The synthetic melanocortin (CKPV)2 exerts anti-fungal and anti-inflammatory effects against Candida albicans vaginitis via inducing macrophage M2 polarization.

In this study, we examined anti-fungal and anti-inflammatory effects of the synthetic melanocortin peptide (Ac-Cys-Lys-Pro-Val-NH2)2 or (CKPV)2 against Candida albicans vaginitis. Our in vitro results showed that (CKPV)2 dose-dependently inhibited Candida albicans colonies formation. In a rat Candid...

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Autores principales:	Hai-xia Ji, Yu-lian Zou, Jing-jing Duan, Zhi-rong Jia, Xian-jing Li, Zhuo Wang, Li Li, Yong-wen Li, Gen-yan Liu, Ming-qing Tong, Xiao-yi Li, Guo-hui Zhang, Xiang-rong Dai, Ling He, Zhi-yu Li, Cong Cao, Yong Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/44cea16732ee498fbc6d51c6cef069d7
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spelling	oai:doaj.org-article:44cea16732ee498fbc6d51c6cef069d72021-11-18T07:57:30ZThe synthetic melanocortin (CKPV)2 exerts anti-fungal and anti-inflammatory effects against Candida albicans vaginitis via inducing macrophage M2 polarization.1932-620310.1371/journal.pone.0056004https://doaj.org/article/44cea16732ee498fbc6d51c6cef069d72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23457491/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In this study, we examined anti-fungal and anti-inflammatory effects of the synthetic melanocortin peptide (Ac-Cys-Lys-Pro-Val-NH2)2 or (CKPV)2 against Candida albicans vaginitis. Our in vitro results showed that (CKPV)2 dose-dependently inhibited Candida albicans colonies formation. In a rat Candida albicans vaginitis model, (CKPV)2 significantly inhibited vaginal Candida albicans survival and macrophages sub-epithelial mucosa infiltration. For mechanisms study, we observed that (CKPV)2 inhibited macrophages phagocytosis of Candida albicans. Meanwhile, (CKPV)2 administration inhibited macrophage pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) release, while increasing the arginase activity and anti-inflammatory cytokine IL-10 production, suggesting macrophages M1 to M2 polarization. Cyclic AMP (cAMP) production was also induced by (CKPV)2 administration in macrophages. These above effects on macrophages by (CKPV)2 were almost reversed by melanocortin receptor-1(MC1R) siRNA knockdown, indicating the requirement of MC1R in the process. Altogether, our results suggest that (CKPV)2 exerted anti-fungal and anti-inflammatory activities against Candida albicans vaginitis probably through inducing macrophages M1 to M2 polarization and MC1R activation.Hai-xia JiYu-lian ZouJing-jing DuanZhi-rong JiaXian-jing LiZhuo WangLi LiYong-wen LiGen-yan LiuMing-qing TongXiao-yi LiGuo-hui ZhangXiang-rong DaiLing HeZhi-yu LiCong CaoYong YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e56004 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hai-xia Ji Yu-lian Zou Jing-jing Duan Zhi-rong Jia Xian-jing Li Zhuo Wang Li Li Yong-wen Li Gen-yan Liu Ming-qing Tong Xiao-yi Li Guo-hui Zhang Xiang-rong Dai Ling He Zhi-yu Li Cong Cao Yong Yang The synthetic melanocortin (CKPV)2 exerts anti-fungal and anti-inflammatory effects against Candida albicans vaginitis via inducing macrophage M2 polarization.
description	In this study, we examined anti-fungal and anti-inflammatory effects of the synthetic melanocortin peptide (Ac-Cys-Lys-Pro-Val-NH2)2 or (CKPV)2 against Candida albicans vaginitis. Our in vitro results showed that (CKPV)2 dose-dependently inhibited Candida albicans colonies formation. In a rat Candida albicans vaginitis model, (CKPV)2 significantly inhibited vaginal Candida albicans survival and macrophages sub-epithelial mucosa infiltration. For mechanisms study, we observed that (CKPV)2 inhibited macrophages phagocytosis of Candida albicans. Meanwhile, (CKPV)2 administration inhibited macrophage pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) release, while increasing the arginase activity and anti-inflammatory cytokine IL-10 production, suggesting macrophages M1 to M2 polarization. Cyclic AMP (cAMP) production was also induced by (CKPV)2 administration in macrophages. These above effects on macrophages by (CKPV)2 were almost reversed by melanocortin receptor-1(MC1R) siRNA knockdown, indicating the requirement of MC1R in the process. Altogether, our results suggest that (CKPV)2 exerted anti-fungal and anti-inflammatory activities against Candida albicans vaginitis probably through inducing macrophages M1 to M2 polarization and MC1R activation.
format	article
author	Hai-xia Ji Yu-lian Zou Jing-jing Duan Zhi-rong Jia Xian-jing Li Zhuo Wang Li Li Yong-wen Li Gen-yan Liu Ming-qing Tong Xiao-yi Li Guo-hui Zhang Xiang-rong Dai Ling He Zhi-yu Li Cong Cao Yong Yang
author_facet	Hai-xia Ji Yu-lian Zou Jing-jing Duan Zhi-rong Jia Xian-jing Li Zhuo Wang Li Li Yong-wen Li Gen-yan Liu Ming-qing Tong Xiao-yi Li Guo-hui Zhang Xiang-rong Dai Ling He Zhi-yu Li Cong Cao Yong Yang
author_sort	Hai-xia Ji
title	The synthetic melanocortin (CKPV)2 exerts anti-fungal and anti-inflammatory effects against Candida albicans vaginitis via inducing macrophage M2 polarization.
title_short	The synthetic melanocortin (CKPV)2 exerts anti-fungal and anti-inflammatory effects against Candida albicans vaginitis via inducing macrophage M2 polarization.
title_full	The synthetic melanocortin (CKPV)2 exerts anti-fungal and anti-inflammatory effects against Candida albicans vaginitis via inducing macrophage M2 polarization.
title_fullStr	The synthetic melanocortin (CKPV)2 exerts anti-fungal and anti-inflammatory effects against Candida albicans vaginitis via inducing macrophage M2 polarization.
title_full_unstemmed	The synthetic melanocortin (CKPV)2 exerts anti-fungal and anti-inflammatory effects against Candida albicans vaginitis via inducing macrophage M2 polarization.
title_sort	synthetic melanocortin (ckpv)2 exerts anti-fungal and anti-inflammatory effects against candida albicans vaginitis via inducing macrophage m2 polarization.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/44cea16732ee498fbc6d51c6cef069d7
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The synthetic melanocortin (CKPV)2 exerts anti-fungal and anti-inflammatory effects against Candida albicans vaginitis via inducing macrophage M2 polarization.

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