IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated <named-content content-type="genus-species">Acinetobacter baumannii</named-content> GC1 and GC2 Clones

ABSTRACT Clear similarities between antibiotic resistance islands in the chromosomes of extensively antibiotic-resistant isolates from the two dominant, globally distributed Acinetobacter baumannii clones, GC1 and GC2, suggest a common origin. A close relative of the likely progenitor of both of the...

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Autores principales:	Grace A. Blackwell, Mohammad Hamidian, Ruth M. Hall
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Acinetobacter baumannii genomic resistance islands IS26 IncM plasmid Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/44d9527e399146f2a138ed01c1d5349e
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spelling	oai:doaj.org-article:44d9527e399146f2a138ed01c1d5349e2021-11-15T15:21:18ZIncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated <named-content content-type="genus-species">Acinetobacter baumannii</named-content> GC1 and GC2 Clones10.1128/mSphere.00117-162379-5042https://doaj.org/article/44d9527e399146f2a138ed01c1d5349e2016-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00117-16https://doaj.org/toc/2379-5042ABSTRACT Clear similarities between antibiotic resistance islands in the chromosomes of extensively antibiotic-resistant isolates from the two dominant, globally distributed Acinetobacter baumannii clones, GC1 and GC2, suggest a common origin. A close relative of the likely progenitor of both of these regions was found in R1215, a conjugative IncM plasmid from a Serratia marcescens strain isolated prior to 1980. The 37.8-kb resistance region in R1215 lies within the mucB gene and includes aacC1, aadA1, aphA1b, blaTEM, catA1, sul1, and tetA(A), genes that confer resistance to gentamicin, streptomycin and spectinomycin, kanamycin and neomycin, ampicillin, chloramphenicol, sulfamethoxazole, and tetracycline, respectively. The backbone of this region is derived from Tn1721 and is interrupted by a hybrid Tn2670 (Tn21)-Tn1696-type transposon, Tn6020, and an incomplete Tn1. After minor rearrangements, this R1215 resistance island can generate AbGRI2-0, the predicted earliest form of the IS26-bounded AbGRI2-type resistance island of GC2 isolates, and to the multiple antibiotic resistance region (MARR) of AbaR0, the precursor of this region in AbaR-type resistance islands in the GC1 group. A 29.9-kb circle excised by IS26 has been inserted into the A. baumannii chromosome to generate AbGRI2-0. To create the MARR of AbaR0, a different circular form, again generated by IS26 from an R1215 resistance region variant, has been opened at a different point by recombination with a copy of the sul1 gene already present in the AbaR precursor. Recent IncM plasmids related to R1215 have a variant resistance island containing a blaSHV gene in the same location. IMPORTANCE Two lineages of extensively antibiotic-resistant A. baumannii currently plaguing modern medicine each acquired resistance to all of the original antibiotics (ampicillin, tetracycline, kanamycin, and sulfonamides) by the end of the 1970s and then became resistant to antibiotics from newer families after they were introduced in the 1980s. Here, we show that, in both of the dominant globally disseminated A. baumannii clones, a related set of antibiotic resistance genes was acquired together from the same resistance region that had already evolved in an IncM plasmid. In both cases, the action of IS26 was important in this process, but homologous recombination was also involved. The findings highlight the fact that complex regions carrying several resistance genes can evolve in one location or organism and all or part of the evolved region can then move to other locations and other organisms, conferring resistance to several antibiotics in a single step.Grace A. BlackwellMohammad HamidianRuth M. HallAmerican Society for MicrobiologyarticleAcinetobacter baumanniigenomic resistance islandsIS26IncM plasmidMicrobiologyQR1-502ENmSphere, Vol 1, Iss 3 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acinetobacter baumannii genomic resistance islands IS26 IncM plasmid Microbiology QR1-502
spellingShingle	Acinetobacter baumannii genomic resistance islands IS26 IncM plasmid Microbiology QR1-502 Grace A. Blackwell Mohammad Hamidian Ruth M. Hall IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated <named-content content-type="genus-species">Acinetobacter baumannii</named-content> GC1 and GC2 Clones
