A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion

It is challenging to quantify anisotropic diffusion in biological systems. Here the authors report light-sheet imaging-based Fourier transform fluorescence recovery after photobleaching (LiFT-FRAP) to noninvasively determine 3D diffusion tensors of various biomolecules at physiological diffusivity.

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Detalles Bibliográficos
Autores principales: Peng Chen, Xun Chen, R. Glenn Hepfer, Brooke J. Damon, Changcheng Shi, Jenny J. Yao, Matthew C. Coombs, Michael J. Kern, Tong Ye, Hai Yao
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/44ccdadf5df14f948c667b4abb8d9399
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