Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate

Abstract The oriental armyworm Mythimna separate is an economically important insect with a wide distribution and strong migratory activity. However, knowledge about the molecular mechanisms regulating the physiological and behavioural responses of the oriental armyworm is scarce. In the present stu...

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Autores principales:	Hai-Xu Bian, Hong-Fang Ma, Xi-Xi Zheng, Ming-Hui Peng, Yu-Ping Li, Jun-Fang Su, Huan Wang, Qun Li, Run-Xi Xia, Yan-Qun Liu, Xing-Fu Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/44d9b638a8884424bd095867b4369fc2
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spelling	oai:doaj.org-article:44d9b638a8884424bd095867b4369fc22021-12-02T15:05:56ZCharacterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate10.1038/s41598-017-02513-62045-2322https://doaj.org/article/44d9b638a8884424bd095867b4369fc22017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02513-6https://doaj.org/toc/2045-2322Abstract The oriental armyworm Mythimna separate is an economically important insect with a wide distribution and strong migratory activity. However, knowledge about the molecular mechanisms regulating the physiological and behavioural responses of the oriental armyworm is scarce. In the present study, we took a transcriptomic approach to characterize the gene network in the adult head of M. separate. The sequencing and de novo assembly yielded 63,499 transcripts, which were further assembled into 46,459 unigenes with an N50 of 1,153 bp. In the head transcriptome data, unigenes involved in the ‘signal transduction mechanism’ are the most abundant. In total, 937 signal transduction unigenes were assigned to 22 signalling pathways. The circadian clock, melanin synthesis, and non-receptor protein of olfactory gene families were then identified, and phylogenetic analyses were performed with these M. separate genes, the model insect Bombyx mori and other insects. Furthermore, 1,372 simple sequence repeats of 2–6 bp in unit length were identified. The transcriptome data represent a comprehensive molecular resource for the adult head of M. separate, and these identified genes can be valid targets for further gene function research to address the molecular mechanisms regulating the migratory and olfaction genes of the oriental armyworm.Hai-Xu BianHong-Fang MaXi-Xi ZhengMing-Hui PengYu-Ping LiJun-Fang SuHuan WangQun LiRun-Xi XiaYan-Qun LiuXing-Fu JiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hai-Xu Bian Hong-Fang Ma Xi-Xi Zheng Ming-Hui Peng Yu-Ping Li Jun-Fang Su Huan Wang Qun Li Run-Xi Xia Yan-Qun Liu Xing-Fu Jiang Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate
description	Abstract The oriental armyworm Mythimna separate is an economically important insect with a wide distribution and strong migratory activity. However, knowledge about the molecular mechanisms regulating the physiological and behavioural responses of the oriental armyworm is scarce. In the present study, we took a transcriptomic approach to characterize the gene network in the adult head of M. separate. The sequencing and de novo assembly yielded 63,499 transcripts, which were further assembled into 46,459 unigenes with an N50 of 1,153 bp. In the head transcriptome data, unigenes involved in the ‘signal transduction mechanism’ are the most abundant. In total, 937 signal transduction unigenes were assigned to 22 signalling pathways. The circadian clock, melanin synthesis, and non-receptor protein of olfactory gene families were then identified, and phylogenetic analyses were performed with these M. separate genes, the model insect Bombyx mori and other insects. Furthermore, 1,372 simple sequence repeats of 2–6 bp in unit length were identified. The transcriptome data represent a comprehensive molecular resource for the adult head of M. separate, and these identified genes can be valid targets for further gene function research to address the molecular mechanisms regulating the migratory and olfaction genes of the oriental armyworm.
format	article
author	Hai-Xu Bian Hong-Fang Ma Xi-Xi Zheng Ming-Hui Peng Yu-Ping Li Jun-Fang Su Huan Wang Qun Li Run-Xi Xia Yan-Qun Liu Xing-Fu Jiang
author_facet	Hai-Xu Bian Hong-Fang Ma Xi-Xi Zheng Ming-Hui Peng Yu-Ping Li Jun-Fang Su Huan Wang Qun Li Run-Xi Xia Yan-Qun Liu Xing-Fu Jiang
author_sort	Hai-Xu Bian
title	Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate
title_short	Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate
title_full	Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate
title_fullStr	Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate
title_full_unstemmed	Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate
title_sort	characterization of the adult head transcriptome and identification of migration and olfaction genes in the oriental armyworm mythimna separate
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/44d9b638a8884424bd095867b4369fc2
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Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate

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