AT1 receptor blockade attenuates insulin resistance and myocardial remodeling in rats with diet-induced obesity.

<h4>Background</h4>Although obesity has been associated with metabolic and cardiac disturbances, the carrier mechanisms for these responses are poorly understood. This study analyzed whether angiotensin II blockade attenuates metabolic and cardiovascular disorders in rats with diet-induc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Silvio A Oliveira-Junior, Paula F Martinez, Danielle M Guizoni, Dijon H S Campos, Tiago Fernandes, Edilamar M Oliveira, Marina P Okoshi, Katashi Okoshi, Carlos R Padovani, Antonio C Cicogna
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4508166f25834ed688bf41b2f2af5fe2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4508166f25834ed688bf41b2f2af5fe2
record_format	dspace
spelling	oai:doaj.org-article:4508166f25834ed688bf41b2f2af5fe22021-11-18T08:36:08ZAT1 receptor blockade attenuates insulin resistance and myocardial remodeling in rats with diet-induced obesity.1932-620310.1371/journal.pone.0086447https://doaj.org/article/4508166f25834ed688bf41b2f2af5fe22014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24466104/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Although obesity has been associated with metabolic and cardiac disturbances, the carrier mechanisms for these responses are poorly understood. This study analyzed whether angiotensin II blockade attenuates metabolic and cardiovascular disorders in rats with diet-induced obesity.<h4>Material and methods</h4>Wistar-Kyoto (n = 40) rats were subjected to control (C; 3.2 kcal/g) and hypercaloric diets (OB; 4.6 kcal/g) for 30 weeks. Subsequently, rats were distributed to four groups: C, CL, OB, and OBL. L groups received Losartan (30 mg/kg/day) for five weeks. After this period we performed in vivo glucose tolerance and insulin tolerance tests, and measured triacylglycerol, insulin, angiotensin-converting enzyme activity (ACE), and leptin levels. Cardiovascular analyzes included systolic blood pressure (SBP), echocardiography, myocardial morphometric study, myosin heavy chain composition, and measurements of myocardial protein levels of angiotensin, extracellular signal-regulated (ERK1/2), c-Jun amino-terminal kinases (JNK), insulin receptor subunit β (βIR), and phosphatidylinositol 3-kinase (PI3K) by Western Blot.<h4>Results</h4>Glucose metabolism, insulin, lipid, and ACE activity disorders observed with obesity were minimized by Losartan. Moreover, obesity was associated with increased SBP, myocardial hypertrophy, interstitial fibrosis and improved systolic performance; these effects were also minimized with Losartan. On a molecular level, OB exhibited higher ERK, Tyr-phosphorylated βIR, and PI3K expression, and reduced myocardial angiotensin and JNK expression. ERK and JNK expression were regulated in the presence of Losartan, while angiotensin, Tyr-βRI, total and Tyr-phosphorylated PI3K expression were elevated in the OBL group.<h4>Conclusion</h4>Angiotensin II blockade with Losartan attenuates obesity-induced metabolic and cardiovascular changes.Silvio A Oliveira-JuniorPaula F MartinezDanielle M GuizoniDijon H S CamposTiago FernandesEdilamar M OliveiraMarina P OkoshiKatashi OkoshiCarlos R PadovaniAntonio C CicognaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86447 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Silvio A Oliveira-Junior Paula F Martinez Danielle M Guizoni Dijon H S Campos Tiago Fernandes Edilamar M Oliveira Marina P Okoshi Katashi Okoshi Carlos R Padovani Antonio C Cicogna AT1 receptor blockade attenuates insulin resistance and myocardial remodeling in rats with diet-induced obesity.
