Comparative Microbiota Composition Across Developmental Stages of Natural and Laboratory-Reared Chironomus circumdatus Populations From India

Chironomids are aquatic insects that undergo a complete metamorphosis of four life stages. Here we studied, for the first time, the microbiota composition of Chironomus circumdatus, a tropical midge species, both from the Mula and Mutha Rivers in Pune, India and as a laboratory-reared culture. We ge...

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Autores principales:	Sivan Laviad-Shitrit, Rotem Sela, Yehonatan Sharaby, Leena Thorat, Bimalendu B. Nath, Malka Halpern
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	microbiota bacterial composition chironomids Chironomus circumdatus developmental stages Cetobacterium Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/44da907cb86c438bba2d2d76bfdb0e39
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spelling	oai:doaj.org-article:44da907cb86c438bba2d2d76bfdb0e392021-12-01T06:09:09ZComparative Microbiota Composition Across Developmental Stages of Natural and Laboratory-Reared Chironomus circumdatus Populations From India1664-302X10.3389/fmicb.2021.746830https://doaj.org/article/44da907cb86c438bba2d2d76bfdb0e392021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.746830/fullhttps://doaj.org/toc/1664-302XChironomids are aquatic insects that undergo a complete metamorphosis of four life stages. Here we studied, for the first time, the microbiota composition of Chironomus circumdatus, a tropical midge species, both from the Mula and Mutha Rivers in Pune, India and as a laboratory-reared culture. We generated a comparative microbial profile of the eggs, larvae and pupae, the three aquatic life stages of C. circumdatus. Non-metric multidimensional scaling analysis (NMDS) demonstrated that the developmental stage had a more prominent effect on the microbiota composition compared to the sampling location. Notably, the microbiota composition of the egg masses from the different sampling points clustered together and differed from laboratory culture larvae. Proteobacteria was the dominant phylum in all the environmental and laboratory-reared egg masses and pupal samples, and in the laboratory-reared larvae, while Fusobacteria was the dominant phylum in the larvae collected from the field environment. The most abundant genera were Cetobacterium, Aeromonas, Dysgonomonas, Vibrio, and Flavobacterium. The ten amplicon sequence variants (ASVs) that most significantly contributed to differences in microbiota composition between the three sampled locations were: Burkholderiaceae (ASVs 04 and 37), C39 (Rhodocyclaceae, ASV 14), Vibrio (ASV 07), Arcobacter (ASV 21), Sphaerotilus (ASV 22), Bacteroidia (ASVs 12 and 28), Flavobacterium (ASV 29), and Gottschalkia (ASV 10). No significant differences were found in the microbial richness (Chao1) or diversity (Shannon H’) of the three sampled locations. In contrast, significant differences were found between the microbial richness of the three life stages. Studying the microbiota of this Chironomus species may contribute to a better understanding of the association of C. circumdatus and its microbial inhabitants.Sivan Laviad-ShitritRotem SelaYehonatan SharabyLeena ThoratLeena ThoratBimalendu B. NathMalka HalpernMalka HalpernFrontiers Media S.A.articlemicrobiotabacterial compositionchironomidsChironomus circumdatusdevelopmental stagesCetobacteriumMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microbiota bacterial composition chironomids Chironomus circumdatus developmental stages Cetobacterium Microbiology QR1-502
spellingShingle	microbiota bacterial composition chironomids Chironomus circumdatus developmental stages Cetobacterium Microbiology QR1-502 Sivan Laviad-Shitrit Rotem Sela Yehonatan Sharaby Leena Thorat Leena Thorat Bimalendu B. Nath Malka Halpern Malka Halpern Comparative Microbiota Composition Across Developmental Stages of Natural and Laboratory-Reared Chironomus circumdatus Populations From India
description	Chironomids are aquatic insects that undergo a complete metamorphosis of four life stages. Here we studied, for the first time, the microbiota composition of Chironomus circumdatus, a tropical midge species, both from the Mula and Mutha Rivers in Pune, India and as a laboratory-reared culture. We generated a comparative microbial profile of the eggs, larvae and pupae, the three aquatic life stages of C. circumdatus. Non-metric multidimensional scaling analysis (NMDS) demonstrated that the developmental stage had a more prominent effect on the microbiota composition compared to the sampling location. Notably, the microbiota composition of the egg masses from the different sampling points clustered together and differed from laboratory culture larvae. Proteobacteria was the dominant phylum in all the environmental and laboratory-reared egg masses and pupal samples, and in the laboratory-reared larvae, while Fusobacteria was the dominant phylum in the larvae collected from the field environment. The most abundant genera were Cetobacterium, Aeromonas, Dysgonomonas, Vibrio, and Flavobacterium. The ten amplicon sequence variants (ASVs) that most significantly contributed to differences in microbiota composition between the three sampled locations were: Burkholderiaceae (ASVs 04 and 37), C39 (Rhodocyclaceae, ASV 14), Vibrio (ASV 07), Arcobacter (ASV 21), Sphaerotilus (ASV 22), Bacteroidia (ASVs 12 and 28), Flavobacterium (ASV 29), and Gottschalkia (ASV 10). No significant differences were found in the microbial richness (Chao1) or diversity (Shannon H’) of the three sampled locations. In contrast, significant differences were found between the microbial richness of the three life stages. Studying the microbiota of this Chironomus species may contribute to a better understanding of the association of C. circumdatus and its microbial inhabitants.
format	article
author	Sivan Laviad-Shitrit Rotem Sela Yehonatan Sharaby Leena Thorat Leena Thorat Bimalendu B. Nath Malka Halpern Malka Halpern
author_facet	Sivan Laviad-Shitrit Rotem Sela Yehonatan Sharaby Leena Thorat Leena Thorat Bimalendu B. Nath Malka Halpern Malka Halpern
author_sort	Sivan Laviad-Shitrit
title	Comparative Microbiota Composition Across Developmental Stages of Natural and Laboratory-Reared Chironomus circumdatus Populations From India
title_short	Comparative Microbiota Composition Across Developmental Stages of Natural and Laboratory-Reared Chironomus circumdatus Populations From India
title_full	Comparative Microbiota Composition Across Developmental Stages of Natural and Laboratory-Reared Chironomus circumdatus Populations From India
title_fullStr	Comparative Microbiota Composition Across Developmental Stages of Natural and Laboratory-Reared Chironomus circumdatus Populations From India
title_full_unstemmed	Comparative Microbiota Composition Across Developmental Stages of Natural and Laboratory-Reared Chironomus circumdatus Populations From India
title_sort	comparative microbiota composition across developmental stages of natural and laboratory-reared chironomus circumdatus populations from india
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/44da907cb86c438bba2d2d76bfdb0e39
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Comparative Microbiota Composition Across Developmental Stages of Natural and Laboratory-Reared Chironomus circumdatus Populations From India

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