Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume

Hoya multiflora Blume has been used for various purposes. Extract hoya has been reported to have bioactivity but extraction from the plant has been inefficient because it requires large biomass. Therefore, one of the appropriate ways to extract its bioactive compounds is using endophytic bacteria. T...

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Autores principales:	Dewinta Nur Alvionita, SRI RAHAYU, NISA RACHMANIA MUBARIK
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	16s rrna, antimicrobial, bioactive compound, endophytic bacteria, mic Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/44d1c29a60e648b48ba5a47ebe60ebe8
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spelling	oai:doaj.org-article:44d1c29a60e648b48ba5a47ebe60ebe82021-11-21T22:09:24ZCharacterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume1412-033X2085-472210.13057/biodiv/d210125https://doaj.org/article/44d1c29a60e648b48ba5a47ebe60ebe82019-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4511https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Hoya multiflora Blume has been used for various purposes. Extract hoya has been reported to have bioactivity but extraction from the plant has been inefficient because it requires large biomass. Therefore, one of the appropriate ways to extract its bioactive compounds is using endophytic bacteria. The purposes of this research were to isolate, characterize, test its antimicrobial activity against two pathogenic bacteria and identify its bioactive compound. A total of 18 isolates from H. multiflora Blume were successfully obtained. Based on antimicrobial test, one isolate was able to inhibit Escherichia coli, one isolate inhibited Staphylococcus aureus and two isolates inhibited both of pathogenic bacteria. In addition, minimum inhibitory concentration (MIC) of ethyl acetate extract from HMB 1 was 62.5 ppm, while from HMD 4 was 125 ppm, both against E. coli and S. aureus. Based on 16S rRNA gene sequencing analysis, HMB 1 was the most similar (99.91%) with Bacillus siamensis KCTC-13613 and HMD 4 was the most similar (100%) with Bacillus aryabhattai B82W22. Using GC-MS, pyrollo[1,2-a]-pyrazine-1,4-dione was found to be dominant compound in the exctract bacteria of HMB 1and HMD 4 while Stigmasterol was found in extract HMD 4 and extract leaves H. multiflora Blume, respectively.Dewinta Nur AlvionitaSRI RAHAYUNISA RACHMANIA MUBARIKMBI & UNS Soloarticle16s rrna, antimicrobial, bioactive compound, endophytic bacteria, micBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna, antimicrobial, bioactive compound, endophytic bacteria, mic Biology (General) QH301-705.5
spellingShingle	16s rrna, antimicrobial, bioactive compound, endophytic bacteria, mic Biology (General) QH301-705.5 Dewinta Nur Alvionita SRI RAHAYU NISA RACHMANIA MUBARIK Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume
description	Hoya multiflora Blume has been used for various purposes. Extract hoya has been reported to have bioactivity but extraction from the plant has been inefficient because it requires large biomass. Therefore, one of the appropriate ways to extract its bioactive compounds is using endophytic bacteria. The purposes of this research were to isolate, characterize, test its antimicrobial activity against two pathogenic bacteria and identify its bioactive compound. A total of 18 isolates from H. multiflora Blume were successfully obtained. Based on antimicrobial test, one isolate was able to inhibit Escherichia coli, one isolate inhibited Staphylococcus aureus and two isolates inhibited both of pathogenic bacteria. In addition, minimum inhibitory concentration (MIC) of ethyl acetate extract from HMB 1 was 62.5 ppm, while from HMD 4 was 125 ppm, both against E. coli and S. aureus. Based on 16S rRNA gene sequencing analysis, HMB 1 was the most similar (99.91%) with Bacillus siamensis KCTC-13613 and HMD 4 was the most similar (100%) with Bacillus aryabhattai B82W22. Using GC-MS, pyrollo[1,2-a]-pyrazine-1,4-dione was found to be dominant compound in the exctract bacteria of HMB 1and HMD 4 while Stigmasterol was found in extract HMD 4 and extract leaves H. multiflora Blume, respectively.
format	article
author	Dewinta Nur Alvionita SRI RAHAYU NISA RACHMANIA MUBARIK
author_facet	Dewinta Nur Alvionita SRI RAHAYU NISA RACHMANIA MUBARIK
author_sort	Dewinta Nur Alvionita
title	Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume
title_short	Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume
title_full	Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume
title_fullStr	Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume
title_full_unstemmed	Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume
title_sort	characterization, identification, and analysis of bioactive compound of endophytic bacteria from hoya multiflora blume
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/44d1c29a60e648b48ba5a47ebe60ebe8
work_keys_str_mv	AT dewintanuralvionita characterizationidentificationandanalysisofbioactivecompoundofendophyticbacteriafromhoyamultiflorablume AT srirahayu characterizationidentificationandanalysisofbioactivecompoundofendophyticbacteriafromhoyamultiflorablume AT nisarachmaniamubarik characterizationidentificationandanalysisofbioactivecompoundofendophyticbacteriafromhoyamultiflorablume
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Characterization, identification, and analysis of bioactive compound of endophytic bacteria from Hoya multiflora Blume

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