description	<h4>Background</h4>Although obesity has been associated with metabolic and cardiac disturbances, the carrier mechanisms for these responses are poorly understood. This study analyzed whether angiotensin II blockade attenuates metabolic and cardiovascular disorders in rats with diet-induced obesity.<h4>Material and methods</h4>Wistar-Kyoto (n = 40) rats were subjected to control (C; 3.2 kcal/g) and hypercaloric diets (OB; 4.6 kcal/g) for 30 weeks. Subsequently, rats were distributed to four groups: C, CL, OB, and OBL. L groups received Losartan (30 mg/kg/day) for five weeks. After this period we performed in vivo glucose tolerance and insulin tolerance tests, and measured triacylglycerol, insulin, angiotensin-converting enzyme activity (ACE), and leptin levels. Cardiovascular analyzes included systolic blood pressure (SBP), echocardiography, myocardial morphometric study, myosin heavy chain composition, and measurements of myocardial protein levels of angiotensin, extracellular signal-regulated (ERK1/2), c-Jun amino-terminal kinases (JNK), insulin receptor subunit β (βIR), and phosphatidylinositol 3-kinase (PI3K) by Western Blot.<h4>Results</h4>Glucose metabolism, insulin, lipid, and ACE activity disorders observed with obesity were minimized by Losartan. Moreover, obesity was associated with increased SBP, myocardial hypertrophy, interstitial fibrosis and improved systolic performance; these effects were also minimized with Losartan. On a molecular level, OB exhibited higher ERK, Tyr-phosphorylated βIR, and PI3K expression, and reduced myocardial angiotensin and JNK expression. ERK and JNK expression were regulated in the presence of Losartan, while angiotensin, Tyr-βRI, total and Tyr-phosphorylated PI3K expression were elevated in the OBL group.<h4>Conclusion</h4>Angiotensin II blockade with Losartan attenuates obesity-induced metabolic and cardiovascular changes.
format	article
author	Silvio A Oliveira-Junior Paula F Martinez Danielle M Guizoni Dijon H S Campos Tiago Fernandes Edilamar M Oliveira Marina P Okoshi Katashi Okoshi Carlos R Padovani Antonio C Cicogna
author_facet	Silvio A Oliveira-Junior Paula F Martinez Danielle M Guizoni Dijon H S Campos Tiago Fernandes Edilamar M Oliveira Marina P Okoshi Katashi Okoshi Carlos R Padovani Antonio C Cicogna
author_sort	Silvio A Oliveira-Junior
title	AT1 receptor blockade attenuates insulin resistance and myocardial remodeling in rats with diet-induced obesity.
title_short	AT1 receptor blockade attenuates insulin resistance and myocardial remodeling in rats with diet-induced obesity.
title_full	AT1 receptor blockade attenuates insulin resistance and myocardial remodeling in rats with diet-induced obesity.
title_fullStr	AT1 receptor blockade attenuates insulin resistance and myocardial remodeling in rats with diet-induced obesity.
title_full_unstemmed	AT1 receptor blockade attenuates insulin resistance and myocardial remodeling in rats with diet-induced obesity.
title_sort	at1 receptor blockade attenuates insulin resistance and myocardial remodeling in rats with diet-induced obesity.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4508166f25834ed688bf41b2f2af5fe2
work_keys_str_mv	AT silvioaoliveirajunior at1receptorblockadeattenuatesinsulinresistanceandmyocardialremodelinginratswithdietinducedobesity AT paulafmartinez at1receptorblockadeattenuatesinsulinresistanceandmyocardialremodelinginratswithdietinducedobesity AT daniellemguizoni at1receptorblockadeattenuatesinsulinresistanceandmyocardialremodelinginratswithdietinducedobesity AT dijonhscampos at1receptorblockadeattenuatesinsulinresistanceandmyocardialremodelinginratswithdietinducedobesity AT tiagofernandes at1receptorblockadeattenuatesinsulinresistanceandmyocardialremodelinginratswithdietinducedobesity AT edilamarmoliveira at1receptorblockadeattenuatesinsulinresistanceandmyocardialremodelinginratswithdietinducedobesity AT marinapokoshi at1receptorblockadeattenuatesinsulinresistanceandmyocardialremodelinginratswithdietinducedobesity AT katashiokoshi at1receptorblockadeattenuatesinsulinresistanceandmyocardialremodelinginratswithdietinducedobesity AT carlosrpadovani at1receptorblockadeattenuatesinsulinresistanceandmyocardialremodelinginratswithdietinducedobesity AT antonioccicogna at1receptorblockadeattenuatesinsulinresistanceandmyocardialremodelinginratswithdietinducedobesity
_version_	1718421557085208576

AT1 receptor blockade attenuates insulin resistance and myocardial remodeling in rats with diet-induced obesity.

Ejemplares similares