Choice of 16S ribosomal RNA primers affects the microbiome analysis in chicken ceca

Abstract We evaluated the effect of applying different sets of 16S rRNA primers on bacterial composition, diversity, and predicted function in chicken ceca. Cecal contents from Ross 708 birds at 1, 3, and 5 weeks of age were collected for DNA isolation. Eight different primer pairs targeting differe...

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Autores principales: Nadia Darwish, Jonathan Shao, Lori L. Schreier, Monika Proszkowiec-Weglarz
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/44fc7a844a5d40c9b188cebcab095753
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