MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION

The paper presents the development of an analytical method to determine dopamine by coupling reaction with diazotized solution of 4-Aminoantipyrine in basic media to give a compound with a single azo dye salt having orange color soluble in water. A calibration curve for a range of concentration (9....

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Autor principal:	AHMED JASSIM MUKLIVE AL-OGAIDI
Formato:	article
Lenguaje:	EN
Publicado:	Alma Mater Publishing House "Vasile Alecsandri" University of Bacau 2016
Materias:	dopamine coupling reaction biological samples azo dyes spectrophotometric Technology T Engineering (General). Civil engineering (General) TA1-2040
Acceso en línea:	https://doaj.org/article/44d7394ab1774f99ac902391eb76d974
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spelling	oai:doaj.org-article:44d7394ab1774f99ac902391eb76d9742021-12-02T18:36:10ZMICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION10.29081/jesr.v22i4.922068-75592344-4932https://doaj.org/article/44d7394ab1774f99ac902391eb76d9742016-12-01T00:00:00Zhttp://www.jesr.ub.ro/1/article/view/92https://doaj.org/toc/2068-7559https://doaj.org/toc/2344-4932 The paper presents the development of an analytical method to determine dopamine by coupling reaction with diazotized solution of 4-Aminoantipyrine in basic media to give a compound with a single azo dye salt having orange color soluble in water. A calibration curve for a range of concentration (9.14x10-5 – 2.28x10-3 μg.mL-1) was realized and the value of molar absortivity was 1.5x103 L.mol-1.cm-1, with a relative standard deviation more than 1.26% and a recovery 98.77%. As we study the nature of the azo dye by mole fraction method, from the practical value we found that the mole fraction of the dye compound is 1:1 (dopamine: 4- amino antipyrine) and the stability factor reach to 1.4x106 L.mol-1. The described procedure is very simple, low-time-consuming, provides high throughput of examined samples, and could be used for routine screening and confirmatory analyses as well. The method was successfully validated to the analysis of the dopamine in biological samples. AHMED JASSIM MUKLIVE AL-OGAIDI Alma Mater Publishing House "Vasile Alecsandri" University of Bacauarticledopaminecoupling reactionbiological samplesazo dyesspectrophotometricTechnologyTEngineering (General). Civil engineering (General)TA1-2040ENJournal of Engineering Studies and Research, Vol 22, Iss 4 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	dopamine coupling reaction biological samples azo dyes spectrophotometric Technology T Engineering (General). Civil engineering (General) TA1-2040
spellingShingle	dopamine coupling reaction biological samples azo dyes spectrophotometric Technology T Engineering (General). Civil engineering (General) TA1-2040 AHMED JASSIM MUKLIVE AL-OGAIDI MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION
description	The paper presents the development of an analytical method to determine dopamine by coupling reaction with diazotized solution of 4-Aminoantipyrine in basic media to give a compound with a single azo dye salt having orange color soluble in water. A calibration curve for a range of concentration (9.14x10-5 – 2.28x10-3 μg.mL-1) was realized and the value of molar absortivity was 1.5x103 L.mol-1.cm-1, with a relative standard deviation more than 1.26% and a recovery 98.77%. As we study the nature of the azo dye by mole fraction method, from the practical value we found that the mole fraction of the dye compound is 1:1 (dopamine: 4- amino antipyrine) and the stability factor reach to 1.4x106 L.mol-1. The described procedure is very simple, low-time-consuming, provides high throughput of examined samples, and could be used for routine screening and confirmatory analyses as well. The method was successfully validated to the analysis of the dopamine in biological samples.
format	article
author	AHMED JASSIM MUKLIVE AL-OGAIDI
author_facet	AHMED JASSIM MUKLIVE AL-OGAIDI
author_sort	AHMED JASSIM MUKLIVE AL-OGAIDI
title	MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION
title_short	MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION
title_full	MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION
title_fullStr	MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION
title_full_unstemmed	MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION
title_sort	micro determination of dopamine in biological samples by coupling reaction
publisher	Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
publishDate	2016
url	https://doaj.org/article/44d7394ab1774f99ac902391eb76d974
work_keys_str_mv	AT ahmedjassimmuklivealogaidi microdeterminationofdopamineinbiologicalsamplesbycouplingreaction
_version_	1718377855313772544

MICRO DETERMINATION OF DOPAMINE IN BIOLOGICAL SAMPLES BY COUPLING REACTION

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