Quorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium <i>Hafnia alvei</i>

Quorum sensing (QS) is widely present in microorganisms in marine aquatic products. Owing to the use of antibiotics, many spoilage bacteria in aquatic products are drug resistant. In order to slow down this evolutionary trend, the inhibition of spoilage phenotype of spoilage bacteria by interfering...

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Autores principales:	Yue Shen, Fangchao Cui, Dangfeng Wang, Tingting Li, Jianrong Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	quorum sensing quenching enzyme <i>Hafnia alvei</i> biofilm extracellular protease motility Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/44fd62b6b0694d709d4c2566b4dfcc40
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spelling	oai:doaj.org-article:44fd62b6b0694d709d4c2566b4dfcc402021-11-25T17:34:20ZQuorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium <i>Hafnia alvei</i>10.3390/foods101127002304-8158https://doaj.org/article/44fd62b6b0694d709d4c2566b4dfcc402021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2700https://doaj.org/toc/2304-8158Quorum sensing (QS) is widely present in microorganisms in marine aquatic products. Owing to the use of antibiotics, many spoilage bacteria in aquatic products are drug resistant. In order to slow down this evolutionary trend, the inhibition of spoilage phenotype of spoilage bacteria by interfering with QS has become a research hot spot in recent years. In this study, we found a new QS quenching enzyme, PF-1240; it was cloned and expressed in <i>Pseudomonas fluorescens</i> 08. Sequence alignment showed that its similarity with <i>N</i>-homoserine lactone (AHL) acylase QuiP protein of <i>Pseudomonas fluorescens</i> (<i>Pf</i> 0-1) was 78.4%. SDS-PAGE confirmed that the protein is a dimer composed of two subunits, which is similar to the structure of AHL acylases. The concentration of heterologous expression in <i>Escherichia coli</i> (DE3) was 26.64 μg/mL. Unlike most AHL acylases, PF-1240 can quench AHLs with different carbon chain lengths and inhibit the quorum sensing of the aquatic spoilage bacterium <i>Hafnia alvei</i>. It can significantly reduce the formation rate of biofilm of <i>H. alvei</i> to 44.4% and the yield of siderophores to 54%, inhibit the production of protease and lipase, and interfere with the motility of <i>H. alvei</i>. Through these corruption phenotypes, the specific application effect of PF-1240 can be further determined to provide a theoretical basis for its application in the preservation of practical aquatic products.Yue ShenFangchao CuiDangfeng WangTingting LiJianrong LiMDPI AGarticlequorum sensing quenching enzyme<i>Hafnia alvei</i>biofilmextracellular proteasemotilityChemical technologyTP1-1185ENFoods, Vol 10, Iss 2700, p 2700 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	quorum sensing quenching enzyme <i>Hafnia alvei</i> biofilm extracellular protease motility Chemical technology TP1-1185
spellingShingle	quorum sensing quenching enzyme <i>Hafnia alvei</i> biofilm extracellular protease motility Chemical technology TP1-1185 Yue Shen Fangchao Cui Dangfeng Wang Tingting Li Jianrong Li Quorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium <i>Hafnia alvei</i>
description	Quorum sensing (QS) is widely present in microorganisms in marine aquatic products. Owing to the use of antibiotics, many spoilage bacteria in aquatic products are drug resistant. In order to slow down this evolutionary trend, the inhibition of spoilage phenotype of spoilage bacteria by interfering with QS has become a research hot spot in recent years. In this study, we found a new QS quenching enzyme, PF-1240; it was cloned and expressed in <i>Pseudomonas fluorescens</i> 08. Sequence alignment showed that its similarity with <i>N</i>-homoserine lactone (AHL) acylase QuiP protein of <i>Pseudomonas fluorescens</i> (<i>Pf</i> 0-1) was 78.4%. SDS-PAGE confirmed that the protein is a dimer composed of two subunits, which is similar to the structure of AHL acylases. The concentration of heterologous expression in <i>Escherichia coli</i> (DE3) was 26.64 μg/mL. Unlike most AHL acylases, PF-1240 can quench AHLs with different carbon chain lengths and inhibit the quorum sensing of the aquatic spoilage bacterium <i>Hafnia alvei</i>. It can significantly reduce the formation rate of biofilm of <i>H. alvei</i> to 44.4% and the yield of siderophores to 54%, inhibit the production of protease and lipase, and interfere with the motility of <i>H. alvei</i>. Through these corruption phenotypes, the specific application effect of PF-1240 can be further determined to provide a theoretical basis for its application in the preservation of practical aquatic products.
format	article
author	Yue Shen Fangchao Cui Dangfeng Wang Tingting Li Jianrong Li
author_facet	Yue Shen Fangchao Cui Dangfeng Wang Tingting Li Jianrong Li
author_sort	Yue Shen
title	Quorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium <i>Hafnia alvei</i>
title_short	Quorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium <i>Hafnia alvei</i>
title_full	Quorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium <i>Hafnia alvei</i>
title_fullStr	Quorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium <i>Hafnia alvei</i>
title_full_unstemmed	Quorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium <i>Hafnia alvei</i>
title_sort	quorum quenching enzyme (pf-1240) capable to degrade ahls as a candidate for inhibiting quorum sensing in food spoilage bacterium <i>hafnia alvei</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/44fd62b6b0694d709d4c2566b4dfcc40
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Quorum Quenching Enzyme (PF-1240) Capable to Degrade AHLs as a Candidate for Inhibiting Quorum Sensing in Food Spoilage Bacterium <i>Hafnia alvei</i>

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