description	ABSTRACT Clear similarities between antibiotic resistance islands in the chromosomes of extensively antibiotic-resistant isolates from the two dominant, globally distributed Acinetobacter baumannii clones, GC1 and GC2, suggest a common origin. A close relative of the likely progenitor of both of these regions was found in R1215, a conjugative IncM plasmid from a Serratia marcescens strain isolated prior to 1980. The 37.8-kb resistance region in R1215 lies within the mucB gene and includes aacC1, aadA1, aphA1b, blaTEM, catA1, sul1, and tetA(A), genes that confer resistance to gentamicin, streptomycin and spectinomycin, kanamycin and neomycin, ampicillin, chloramphenicol, sulfamethoxazole, and tetracycline, respectively. The backbone of this region is derived from Tn1721 and is interrupted by a hybrid Tn2670 (Tn21)-Tn1696-type transposon, Tn6020, and an incomplete Tn1. After minor rearrangements, this R1215 resistance island can generate AbGRI2-0, the predicted earliest form of the IS26-bounded AbGRI2-type resistance island of GC2 isolates, and to the multiple antibiotic resistance region (MARR) of AbaR0, the precursor of this region in AbaR-type resistance islands in the GC1 group. A 29.9-kb circle excised by IS26 has been inserted into the A. baumannii chromosome to generate AbGRI2-0. To create the MARR of AbaR0, a different circular form, again generated by IS26 from an R1215 resistance region variant, has been opened at a different point by recombination with a copy of the sul1 gene already present in the AbaR precursor. Recent IncM plasmids related to R1215 have a variant resistance island containing a blaSHV gene in the same location. IMPORTANCE Two lineages of extensively antibiotic-resistant A. baumannii currently plaguing modern medicine each acquired resistance to all of the original antibiotics (ampicillin, tetracycline, kanamycin, and sulfonamides) by the end of the 1970s and then became resistant to antibiotics from newer families after they were introduced in the 1980s. Here, we show that, in both of the dominant globally disseminated A. baumannii clones, a related set of antibiotic resistance genes was acquired together from the same resistance region that had already evolved in an IncM plasmid. In both cases, the action of IS26 was important in this process, but homologous recombination was also involved. The findings highlight the fact that complex regions carrying several resistance genes can evolve in one location or organism and all or part of the evolved region can then move to other locations and other organisms, conferring resistance to several antibiotics in a single step.
format	article
author	Grace A. Blackwell Mohammad Hamidian Ruth M. Hall
author_facet	Grace A. Blackwell Mohammad Hamidian Ruth M. Hall
author_sort	Grace A. Blackwell
title	IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated <named-content content-type="genus-species">Acinetobacter baumannii</named-content> GC1 and GC2 Clones
title_short	IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated <named-content content-type="genus-species">Acinetobacter baumannii</named-content> GC1 and GC2 Clones
title_full	IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated <named-content content-type="genus-species">Acinetobacter baumannii</named-content> GC1 and GC2 Clones
title_fullStr	IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated <named-content content-type="genus-species">Acinetobacter baumannii</named-content> GC1 and GC2 Clones
title_full_unstemmed	IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated <named-content content-type="genus-species">Acinetobacter baumannii</named-content> GC1 and GC2 Clones
title_sort	incm plasmid r1215 is the source of chromosomally located regions containing multiple antibiotic resistance genes in the globally disseminated <named-content content-type="genus-species">acinetobacter baumannii</named-content> gc1 and gc2 clones
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/44d9527e399146f2a138ed01c1d5349e
work_keys_str_mv	AT graceablackwell incmplasmidr1215isthesourceofchromosomallylocatedregionscontainingmultipleantibioticresistancegenesinthegloballydisseminatednamedcontentcontenttypegenusspeciesacinetobacterbaumanniinamedcontentgc1andgc2clones AT mohammadhamidian incmplasmidr1215isthesourceofchromosomallylocatedregionscontainingmultipleantibioticresistancegenesinthegloballydisseminatednamedcontentcontenttypegenusspeciesacinetobacterbaumanniinamedcontentgc1andgc2clones AT ruthmhall incmplasmidr1215isthesourceofchromosomallylocatedregionscontainingmultipleantibioticresistancegenesinthegloballydisseminatednamedcontentcontenttypegenusspeciesacinetobacterbaumanniinamedcontentgc1andgc2clones
_version_	1718428124559966208

IncM Plasmid R1215 Is the Source of Chromosomally Located Regions Containing Multiple Antibiotic Resistance Genes in the Globally Disseminated <named-content content-type="genus-species">Acinetobacter baumannii</named-content> GC1 and GC2 Clones

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