Establishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme

Wenxin Zhang,1 Xin Lu,1 Zuoying Yuan,2 Minjuan Shen,1 Yunjia Song,1 Huanhuan Liu,1 Jingjing Deng,1 Xue Zhong,1 Xu Zhang1 1School of Dentistry, Stomatological Hospital, Tianjin Medical University, Tianjin, People’s Republic of China; 2State Key Laboratory of Organic-Inorganic Composites, B...

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Autores principales:	Zhang W, Lu X, Yuan Z, Shen M, Song Y, Liu H, Deng J, Zhong X, Zhang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	titanium surface modification aspirin osteoimmunomodulation osseointegration phase-transited lysozyme Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/44b874408b434a78b0a05b9d784f1397
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spelling	oai:doaj.org-article:44b874408b434a78b0a05b9d784f13972021-12-02T07:39:30ZEstablishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme1178-2013https://doaj.org/article/44b874408b434a78b0a05b9d784f13972019-02-01T00:00:00Zhttps://www.dovepress.com/establishing-an-osteoimmunomodulatory-coating-loaded-with-aspirin-on-t-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wenxin Zhang,1 Xin Lu,1 Zuoying Yuan,2 Minjuan Shen,1 Yunjia Song,1 Huanhuan Liu,1 Jingjing Deng,1 Xue Zhong,1 Xu Zhang1 1School of Dentistry, Stomatological Hospital, Tianjin Medical University, Tianjin, People’s Republic of China; 2State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, People’s Republic of China Background: To improve osseointegration and enhance the success rate of implanted biomaterials, the surface modification technology of bone implants has developed rapidly. Intensive research on osteoimmunomodulation has shown that the surfaces of implants should possess favorable osteoimmunomodulation to facilitate osteogenesis.Methods: A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge. In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.Results: The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages. Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification. After implantation in vivo, the ASA-modified implant can significantly contribute to bone formation around the implant, which mirrors the evaluation in vitro. Conclusion: This study highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant. Implant modification with ASA potentially provides superior strategies for the surface modification of biomaterials. Keywords: titanium surface modification, nanoparticle, osteoimmunomodulation, osseointegration  Zhang WLu XYuan ZShen MSong YLiu HDeng JZhong XZhang XDove Medical Pressarticletitanium surface modificationaspirinosteoimmunomodulationosseointegrationphase-transited lysozymeMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 977-991 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	titanium surface modification aspirin osteoimmunomodulation osseointegration phase-transited lysozyme Medicine (General) R5-920
spellingShingle	titanium surface modification aspirin osteoimmunomodulation osseointegration phase-transited lysozyme Medicine (General) R5-920 Zhang W Lu X Yuan Z Shen M Song Y Liu H Deng J Zhong X Zhang X Establishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme
description	Wenxin Zhang,1 Xin Lu,1 Zuoying Yuan,2 Minjuan Shen,1 Yunjia Song,1 Huanhuan Liu,1 Jingjing Deng,1 Xue Zhong,1 Xu Zhang1 1School of Dentistry, Stomatological Hospital, Tianjin Medical University, Tianjin, People’s Republic of China; 2State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, People’s Republic of China Background: To improve osseointegration and enhance the success rate of implanted biomaterials, the surface modification technology of bone implants has developed rapidly. Intensive research on osteoimmunomodulation has shown that the surfaces of implants should possess favorable osteoimmunomodulation to facilitate osteogenesis.Methods: A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge. In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.Results: The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages. Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification. After implantation in vivo, the ASA-modified implant can significantly contribute to bone formation around the implant, which mirrors the evaluation in vitro. Conclusion: This study highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant. Implant modification with ASA potentially provides superior strategies for the surface modification of biomaterials. Keywords: titanium surface modification, nanoparticle, osteoimmunomodulation, osseointegration
format	article
author	Zhang W Lu X Yuan Z Shen M Song Y Liu H Deng J Zhong X Zhang X
author_facet	Zhang W Lu X Yuan Z Shen M Song Y Liu H Deng J Zhong X Zhang X
author_sort	Zhang W
title	Establishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme
title_short	Establishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme
title_full	Establishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme
title_fullStr	Establishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme
title_full_unstemmed	Establishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme
title_sort	establishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/44b874408b434a78b0a05b9d784f1397
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Establishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme

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