HO-1 protects the nerves of rats with cerebral hemorrhage by regulating the PI3K/AKT signaling pathway

Qingping Zhao,1,* Rongbo Qu,2,* Lu Teng,1 Changyou Yin,1 Yuan Yuan11Department of Neurosurgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai City, Shandong Province 264000, People’s Republic of China; 2Department of Neurosurgery, Yantai Affiliated Hospital of Bi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhao Q, Qu R, Teng L, Yin C, Yuan Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	PI3K/AKT signaling pathway HO-1 Neuroprotection；Hemorrhage Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/44ca6c3261224d9d8c1f807355048136
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:44ca6c3261224d9d8c1f807355048136
record_format	dspace
spelling	oai:doaj.org-article:44ca6c3261224d9d8c1f8073550481362021-12-02T10:11:52ZHO-1 protects the nerves of rats with cerebral hemorrhage by regulating the PI3K/AKT signaling pathway1178-2021https://doaj.org/article/44ca6c3261224d9d8c1f8073550481362019-05-01T00:00:00Zhttps://www.dovepress.com/ho-1-protects-the-nerves-of-rats-with-cerebral-hemorrhage-by-regulatin-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Qingping Zhao,1,* Rongbo Qu,2,* Lu Teng,1 Changyou Yin,1 Yuan Yuan11Department of Neurosurgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai City, Shandong Province 264000, People’s Republic of China; 2Department of Neurosurgery, Yantai Affiliated Hospital of Binzhou Medical University, Yantai City, Shandong Province 264100, People’s Republic of China *These authors contributed equally to this workObjective: This study aimed to investigate the neuroprotective effect of heme oxygenase-1 (HO-1) on the PI3K/AKT signaling pathway in rats with cerebral hemorrhage.Materials and methods: Adult male Sprague-Dawley rats were randomly divided into: a sham group, a model group and an HO-1 inhibitor group (ZnPP group). Functional defects after surgery were scored according to the Longa5 standard. Hemotoxylin and eosin staining was used to detect whether the model was constructed successfully. Superoxide dismutase (SOD) vitality and malondialdehyde (MDA) content were calculated by the xanthine oxidase method and thiobarbituric acid method, respectively. Blood-brain barrier permeability was measured by Evans Blue. Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay. The expression of Bcl-2 and BAX was evaluated by immunohistochemistry and the expression of PI3K, p-PI3K, AKT and p-AKT was tested by Western blotting.Results: The rat intracerebral hemorrhage model was successfully constructed. Compared with the model group, the bleeding in the ZnPP group was more serious, the cell edema and deformation were aggravated, and the neurological deficit score in the rat was significantly increased. In addition, the content of Evans blue, MDA, the number of apoptotic cells, the water content of brain tissue and the expression of BAX were significantly increased, while the SOD activity and the expressions of Bcl-2, p-PI3K and p-AKT protein were decreased.Conclusion: HO-1 could protect the nerves of rats with cerebral hemorrhage by regulating the PI3K/AKT signaling pathway.Keywords: PI3K/AKT signaling pathway, HO-1, neuroprotection, hemorrhageZhao QQu RTeng LYin CYuan YDove Medical PressarticlePI3K/AKT signaling pathwayHO-1Neuroprotection；HemorrhageNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 1459-1468 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	PI3K/AKT signaling pathway HO-1 Neuroprotection；Hemorrhage Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	PI3K/AKT signaling pathway HO-1 Neuroprotection；Hemorrhage Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Zhao Q Qu R Teng L Yin C Yuan Y HO-1 protects the nerves of rats with cerebral hemorrhage by regulating the PI3K/AKT signaling pathway
description	Qingping Zhao,1,* Rongbo Qu,2,* Lu Teng,1 Changyou Yin,1 Yuan Yuan11Department of Neurosurgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai City, Shandong Province 264000, People’s Republic of China; 2Department of Neurosurgery, Yantai Affiliated Hospital of Binzhou Medical University, Yantai City, Shandong Province 264100, People’s Republic of China *These authors contributed equally to this workObjective: This study aimed to investigate the neuroprotective effect of heme oxygenase-1 (HO-1) on the PI3K/AKT signaling pathway in rats with cerebral hemorrhage.Materials and methods: Adult male Sprague-Dawley rats were randomly divided into: a sham group, a model group and an HO-1 inhibitor group (ZnPP group). Functional defects after surgery were scored according to the Longa5 standard. Hemotoxylin and eosin staining was used to detect whether the model was constructed successfully. Superoxide dismutase (SOD) vitality and malondialdehyde (MDA) content were calculated by the xanthine oxidase method and thiobarbituric acid method, respectively. Blood-brain barrier permeability was measured by Evans Blue. Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay. The expression of Bcl-2 and BAX was evaluated by immunohistochemistry and the expression of PI3K, p-PI3K, AKT and p-AKT was tested by Western blotting.Results: The rat intracerebral hemorrhage model was successfully constructed. Compared with the model group, the bleeding in the ZnPP group was more serious, the cell edema and deformation were aggravated, and the neurological deficit score in the rat was significantly increased. In addition, the content of Evans blue, MDA, the number of apoptotic cells, the water content of brain tissue and the expression of BAX were significantly increased, while the SOD activity and the expressions of Bcl-2, p-PI3K and p-AKT protein were decreased.Conclusion: HO-1 could protect the nerves of rats with cerebral hemorrhage by regulating the PI3K/AKT signaling pathway.Keywords: PI3K/AKT signaling pathway, HO-1, neuroprotection, hemorrhage
format	article
author	Zhao Q Qu R Teng L Yin C Yuan Y
author_facet	Zhao Q Qu R Teng L Yin C Yuan Y
author_sort	Zhao Q
title	HO-1 protects the nerves of rats with cerebral hemorrhage by regulating the PI3K/AKT signaling pathway
title_short	HO-1 protects the nerves of rats with cerebral hemorrhage by regulating the PI3K/AKT signaling pathway
title_full	HO-1 protects the nerves of rats with cerebral hemorrhage by regulating the PI3K/AKT signaling pathway
title_fullStr	HO-1 protects the nerves of rats with cerebral hemorrhage by regulating the PI3K/AKT signaling pathway
title_full_unstemmed	HO-1 protects the nerves of rats with cerebral hemorrhage by regulating the PI3K/AKT signaling pathway
title_sort	ho-1 protects the nerves of rats with cerebral hemorrhage by regulating the pi3k/akt signaling pathway
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/44ca6c3261224d9d8c1f807355048136
work_keys_str_mv	AT zhaoq ho1protectsthenervesofratswithcerebralhemorrhagebyregulatingthepi3kaktsignalingpathway AT qur ho1protectsthenervesofratswithcerebralhemorrhagebyregulatingthepi3kaktsignalingpathway AT tengl ho1protectsthenervesofratswithcerebralhemorrhagebyregulatingthepi3kaktsignalingpathway AT yinc ho1protectsthenervesofratswithcerebralhemorrhagebyregulatingthepi3kaktsignalingpathway AT yuany ho1protectsthenervesofratswithcerebralhemorrhagebyregulatingthepi3kaktsignalingpathway
_version_	1718397519655862272

HO-1 protects the nerves of rats with cerebral hemorrhage by regulating the PI3K/AKT signaling pathway

Ejemplares similares