A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion

A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion

It is challenging to quantify anisotropic diffusion in biological systems. Here the authors report light-sheet imaging-based Fourier transform fluorescence recovery after photobleaching (LiFT-FRAP) to noninvasively determine 3D diffusion tensors of various biomolecules at physiological diffusivity.

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Autores principales: Peng Chen, Xun Chen, R. Glenn Hepfer, Brooke J. Damon, Changcheng Shi, Jenny J. Yao, Matthew C. Coombs, Michael J. Kern, Tong Ye, Hai Yao
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/44ccdadf5df14f948c667b4abb8d9399
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spelling oai:doaj.org-article:44ccdadf5df14f948c667b4abb8d93992021-12-02T17:04:30ZA noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion10.1038/s41467-021-22221-02041-1723https://doaj.org/article/44ccdadf5df14f948c667b4abb8d93992021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22221-0https://doaj.org/toc/2041-1723It is challenging to quantify anisotropic diffusion in biological systems. Here the authors report light-sheet imaging-based Fourier transform fluorescence recovery after photobleaching (LiFT-FRAP) to noninvasively determine 3D diffusion tensors of various biomolecules at physiological diffusivity.Peng ChenXun ChenR. Glenn HepferBrooke J. DamonChangcheng ShiJenny J. YaoMatthew C. CoombsMichael J. KernTong YeHai YaoNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Peng Chen
Xun Chen
R. Glenn Hepfer
Brooke J. Damon
Changcheng Shi
Jenny J. Yao
Matthew C. Coombs
Michael J. Kern
Tong Ye
Hai Yao
A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion
description It is challenging to quantify anisotropic diffusion in biological systems. Here the authors report light-sheet imaging-based Fourier transform fluorescence recovery after photobleaching (LiFT-FRAP) to noninvasively determine 3D diffusion tensors of various biomolecules at physiological diffusivity.
format article
author Peng Chen
Xun Chen
R. Glenn Hepfer
Brooke J. Damon
Changcheng Shi
Jenny J. Yao
Matthew C. Coombs
Michael J. Kern
Tong Ye
Hai Yao
author_facet Peng Chen
Xun Chen
R. Glenn Hepfer
Brooke J. Damon
Changcheng Shi
Jenny J. Yao
Matthew C. Coombs
Michael J. Kern
Tong Ye
Hai Yao
author_sort Peng Chen
title A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion
title_short A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion
title_full A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion
title_fullStr A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion
title_full_unstemmed A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion
title_sort noninvasive fluorescence imaging-based platform measures 3d anisotropic extracellular diffusion
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/44ccdadf5df14f948c667b4abb8d9399
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