Elevated expression of NFE2L3 promotes the development of gastric cancer through epithelial-mesenchymal transformation

Gastric cancer (GC) is a malignant tumour with high mortality, but research on its molecular mechanisms remain limited. This study is the first to explore the biological role of nuclear factor NFE2L3 (nuclear factor, erythroid 2 like 3) in GC. We used Western blot and RT–qPCR to detect gene expressi...

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Autores principales:	Xiaodong Wang, Yaxian Li, Ziqing Fang, Yongxiang Li
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	gastric cancer emt nfe2l3 bioinformatics Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/44e35ed87b3146d899941f66f478d04a
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spelling	oai:doaj.org-article:44e35ed87b3146d899941f66f478d04a2021-11-17T14:21:59ZElevated expression of NFE2L3 promotes the development of gastric cancer through epithelial-mesenchymal transformation2165-59792165-598710.1080/21655979.2021.2005915https://doaj.org/article/44e35ed87b3146d899941f66f478d04a2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2005915https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Gastric cancer (GC) is a malignant tumour with high mortality, but research on its molecular mechanisms remain limited. This study is the first to explore the biological role of nuclear factor NFE2L3 (nuclear factor, erythroid 2 like 3) in GC. We used Western blot and RT–qPCR to detect gene expression at the protein or mRNA level. Short hairpin RNA (shRNA) transfection was used to inhibit NFE2L3 expression. CCK-8 and colony formation assays were used to detect cell proliferation. Cell migration, invasion, cell cycle and apoptosis were detected by Transwell assays and flow cytometry. The results showed that NFE2L3 was highly expressed in gastric cancer tissues and promoted gastric cancer cell proliferation and metastasis. Inhibiting NFE2L3 expression blocks the cell cycle and increases the proportion of apoptotic cells, whereas NFE2L3 expression promotes the epithelial-mesenchymal transformation (EMT) process. In summary, NFE2L3 is highly expressed in gastric cancer and promotes gastric cancer cell proliferation and metastasis and the EMT process.Xiaodong WangYaxian LiZiqing FangYongxiang LiTaylor & Francis Grouparticlegastric canceremtnfe2l3bioinformaticsBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gastric cancer emt nfe2l3 bioinformatics Biotechnology TP248.13-248.65
spellingShingle	gastric cancer emt nfe2l3 bioinformatics Biotechnology TP248.13-248.65 Xiaodong Wang Yaxian Li Ziqing Fang Yongxiang Li Elevated expression of NFE2L3 promotes the development of gastric cancer through epithelial-mesenchymal transformation
description	Gastric cancer (GC) is a malignant tumour with high mortality, but research on its molecular mechanisms remain limited. This study is the first to explore the biological role of nuclear factor NFE2L3 (nuclear factor, erythroid 2 like 3) in GC. We used Western blot and RT–qPCR to detect gene expression at the protein or mRNA level. Short hairpin RNA (shRNA) transfection was used to inhibit NFE2L3 expression. CCK-8 and colony formation assays were used to detect cell proliferation. Cell migration, invasion, cell cycle and apoptosis were detected by Transwell assays and flow cytometry. The results showed that NFE2L3 was highly expressed in gastric cancer tissues and promoted gastric cancer cell proliferation and metastasis. Inhibiting NFE2L3 expression blocks the cell cycle and increases the proportion of apoptotic cells, whereas NFE2L3 expression promotes the epithelial-mesenchymal transformation (EMT) process. In summary, NFE2L3 is highly expressed in gastric cancer and promotes gastric cancer cell proliferation and metastasis and the EMT process.
format	article
author	Xiaodong Wang Yaxian Li Ziqing Fang Yongxiang Li
author_facet	Xiaodong Wang Yaxian Li Ziqing Fang Yongxiang Li
author_sort	Xiaodong Wang
title	Elevated expression of NFE2L3 promotes the development of gastric cancer through epithelial-mesenchymal transformation
title_short	Elevated expression of NFE2L3 promotes the development of gastric cancer through epithelial-mesenchymal transformation
title_full	Elevated expression of NFE2L3 promotes the development of gastric cancer through epithelial-mesenchymal transformation
title_fullStr	Elevated expression of NFE2L3 promotes the development of gastric cancer through epithelial-mesenchymal transformation
title_full_unstemmed	Elevated expression of NFE2L3 promotes the development of gastric cancer through epithelial-mesenchymal transformation
title_sort	elevated expression of nfe2l3 promotes the development of gastric cancer through epithelial-mesenchymal transformation
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/44e35ed87b3146d899941f66f478d04a
work_keys_str_mv	AT xiaodongwang elevatedexpressionofnfe2l3promotesthedevelopmentofgastriccancerthroughepithelialmesenchymaltransformation AT yaxianli elevatedexpressionofnfe2l3promotesthedevelopmentofgastriccancerthroughepithelialmesenchymaltransformation AT ziqingfang elevatedexpressionofnfe2l3promotesthedevelopmentofgastriccancerthroughepithelialmesenchymaltransformation AT yongxiangli elevatedexpressionofnfe2l3promotesthedevelopmentofgastriccancerthroughepithelialmesenchymaltransformation
_version_	1718425411501686784

Elevated expression of NFE2L3 promotes the development of gastric cancer through epithelial-mesenchymal transformation

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