) gene provides a reliable tool...

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Autores principales:	Nuryanto Agus, Kusbiyanto Kusbiyanto, Bhagawati Dian
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/44a2b62dfd484c9092531df6f39b7f22
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spelling	oai:doaj.org-article:44a2b62dfd484c9092531df6f39b7f222021-11-12T11:44:42ZMolecular barcoding of marine ornamental fish from the southern coast of West Java validates conventional identification2267-124210.1051/e3sconf/202132201004https://doaj.org/article/44a2b62dfd484c9092531df6f39b7f222021-01-01T00:00:00Zhttps://www.e3s-conferences.org/articles/e3sconf/pdf/2021/98/e3sconf_isffs2021_01004.pdfhttps://doaj.org/toc/2267-1242Conventional identification of marine ornamental fish has faced difficulties due to similar color patterns of closed related species, or juvenile individuals have different color patterns from adult individuals. Molecular barcoding using the cytochrome c oxidase I (COI) gene provides a reliable tool for unmasking such difficulties. This study aimed to barcode marine ornamental fish from the southern coast of West Java. Fragment of the COI gene was sequenced from 54 morphotypes. In this study, we determined the taxonomic status of the samples based on a 5% genetic divergence, with the parameter including sequence percent identity, genetic distance, and length of monophyletic branch in a phylogenetic tree. The result showed that most samples had a high percentage of sequence identities, low genetic distances, and short chapters in monophyletic clades, but the remaining were not. Those data indicated that most samples could be identified at species-level without doubt and support conventional identification. Barcoding success is also depending on the availability of conspecific sequences in the databases. This study concluded that molecular barcoding could strengthen and validate traditional identification.Nuryanto AgusKusbiyanto KusbiyantoBhagawati DianEDP SciencesarticleEnvironmental sciencesGE1-350ENFRE3S Web of Conferences, Vol 322, p 01004 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Environmental sciences GE1-350
spellingShingle	Environmental sciences GE1-350 Nuryanto Agus Kusbiyanto Kusbiyanto Bhagawati Dian Molecular barcoding of marine ornamental fish from the southern coast of West Java validates conventional identification
description	Conventional identification of marine ornamental fish has faced difficulties due to similar color patterns of closed related species, or juvenile individuals have different color patterns from adult individuals. Molecular barcoding using the cytochrome c oxidase I (COI) gene provides a reliable tool for unmasking such difficulties. This study aimed to barcode marine ornamental fish from the southern coast of West Java. Fragment of the COI gene was sequenced from 54 morphotypes. In this study, we determined the taxonomic status of the samples based on a 5% genetic divergence, with the parameter including sequence percent identity, genetic distance, and length of monophyletic branch in a phylogenetic tree. The result showed that most samples had a high percentage of sequence identities, low genetic distances, and short chapters in monophyletic clades, but the remaining were not. Those data indicated that most samples could be identified at species-level without doubt and support conventional identification. Barcoding success is also depending on the availability of conspecific sequences in the databases. This study concluded that molecular barcoding could strengthen and validate traditional identification.
format	article
author	Nuryanto Agus Kusbiyanto Kusbiyanto Bhagawati Dian
author_facet	Nuryanto Agus Kusbiyanto Kusbiyanto Bhagawati Dian
author_sort	Nuryanto Agus
title	Molecular barcoding of marine ornamental fish from the southern coast of West Java validates conventional identification
title_short	Molecular barcoding of marine ornamental fish from the southern coast of West Java validates conventional identification
title_full	Molecular barcoding of marine ornamental fish from the southern coast of West Java validates conventional identification
title_fullStr	Molecular barcoding of marine ornamental fish from the southern coast of West Java validates conventional identification
title_full_unstemmed	Molecular barcoding of marine ornamental fish from the southern coast of West Java validates conventional identification
title_sort	molecular barcoding of marine ornamental fish from the southern coast of west java validates conventional identification
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/44a2b62dfd484c9092531df6f39b7f22
work_keys_str_mv	AT nuryantoagus molecularbarcodingofmarineornamentalfishfromthesoutherncoastofwestjavavalidatesconventionalidentification AT kusbiyantokusbiyanto molecularbarcodingofmarineornamentalfishfromthesoutherncoastofwestjavavalidatesconventionalidentification AT bhagawatidian molecularbarcodingofmarineornamentalfishfromthesoutherncoastofwestjavavalidatesconventionalidentification
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Molecular barcoding of marine ornamental fish from the southern coast of West Java validates conventional identification

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