The study of correlations between B-Lactamase and protein A in Staphylococcus aureus

Objective: Staphylococcus aureus is the type species and pathogen of human. There is some factors that determines the virulence of bacteria, but protein A and B-Lactamase are the most important factors. We studied the correlations between two factors of virulence (B-Lactamase and protein A) in this...

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Autores principales:	R Kasra Kermanshahi, H Keyvani Amineh, M Gol Alipour
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2000
Materias:	staphylococcus aureus b-lactamase protein a antibiotic virulance Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/44dacc730a45472281aeac402af1567a
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spelling	oai:doaj.org-article:44dacc730a45472281aeac402af1567a2021-11-10T09:22:07ZThe study of correlations between B-Lactamase and protein A in Staphylococcus aureus1561-41072251-7170https://doaj.org/article/44dacc730a45472281aeac402af1567a2000-04-01T00:00:00Zhttp://jbums.org/article-1-2907-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170Objective: Staphylococcus aureus is the type species and pathogen of human. There is some factors that determines the virulence of bacteria, but protein A and B-Lactamase are the most important factors. We studied the correlations between two factors of virulence (B-Lactamase and protein A) in this microorganism. Methods: We studied the resistance to B-Lactamase sensible to B-Lactamase as penicillin G (Pen G), Cefazolin (CeZ), Cefalotine (Cfn), resistant to B-Lactamase as methicillin (Met) and Oxacilin (Oxa) with disk diffusion method. MICS and B-Lactamase production detected by broth dilution and acidometric methods. Purification of SPA performed on these samples with ion exchange chromatography method (DEAE-Cellulose). Cell wall lysis was performed with heat extraction method. Findings: These results showed that resistance to drugs was 96% (Pen G), 83% (CeZ), 86% (Cfn) and 73% (Oxa). Purification performed on these samples with method (DEAE-Cellulose) have a sharp dense band in electrophoresis (SDS-PAGE) with a molecular weight of about 42 kd. Conclusion: After studying relationship between existence of SPA and B-Lactamase, we determined that 93.7% of strains with B-Lactamase had SPA but the strains without B-Lactamase only 50% had SPA. The result with methicillin resistant strains is most representative because in these kinds of strains there are not any strains with SPA.R Kasra KermanshahiH Keyvani AminehM Gol AlipourBabol University of Medical Sciencesarticlestaphylococcus aureusb-lactamaseprotein aantibioticvirulanceMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 2, Iss 2, Pp 16-22 (2000)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	staphylococcus aureus b-lactamase protein a antibiotic virulance Medicine R Medicine (General) R5-920
spellingShingle	staphylococcus aureus b-lactamase protein a antibiotic virulance Medicine R Medicine (General) R5-920 R Kasra Kermanshahi H Keyvani Amineh M Gol Alipour The study of correlations between B-Lactamase and protein A in Staphylococcus aureus
description	Objective: Staphylococcus aureus is the type species and pathogen of human. There is some factors that determines the virulence of bacteria, but protein A and B-Lactamase are the most important factors. We studied the correlations between two factors of virulence (B-Lactamase and protein A) in this microorganism. Methods: We studied the resistance to B-Lactamase sensible to B-Lactamase as penicillin G (Pen G), Cefazolin (CeZ), Cefalotine (Cfn), resistant to B-Lactamase as methicillin (Met) and Oxacilin (Oxa) with disk diffusion method. MICS and B-Lactamase production detected by broth dilution and acidometric methods. Purification of SPA performed on these samples with ion exchange chromatography method (DEAE-Cellulose). Cell wall lysis was performed with heat extraction method. Findings: These results showed that resistance to drugs was 96% (Pen G), 83% (CeZ), 86% (Cfn) and 73% (Oxa). Purification performed on these samples with method (DEAE-Cellulose) have a sharp dense band in electrophoresis (SDS-PAGE) with a molecular weight of about 42 kd. Conclusion: After studying relationship between existence of SPA and B-Lactamase, we determined that 93.7% of strains with B-Lactamase had SPA but the strains without B-Lactamase only 50% had SPA. The result with methicillin resistant strains is most representative because in these kinds of strains there are not any strains with SPA.
format	article
author	R Kasra Kermanshahi H Keyvani Amineh M Gol Alipour
author_facet	R Kasra Kermanshahi H Keyvani Amineh M Gol Alipour
author_sort	R Kasra Kermanshahi
title	The study of correlations between B-Lactamase and protein A in Staphylococcus aureus
title_short	The study of correlations between B-Lactamase and protein A in Staphylococcus aureus
title_full	The study of correlations between B-Lactamase and protein A in Staphylococcus aureus
title_fullStr	The study of correlations between B-Lactamase and protein A in Staphylococcus aureus
title_full_unstemmed	The study of correlations between B-Lactamase and protein A in Staphylococcus aureus
title_sort	study of correlations between b-lactamase and protein a in staphylococcus aureus
publisher	Babol University of Medical Sciences
publishDate	2000
url	https://doaj.org/article/44dacc730a45472281aeac402af1567a
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The study of correlations between B-Lactamase and protein A in Staphylococcus aureus

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