Ex vivo immune profiling in patient blood enables quantification of innate immune effector functions

Abstract The assessment of a patient’s immune function is critical in many clinical situations. In complex clinical immune dysfunction like sepsis, which results from a loss of immune homeostasis due to microbial infection, a plethora of pro- and anti-inflammatory stimuli may occur consecutively or...

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Autores principales:	Teresa Lehnert, Ines Leonhardt, Sandra Timme, Daniel Thomas-Rüddel, Frank Bloos, Christoph Sponholz, Oliver Kurzai, Marc Thilo Figge, Kerstin Hünniger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/44e5ddd83d8842ec9aabf80b07ef38dc
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spelling	oai:doaj.org-article:44e5ddd83d8842ec9aabf80b07ef38dc2021-12-02T17:38:26ZEx vivo immune profiling in patient blood enables quantification of innate immune effector functions10.1038/s41598-021-91362-52045-2322https://doaj.org/article/44e5ddd83d8842ec9aabf80b07ef38dc2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91362-5https://doaj.org/toc/2045-2322Abstract The assessment of a patient’s immune function is critical in many clinical situations. In complex clinical immune dysfunction like sepsis, which results from a loss of immune homeostasis due to microbial infection, a plethora of pro- and anti-inflammatory stimuli may occur consecutively or simultaneously. Thus, any immunomodulatory therapy would require in-depth knowledge of an individual patient’s immune status at a given time. Whereas lab-based immune profiling often relies solely on quantification of cell numbers, we used an ex vivo whole-blood infection model in combination with biomathematical modeling to quantify functional parameters of innate immune cells in blood from patients undergoing cardiac surgery. These patients experience a well-characterized inflammatory insult, which results in mitigation of the pathogen-specific response patterns towards Staphylococcus aureus and Candida albicans that are characteristic of healthy people and our patients at baseline. This not only interferes with the elimination of these pathogens from blood, but also selectively augments the escape of C. albicans from phagocytosis. In summary, our model could serve as a valuable functional immune assay for recording and evaluating innate responses to infection.Teresa LehnertInes LeonhardtSandra TimmeDaniel Thomas-RüddelFrank BloosChristoph SponholzOliver KurzaiMarc Thilo FiggeKerstin HünnigerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Teresa Lehnert Ines Leonhardt Sandra Timme Daniel Thomas-Rüddel Frank Bloos Christoph Sponholz Oliver Kurzai Marc Thilo Figge Kerstin Hünniger Ex vivo immune profiling in patient blood enables quantification of innate immune effector functions
description	Abstract The assessment of a patient’s immune function is critical in many clinical situations. In complex clinical immune dysfunction like sepsis, which results from a loss of immune homeostasis due to microbial infection, a plethora of pro- and anti-inflammatory stimuli may occur consecutively or simultaneously. Thus, any immunomodulatory therapy would require in-depth knowledge of an individual patient’s immune status at a given time. Whereas lab-based immune profiling often relies solely on quantification of cell numbers, we used an ex vivo whole-blood infection model in combination with biomathematical modeling to quantify functional parameters of innate immune cells in blood from patients undergoing cardiac surgery. These patients experience a well-characterized inflammatory insult, which results in mitigation of the pathogen-specific response patterns towards Staphylococcus aureus and Candida albicans that are characteristic of healthy people and our patients at baseline. This not only interferes with the elimination of these pathogens from blood, but also selectively augments the escape of C. albicans from phagocytosis. In summary, our model could serve as a valuable functional immune assay for recording and evaluating innate responses to infection.
format	article
author	Teresa Lehnert Ines Leonhardt Sandra Timme Daniel Thomas-Rüddel Frank Bloos Christoph Sponholz Oliver Kurzai Marc Thilo Figge Kerstin Hünniger
author_facet	Teresa Lehnert Ines Leonhardt Sandra Timme Daniel Thomas-Rüddel Frank Bloos Christoph Sponholz Oliver Kurzai Marc Thilo Figge Kerstin Hünniger
author_sort	Teresa Lehnert
title	Ex vivo immune profiling in patient blood enables quantification of innate immune effector functions
title_short	Ex vivo immune profiling in patient blood enables quantification of innate immune effector functions
title_full	Ex vivo immune profiling in patient blood enables quantification of innate immune effector functions
title_fullStr	Ex vivo immune profiling in patient blood enables quantification of innate immune effector functions
title_full_unstemmed	Ex vivo immune profiling in patient blood enables quantification of innate immune effector functions
title_sort	ex vivo immune profiling in patient blood enables quantification of innate immune effector functions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/44e5ddd83d8842ec9aabf80b07ef38dc
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Ex vivo immune profiling in patient blood enables quantification of innate immune effector functions

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