Genetic diversity of four broodstocks of tilapia (Oreochromis sp.) from Antioquia, Colombia

Background: Tilapia is the most farmed fish in Colombia. However, the genetic diversity and structure of broodstocks in the hatcheries of Antioquia province remains unknown. Objective: To analyze the genetic diversity and structure of one Nile and three red tilapia broodstocks in Antioquia, Colombia...

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Autores principales:	Andrés F. Montoya-López, Ariel M. Tarazona Morales, Martha Olivera-Angel, John J. Betancur-López
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2019
Materias:	aquaculture aquiculture assisted selection fish fisheries genetic diversity molecular markers stock management tilapia Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/4500d001a62d4a21925a0fefc413c5fb
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spelling	oai:doaj.org-article:4500d001a62d4a21925a0fefc413c5fb2021-12-01T18:49:31ZGenetic diversity of four broodstocks of tilapia (Oreochromis sp.) from Antioquia, Colombia2256-295810.17533/udea.rccp.v32n3a05https://doaj.org/article/4500d001a62d4a21925a0fefc413c5fb2019-07-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/337095https://doaj.org/toc/2256-2958Background: Tilapia is the most farmed fish in Colombia. However, the genetic diversity and structure of broodstocks in the hatcheries of Antioquia province remains unknown. Objective: To analyze the genetic diversity and structure of one Nile and three red tilapia broodstocks in Antioquia, Colombia. Methods: Fish were genotyped using 24 microsatellite markers of 13 linkage groups in five multiple reactions. Genetic diversity metrics were estimated and null alleles were detected. Analysis of Molecular Variance and analysis of number of clusters were used to describe the relationship between broodstocks. Results: Two microsatellites could not be amplified, and 22 were polymorphic. Average number of alleles per locus ranged 5.77 to 7.91. Locus UNH211 had the most alleles (17), whereas OMO032 had the fewest (4). Except for GM234 and OMO032, the analyzed loci had at least one private allele per population. Average effective number of alleles (3.37–4.03) was always less than the number of observed alleles. Significant deviations from Hardy-Weinberg equilibrium with heterozygote deficiencies were registered. Nine markers showed evidence of null alleles. The expected heterozygosity (0.65 to 0.67 per broodstock) was significantly higher than the observed heterozygosity (0.601 to 0.649) in the four populations. The fixation index for all broodstocks (excluding null alleles) was 0.0766 (95% confidence interval, 0.05092 to 0.10289). According to the molecular variance analysis, the greatest variation was between individuals rather than between groups of broodstocks or individuals within broodstocks. The genetic distance between the Nile and red broodstocks ranged from 0.43 to 0.54. Conclusions: Overall, these findings provide baseline information about the genetic diversity and structure of tilapia broodstocks in Antioquia, Colombia, useful for the management of hatcheries.Andrés F. Montoya-LópezAriel M. Tarazona MoralesMartha Olivera-AngelJohn J. Betancur-LópezUniversidad de Antioquiaarticleaquacultureaquicultureassisted selectionfishfisheriesgenetic diversitymolecular markersstock managementtilapiaAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 32, Iss 3, Pp 201-213 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	aquaculture aquiculture assisted selection fish fisheries genetic diversity molecular markers stock management tilapia Animal culture SF1-1100
spellingShingle	aquaculture aquiculture assisted selection fish fisheries genetic diversity molecular markers stock management tilapia Animal culture SF1-1100 Andrés F. Montoya-López Ariel M. Tarazona Morales Martha Olivera-Angel John J. Betancur-López Genetic diversity of four broodstocks of tilapia (Oreochromis sp.) from Antioquia, Colombia
description	Background: Tilapia is the most farmed fish in Colombia. However, the genetic diversity and structure of broodstocks in the hatcheries of Antioquia province remains unknown. Objective: To analyze the genetic diversity and structure of one Nile and three red tilapia broodstocks in Antioquia, Colombia. Methods: Fish were genotyped using 24 microsatellite markers of 13 linkage groups in five multiple reactions. Genetic diversity metrics were estimated and null alleles were detected. Analysis of Molecular Variance and analysis of number of clusters were used to describe the relationship between broodstocks. Results: Two microsatellites could not be amplified, and 22 were polymorphic. Average number of alleles per locus ranged 5.77 to 7.91. Locus UNH211 had the most alleles (17), whereas OMO032 had the fewest (4). Except for GM234 and OMO032, the analyzed loci had at least one private allele per population. Average effective number of alleles (3.37–4.03) was always less than the number of observed alleles. Significant deviations from Hardy-Weinberg equilibrium with heterozygote deficiencies were registered. Nine markers showed evidence of null alleles. The expected heterozygosity (0.65 to 0.67 per broodstock) was significantly higher than the observed heterozygosity (0.601 to 0.649) in the four populations. The fixation index for all broodstocks (excluding null alleles) was 0.0766 (95% confidence interval, 0.05092 to 0.10289). According to the molecular variance analysis, the greatest variation was between individuals rather than between groups of broodstocks or individuals within broodstocks. The genetic distance between the Nile and red broodstocks ranged from 0.43 to 0.54. Conclusions: Overall, these findings provide baseline information about the genetic diversity and structure of tilapia broodstocks in Antioquia, Colombia, useful for the management of hatcheries.
format	article
author	Andrés F. Montoya-López Ariel M. Tarazona Morales Martha Olivera-Angel John J. Betancur-López
author_facet	Andrés F. Montoya-López Ariel M. Tarazona Morales Martha Olivera-Angel John J. Betancur-López
author_sort	Andrés F. Montoya-López
title	Genetic diversity of four broodstocks of tilapia (Oreochromis sp.) from Antioquia, Colombia
title_short	Genetic diversity of four broodstocks of tilapia (Oreochromis sp.) from Antioquia, Colombia
title_full	Genetic diversity of four broodstocks of tilapia (Oreochromis sp.) from Antioquia, Colombia
title_fullStr	Genetic diversity of four broodstocks of tilapia (Oreochromis sp.) from Antioquia, Colombia
title_full_unstemmed	Genetic diversity of four broodstocks of tilapia (Oreochromis sp.) from Antioquia, Colombia
title_sort	genetic diversity of four broodstocks of tilapia (oreochromis sp.) from antioquia, colombia
publisher	Universidad de Antioquia
publishDate	2019
url	https://doaj.org/article/4500d001a62d4a21925a0fefc413c5fb
work_keys_str_mv	AT andresfmontoyalopez geneticdiversityoffourbroodstocksoftilapiaoreochromisspfromantioquiacolombia AT arielmtarazonamorales geneticdiversityoffourbroodstocksoftilapiaoreochromisspfromantioquiacolombia AT marthaoliveraangel geneticdiversityoffourbroodstocksoftilapiaoreochromisspfromantioquiacolombia AT johnjbetancurlopez geneticdiversityoffourbroodstocksoftilapiaoreochromisspfromantioquiacolombia
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Genetic diversity of four broodstocks of tilapia (Oreochromis sp.) from Antioquia, Colombia

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