Simultaneous Effect of Resveratrol and Probiotics on Insulin Resistance and Glucagon-Like Peptide (GLP-1) Levels in Diabetic Rats
BACKGROUND AND OBJECTIVE: The main and effective treatment for diabetes is the use of insulin and synthetic drugs and herbal medicines, which may be accompanied by several side effects. Given that GLP-1 secretion is reduced in diabetic patients compared to healthy individuals, the aim of this study...
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Babol University of Medical Sciences
2021
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oai:doaj.org-article:44a91cc8f7b84df4a1b1db05ae910d612021-11-29T07:40:49ZSimultaneous Effect of Resveratrol and Probiotics on Insulin Resistance and Glucagon-Like Peptide (GLP-1) Levels in Diabetic Rats1561-41072251-7170https://doaj.org/article/44a91cc8f7b84df4a1b1db05ae910d612021-03-01T00:00:00Zhttp://jbums.org/article-1-9682-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: The main and effective treatment for diabetes is the use of insulin and synthetic drugs and herbal medicines, which may be accompanied by several side effects. Given that GLP-1 secretion is reduced in diabetic patients compared to healthy individuals, the aim of this study was to determine the simultaneous effect of resveratrol and probiotics on insulin secretion and glucagon-like peptide (GLP-1) and to evaluate insulin resistance in diabetic rats. METHODS: In this experimental study, 40 adult male Wistar rats weighing approximately 250-300 g were used. Animals were randomly divided into 5 groups: control group, diabetic group (using 65 mg/kg streptozotocin and 110 mg/kg nicotinamide intraperitoneally), diabetic group treated with probiotics (50×109 bacteria/kg body wt−1 daily by dissolving in water), diabetic group treated with resveratrol (10 mg/kg by gavage method) and diabetic group treated with probiotics and resveratrol (co-administration group). After four weeks, the animals were sacrificed and pancreatic tissue and blood samples were isolated for histopathological tests and biochemical tests. Blood glucose, insulin resistance, pancreatic GLP-1 as well as pancreatic histopathology were assessed. FINDINGS: The co-administration group compared with the diabetic group caused a 42% reduction in serum glucose levels (p<0.001) and a 30% reduction in insulin resistance (p<0.001). The co-administration group also caused a 23.5% increase in insulin levels (p<0.001) and an 88% increase in GLP-1 (p<0.05). The co-administration group improved the histology of the pancreas. CONCLUSION: The results of the study showed that resveratrol and probiotics are effective in controlling diabetes by increasing the levels of GLP-1 and insulin and decreasing insulin resistance.A PegahI KhodadadiF MirzaeiH TayebiniaE AbbasiBabol University of Medical Sciencesarticleprobioticsresveratroltype 2 diabetesglucagon-like peptide (glp-1).MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 23, Iss 1, Pp 337-344 (2021) |
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EN FA |
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probiotics resveratrol type 2 diabetes glucagon-like peptide (glp-1). Medicine R Medicine (General) R5-920 |
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probiotics resveratrol type 2 diabetes glucagon-like peptide (glp-1). Medicine R Medicine (General) R5-920 A Pegah I Khodadadi F Mirzaei H Tayebinia E Abbasi Simultaneous Effect of Resveratrol and Probiotics on Insulin Resistance and Glucagon-Like Peptide (GLP-1) Levels in Diabetic Rats |
| description |
BACKGROUND AND OBJECTIVE: The main and effective treatment for diabetes is the use of insulin and synthetic drugs and herbal medicines, which may be accompanied by several side effects. Given that GLP-1 secretion is reduced in diabetic patients compared to healthy individuals, the aim of this study was to determine the simultaneous effect of resveratrol and probiotics on insulin secretion and glucagon-like peptide (GLP-1) and to evaluate insulin resistance in diabetic rats.
METHODS: In this experimental study, 40 adult male Wistar rats weighing approximately 250-300 g were used. Animals were randomly divided into 5 groups: control group, diabetic group (using 65 mg/kg streptozotocin and 110 mg/kg nicotinamide intraperitoneally), diabetic group treated with probiotics (50×109 bacteria/kg body wt−1 daily by dissolving in water), diabetic group treated with resveratrol (10 mg/kg by gavage method) and diabetic group treated with probiotics and resveratrol (co-administration group). After four weeks, the animals were sacrificed and pancreatic tissue and blood samples were isolated for histopathological tests and biochemical tests. Blood glucose, insulin resistance, pancreatic GLP-1 as well as pancreatic histopathology were assessed.
FINDINGS: The co-administration group compared with the diabetic group caused a 42% reduction in serum glucose levels (p<0.001) and a 30% reduction in insulin resistance (p<0.001). The co-administration group also caused a 23.5% increase in insulin levels (p<0.001) and an 88% increase in GLP-1 (p<0.05). The co-administration group improved the histology of the pancreas.
CONCLUSION: The results of the study showed that resveratrol and probiotics are effective in controlling diabetes by increasing the levels of GLP-1 and insulin and decreasing insulin resistance. |
| format |
article |
| author |
A Pegah I Khodadadi F Mirzaei H Tayebinia E Abbasi |
| author_facet |
A Pegah I Khodadadi F Mirzaei H Tayebinia E Abbasi |
| author_sort |
A Pegah |
| title |
Simultaneous Effect of Resveratrol and Probiotics on Insulin Resistance and Glucagon-Like Peptide (GLP-1) Levels in Diabetic Rats |
| title_short |
Simultaneous Effect of Resveratrol and Probiotics on Insulin Resistance and Glucagon-Like Peptide (GLP-1) Levels in Diabetic Rats |
| title_full |
Simultaneous Effect of Resveratrol and Probiotics on Insulin Resistance and Glucagon-Like Peptide (GLP-1) Levels in Diabetic Rats |
| title_fullStr |
Simultaneous Effect of Resveratrol and Probiotics on Insulin Resistance and Glucagon-Like Peptide (GLP-1) Levels in Diabetic Rats |
| title_full_unstemmed |
Simultaneous Effect of Resveratrol and Probiotics on Insulin Resistance and Glucagon-Like Peptide (GLP-1) Levels in Diabetic Rats |
| title_sort |
simultaneous effect of resveratrol and probiotics on insulin resistance and glucagon-like peptide (glp-1) levels in diabetic rats |
| publisher |
Babol University of Medical Sciences |
| publishDate |
2021 |
| url |
https://doaj.org/article/44a91cc8f7b84df4a1b1db05ae910d61 |
| work_keys_str_mv |
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