Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression
Tong Hao,1,2,* Jin Zhou,2,* Shuanghong Lü,3,* Boguang Yang,2,4 Yan Wang,2 Wancai Fang,2,4 Xiaoxia Jiang,2 Qiuxia Lin,2 Junjie Li,2 Changyong Wang1,21School of Life Science and Technology, Harbin Institute of Technology, Harbin, 2Department of Advanced Interdisciplinary Studies, Institute of...
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Dove Medical Press
2016
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oai:doaj.org-article:44b203df44394342a56787596a84cecf2021-12-02T02:01:31ZFullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression1178-2013https://doaj.org/article/44b203df44394342a56787596a84cecf2016-01-01T00:00:00Zhttps://www.dovepress.com/fullerene-mediates-proliferation-and-cardiomyogenic-differentiation-of-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Tong Hao,1,2,* Jin Zhou,2,* Shuanghong Lü,3,* Boguang Yang,2,4 Yan Wang,2 Wancai Fang,2,4 Xiaoxia Jiang,2 Qiuxia Lin,2 Junjie Li,2 Changyong Wang1,21School of Life Science and Technology, Harbin Institute of Technology, Harbin, 2Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, 3Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, 4Department of Polymer Science, Key Laboratory of Systems Bioengineering of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People’s Republic of China*These authors contributed equally to this workAbstract: Zero-dimensional fullerenes can modulate the biological behavior of a variety of cell lines. However, the effects and molecular mechanisms of proliferation and cardiomyogenic differentiation in brown adipose-derived stem cells (BADSCs) are still unclear. In this study, we report the initial biological effects of fullerene-C60 on BADSCs at different concentrations. Results suggest that fullerene-C60 has no cytotoxic effects on BADSCs even at a concentration of 100 µg/mL. Fullerene-C60 improves the MAPK expression level and stem cell survival, proliferation, and cardiomyogenesis. Further, we found that the fullerene-C60 modulates cardiomyogenic differentiation. Fullerene-C60 improves the expression of cardiomyocyte-specific proteins (cTnT and α-sarcomeric actinin). At elevated concentration, fullerene-C60 reduces the incidence of diminished spontaneous cardiac differentiation of BADSCs with time. At the genetic level, fullerene-C60 (5 µg/mL) also improves the expression of cTnT. In addition, fullerene-C60 promotes the formation of gap junction among cells. These findings have important implications for clinical application of fullerenes in the treatment of myocardial infarction.Keywords: C60, BADSCs, molecular mechanisms, myocardiumHao TZhou JLü SHYang BGWang YFang WCJiang XXLin QXLi JJWang CYDove Medical PressarticleC60BADSCsImprove growthMyocardiumMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 269-283 (2016) |
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C60 BADSCs Improve growth Myocardium Medicine (General) R5-920 |
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C60 BADSCs Improve growth Myocardium Medicine (General) R5-920 Hao T Zhou J Lü SH Yang BG Wang Y Fang WC Jiang XX Lin QX Li JJ Wang CY Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression |
| description |
Tong Hao,1,2,* Jin Zhou,2,* Shuanghong Lü,3,* Boguang Yang,2,4 Yan Wang,2 Wancai Fang,2,4 Xiaoxia Jiang,2 Qiuxia Lin,2 Junjie Li,2 Changyong Wang1,21School of Life Science and Technology, Harbin Institute of Technology, Harbin, 2Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, 3Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, 4Department of Polymer Science, Key Laboratory of Systems Bioengineering of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People’s Republic of China*These authors contributed equally to this workAbstract: Zero-dimensional fullerenes can modulate the biological behavior of a variety of cell lines. However, the effects and molecular mechanisms of proliferation and cardiomyogenic differentiation in brown adipose-derived stem cells (BADSCs) are still unclear. In this study, we report the initial biological effects of fullerene-C60 on BADSCs at different concentrations. Results suggest that fullerene-C60 has no cytotoxic effects on BADSCs even at a concentration of 100 µg/mL. Fullerene-C60 improves the MAPK expression level and stem cell survival, proliferation, and cardiomyogenesis. Further, we found that the fullerene-C60 modulates cardiomyogenic differentiation. Fullerene-C60 improves the expression of cardiomyocyte-specific proteins (cTnT and α-sarcomeric actinin). At elevated concentration, fullerene-C60 reduces the incidence of diminished spontaneous cardiac differentiation of BADSCs with time. At the genetic level, fullerene-C60 (5 µg/mL) also improves the expression of cTnT. In addition, fullerene-C60 promotes the formation of gap junction among cells. These findings have important implications for clinical application of fullerenes in the treatment of myocardial infarction.Keywords: C60, BADSCs, molecular mechanisms, myocardium |
| format |
article |
| author |
Hao T Zhou J Lü SH Yang BG Wang Y Fang WC Jiang XX Lin QX Li JJ Wang CY |
| author_facet |
Hao T Zhou J Lü SH Yang BG Wang Y Fang WC Jiang XX Lin QX Li JJ Wang CY |
| author_sort |
Hao T |
| title |
Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression |
| title_short |
Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression |
| title_full |
Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression |
| title_fullStr |
Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression |
| title_full_unstemmed |
Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression |
| title_sort |
fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of mapk pathway and cardiac protein expression |
| publisher |
Dove Medical Press |
| publishDate |
2016 |
| url |
https://doaj.org/article/44b203df44394342a56787596a84cecf |
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