Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells

Abstract Successful cell therapy for Parkinson’s disease (PD) requires large numbers of homogeneous ventral mesencephalic dopaminergic (vmDA) precursors. Enrichment of vmDA precursors via cell sorting is required to ensure high safety and efficacy of the cell therapy. Here, using LMX1A-eGFP knock-in...

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Autores principales:	Jeong-Eun Yoo, Dongjin R. Lee, Sanghyun Park, Hye-Rim Shin, Kun Gu Lee, Dae-Sung Kim, Mi-Young Jo, Jang-Hyeon Eom, Myung Soo Cho, Dong-Youn Hwang, Dong-Wook Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/4503967311b2473da2382379507f5f03
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spelling	oai:doaj.org-article:4503967311b2473da2382379507f5f032021-12-02T16:50:26ZTrophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells10.1038/s41531-021-00204-82373-8057https://doaj.org/article/4503967311b2473da2382379507f5f032021-07-01T00:00:00Zhttps://doi.org/10.1038/s41531-021-00204-8https://doaj.org/toc/2373-8057Abstract Successful cell therapy for Parkinson’s disease (PD) requires large numbers of homogeneous ventral mesencephalic dopaminergic (vmDA) precursors. Enrichment of vmDA precursors via cell sorting is required to ensure high safety and efficacy of the cell therapy. Here, using LMX1A-eGFP knock-in reporter human embryonic stem cells, we discovered a novel surface antigen, trophoblast glycoprotein (TPBG), which was preferentially expressed in vmDA precursors. TPBG-targeted cell sorting enriched FOXA2+LMX1A+ vmDA precursors and helped attain efficient behavioral recovery of rodent PD models with increased numbers of TH+, NURR1+, and PITX3+ vmDA neurons in the grafts. Additionally, fewer proliferating cells were detected in TPBG+ cell-derived grafts than in TPBG− cell-derived grafts. Our approach is an efficient way to obtain enriched bona fide vmDA precursors, which could open a new avenue for effective PD treatment.Jeong-Eun YooDongjin R. LeeSanghyun ParkHye-Rim ShinKun Gu LeeDae-Sung KimMi-Young JoJang-Hyeon EomMyung Soo ChoDong-Youn HwangDong-Wook KimNature PortfolioarticleNeurology. Diseases of the nervous systemRC346-429ENnpj Parkinson's Disease, Vol 7, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neurology. Diseases of the nervous system RC346-429
spellingShingle	Neurology. Diseases of the nervous system RC346-429 Jeong-Eun Yoo Dongjin R. Lee Sanghyun Park Hye-Rim Shin Kun Gu Lee Dae-Sung Kim Mi-Young Jo Jang-Hyeon Eom Myung Soo Cho Dong-Youn Hwang Dong-Wook Kim Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells
description	Abstract Successful cell therapy for Parkinson’s disease (PD) requires large numbers of homogeneous ventral mesencephalic dopaminergic (vmDA) precursors. Enrichment of vmDA precursors via cell sorting is required to ensure high safety and efficacy of the cell therapy. Here, using LMX1A-eGFP knock-in reporter human embryonic stem cells, we discovered a novel surface antigen, trophoblast glycoprotein (TPBG), which was preferentially expressed in vmDA precursors. TPBG-targeted cell sorting enriched FOXA2+LMX1A+ vmDA precursors and helped attain efficient behavioral recovery of rodent PD models with increased numbers of TH+, NURR1+, and PITX3+ vmDA neurons in the grafts. Additionally, fewer proliferating cells were detected in TPBG+ cell-derived grafts than in TPBG− cell-derived grafts. Our approach is an efficient way to obtain enriched bona fide vmDA precursors, which could open a new avenue for effective PD treatment.
format	article
author	Jeong-Eun Yoo Dongjin R. Lee Sanghyun Park Hye-Rim Shin Kun Gu Lee Dae-Sung Kim Mi-Young Jo Jang-Hyeon Eom Myung Soo Cho Dong-Youn Hwang Dong-Wook Kim
author_facet	Jeong-Eun Yoo Dongjin R. Lee Sanghyun Park Hye-Rim Shin Kun Gu Lee Dae-Sung Kim Mi-Young Jo Jang-Hyeon Eom Myung Soo Cho Dong-Youn Hwang Dong-Wook Kim
author_sort	Jeong-Eun Yoo
title	Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells
title_short	Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells
title_full	Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells
title_fullStr	Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells
title_full_unstemmed	Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells
title_sort	trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4503967311b2473da2382379507f5f03
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Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells

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