Co-exposure of sodium arsenite and uranyl acetate differentially alters gene expression in CD3/CD28 activated CD4+ T-cells

Communities in the western region of the United States experience environmental exposure to metal mixtures from living in proximity to numerous unremediated abandoned uranium mines. Metals including arsenic and uranium co-occur in and around these sites at levels higher than the United States Enviro...

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Autores principales:	Jodi R. Schilz, Erica J. Dashner-Titus, Li Luo, Karen A. Simmons, Debra A. MacKenzie, Laurie G. Hudson
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Uranium Arsenic T-lymphocytes Mixture toxicology Toxicology. Poisons RA1190-1270
Acceso en línea:	https://doaj.org/article/44c8671c56b640f9b37012ae77a6ee2a
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spelling	oai:doaj.org-article:44c8671c56b640f9b37012ae77a6ee2a2021-12-04T04:34:22ZCo-exposure of sodium arsenite and uranyl acetate differentially alters gene expression in CD3/CD28 activated CD4+ T-cells2214-750010.1016/j.toxrep.2021.11.019https://doaj.org/article/44c8671c56b640f9b37012ae77a6ee2a2021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2214750021002031https://doaj.org/toc/2214-7500Communities in the western region of the United States experience environmental exposure to metal mixtures from living in proximity to numerous unremediated abandoned uranium mines. Metals including arsenic and uranium co-occur in and around these sites at levels higher than the United States Environmental Protection Agency maximum contaminant levels. To address the potential effect of these metals on the activation of CD4+ T-cells, we used RNA sequencing methods to determine the effect of exposure to sodium arsenite (1 μM and 10 μM), uranyl acetate (3 μM and 30 μM) or a mixture of sodium arsenite and uranyl acetate (1 μM sodium arsenite + 3 μM uranyl acetate). Sodium arsenite induced a dose dependent effect on activation associated gene expression; targeting immune response genes at the lower dose. Increases in oxidative stress gene expression were observed with both sodium arsenite doses. While uranyl acetate alone did not significantly alter activation associated gene expression, the mixture of uranyl acetate with sodium arsenite demonstrated a combined effect relative to sodium arsenite alone. The results demonstrate the need to investigate metal and metalloid mixtures at environmentally relevant concentrations to better understand the toxicological impact of these mixtures on T-cell activation, function and immune dysregulation.Jodi R. SchilzErica J. Dashner-TitusLi LuoKaren A. SimmonsDebra A. MacKenzieLaurie G. HudsonElsevierarticleUraniumArsenicT-lymphocytesMixture toxicologyToxicology. PoisonsRA1190-1270ENToxicology Reports, Vol 8, Iss , Pp 1917-1929 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Uranium Arsenic T-lymphocytes Mixture toxicology Toxicology. Poisons RA1190-1270
spellingShingle	Uranium Arsenic T-lymphocytes Mixture toxicology Toxicology. Poisons RA1190-1270 Jodi R. Schilz Erica J. Dashner-Titus Li Luo Karen A. Simmons Debra A. MacKenzie Laurie G. Hudson Co-exposure of sodium arsenite and uranyl acetate differentially alters gene expression in CD3/CD28 activated CD4+ T-cells
description	Communities in the western region of the United States experience environmental exposure to metal mixtures from living in proximity to numerous unremediated abandoned uranium mines. Metals including arsenic and uranium co-occur in and around these sites at levels higher than the United States Environmental Protection Agency maximum contaminant levels. To address the potential effect of these metals on the activation of CD4+ T-cells, we used RNA sequencing methods to determine the effect of exposure to sodium arsenite (1 μM and 10 μM), uranyl acetate (3 μM and 30 μM) or a mixture of sodium arsenite and uranyl acetate (1 μM sodium arsenite + 3 μM uranyl acetate). Sodium arsenite induced a dose dependent effect on activation associated gene expression; targeting immune response genes at the lower dose. Increases in oxidative stress gene expression were observed with both sodium arsenite doses. While uranyl acetate alone did not significantly alter activation associated gene expression, the mixture of uranyl acetate with sodium arsenite demonstrated a combined effect relative to sodium arsenite alone. The results demonstrate the need to investigate metal and metalloid mixtures at environmentally relevant concentrations to better understand the toxicological impact of these mixtures on T-cell activation, function and immune dysregulation.
format	article
author	Jodi R. Schilz Erica J. Dashner-Titus Li Luo Karen A. Simmons Debra A. MacKenzie Laurie G. Hudson
author_facet	Jodi R. Schilz Erica J. Dashner-Titus Li Luo Karen A. Simmons Debra A. MacKenzie Laurie G. Hudson
author_sort	Jodi R. Schilz
title	Co-exposure of sodium arsenite and uranyl acetate differentially alters gene expression in CD3/CD28 activated CD4+ T-cells
title_short	Co-exposure of sodium arsenite and uranyl acetate differentially alters gene expression in CD3/CD28 activated CD4+ T-cells
title_full	Co-exposure of sodium arsenite and uranyl acetate differentially alters gene expression in CD3/CD28 activated CD4+ T-cells
title_fullStr	Co-exposure of sodium arsenite and uranyl acetate differentially alters gene expression in CD3/CD28 activated CD4+ T-cells
title_full_unstemmed	Co-exposure of sodium arsenite and uranyl acetate differentially alters gene expression in CD3/CD28 activated CD4+ T-cells
title_sort	co-exposure of sodium arsenite and uranyl acetate differentially alters gene expression in cd3/cd28 activated cd4+ t-cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/44c8671c56b640f9b37012ae77a6ee2a
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Co-exposure of sodium arsenite and uranyl acetate differentially alters gene expression in CD3/CD28 activated CD4+ T-cells

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