Rapid coupling between gravitational forces and the transcriptome in human myelomonocytic U937 cells

Abstract The gravitational force has been constant throughout Earth’s evolutionary history. Since the cell nucleus is subjected to permanent forces induced by Earth’s gravity, we addressed the question, if gene expression homeostasis is constantly shaped by the gravitational force on Earth. We there...

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Autores principales:	Cora S. Thiel, Svantje Tauber, Swantje Christoffel, Andreas Huge, Beatrice A. Lauber, Jennifer Polzer, Katrin Paulsen, Hartwin Lier, Frank Engelmann, Burkhard Schmitz, Andreas Schütte, Christiane Raig, Liliana E. Layer, Oliver Ullrich
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/44e64d0c24c0444c8c368d6412db525b
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spelling	oai:doaj.org-article:44e64d0c24c0444c8c368d6412db525b2021-12-02T15:08:12ZRapid coupling between gravitational forces and the transcriptome in human myelomonocytic U937 cells10.1038/s41598-018-31596-y2045-2322https://doaj.org/article/44e64d0c24c0444c8c368d6412db525b2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-31596-yhttps://doaj.org/toc/2045-2322Abstract The gravitational force has been constant throughout Earth’s evolutionary history. Since the cell nucleus is subjected to permanent forces induced by Earth’s gravity, we addressed the question, if gene expression homeostasis is constantly shaped by the gravitational force on Earth. We therefore investigated the transcriptome in force-free conditions of microgravity, determined the time frame of initial gravitational force-transduction to the transcriptome and assessed the role of cation channels. We combined a parabolic flight experiment campaign with a suborbital ballistic rocket experiment employing the human myelomonocytic cell line U937 and analyzed the whole gene transcription by microarray, using rigorous controls for exclusion of effects not related to gravitational force and cross-validation through two fully independent research campaigns. Experiments with the wide range ion channel inhibitor SKF-96365 in combination with whole transcriptome analysis were conducted to study the functional role of ion channels in the transduction of gravitational forces at an integrative level. We detected profound alterations in the transcriptome already after 20 s of microgravity or hypergravity. In microgravity, 99.43% of all initially altered transcripts adapted after 5 min. In hypergravity, 98.93% of all initially altered transcripts adapted after 75 s. Only 2.4% of all microgravity-regulated transcripts were sensitive to the cation channel inhibitor SKF-96365. Inter-platform comparison of differentially regulated transcripts revealed 57 annotated gravity-sensitive transcripts. We assume that gravitational forces are rapidly and constantly transduced into the nucleus as omnipresent condition for nuclear and chromatin structure as well as homeostasis of gene expression.Cora S. ThielSvantje TauberSwantje ChristoffelAndreas HugeBeatrice A. LauberJennifer PolzerKatrin PaulsenHartwin LierFrank EngelmannBurkhard SchmitzAndreas SchütteChristiane RaigLiliana E. LayerOliver UllrichNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-24 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cora S. Thiel Svantje Tauber Swantje Christoffel Andreas Huge Beatrice A. Lauber Jennifer Polzer Katrin Paulsen Hartwin Lier Frank Engelmann Burkhard Schmitz Andreas Schütte Christiane Raig Liliana E. Layer Oliver Ullrich Rapid coupling between gravitational forces and the transcriptome in human myelomonocytic U937 cells
description	Abstract The gravitational force has been constant throughout Earth’s evolutionary history. Since the cell nucleus is subjected to permanent forces induced by Earth’s gravity, we addressed the question, if gene expression homeostasis is constantly shaped by the gravitational force on Earth. We therefore investigated the transcriptome in force-free conditions of microgravity, determined the time frame of initial gravitational force-transduction to the transcriptome and assessed the role of cation channels. We combined a parabolic flight experiment campaign with a suborbital ballistic rocket experiment employing the human myelomonocytic cell line U937 and analyzed the whole gene transcription by microarray, using rigorous controls for exclusion of effects not related to gravitational force and cross-validation through two fully independent research campaigns. Experiments with the wide range ion channel inhibitor SKF-96365 in combination with whole transcriptome analysis were conducted to study the functional role of ion channels in the transduction of gravitational forces at an integrative level. We detected profound alterations in the transcriptome already after 20 s of microgravity or hypergravity. In microgravity, 99.43% of all initially altered transcripts adapted after 5 min. In hypergravity, 98.93% of all initially altered transcripts adapted after 75 s. Only 2.4% of all microgravity-regulated transcripts were sensitive to the cation channel inhibitor SKF-96365. Inter-platform comparison of differentially regulated transcripts revealed 57 annotated gravity-sensitive transcripts. We assume that gravitational forces are rapidly and constantly transduced into the nucleus as omnipresent condition for nuclear and chromatin structure as well as homeostasis of gene expression.
format	article
author	Cora S. Thiel Svantje Tauber Swantje Christoffel Andreas Huge Beatrice A. Lauber Jennifer Polzer Katrin Paulsen Hartwin Lier Frank Engelmann Burkhard Schmitz Andreas Schütte Christiane Raig Liliana E. Layer Oliver Ullrich
author_facet	Cora S. Thiel Svantje Tauber Swantje Christoffel Andreas Huge Beatrice A. Lauber Jennifer Polzer Katrin Paulsen Hartwin Lier Frank Engelmann Burkhard Schmitz Andreas Schütte Christiane Raig Liliana E. Layer Oliver Ullrich
author_sort	Cora S. Thiel
title	Rapid coupling between gravitational forces and the transcriptome in human myelomonocytic U937 cells
title_short	Rapid coupling between gravitational forces and the transcriptome in human myelomonocytic U937 cells
title_full	Rapid coupling between gravitational forces and the transcriptome in human myelomonocytic U937 cells
title_fullStr	Rapid coupling between gravitational forces and the transcriptome in human myelomonocytic U937 cells
title_full_unstemmed	Rapid coupling between gravitational forces and the transcriptome in human myelomonocytic U937 cells
title_sort	rapid coupling between gravitational forces and the transcriptome in human myelomonocytic u937 cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/44e64d0c24c0444c8c368d6412db525b
work_keys_str_mv	AT corasthiel rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT svantjetauber rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT swantjechristoffel rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT andreashuge rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT beatricealauber rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT jenniferpolzer rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT katrinpaulsen rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT hartwinlier rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT frankengelmann rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT burkhardschmitz rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT andreasschutte rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT christianeraig rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT lilianaelayer rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells AT oliverullrich rapidcouplingbetweengravitationalforcesandthetranscriptomeinhumanmyelomonocyticu937cells
_version_	1718388248048304128

Rapid coupling between gravitational forces and the transcriptome in human myelomonocytic U937 cells

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