Evaluation of Anthracnose Resistance in Pepper (Capsicum spp.) Genetic Resources

Anthracnose (Colletotrichum spp.), is one of the major yield losing fungal disease in both pre- and post-harvest stage of pepper (Capsicum spp.) production worldwide. Among the Colletotrichum spp., C. acutatum has strong pat...

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Autores principales:	Na-Young Ro, Raveendar Sebastin, On-Sook Hur, Gyu-Taek Cho, Bora Geum, Yong-Jik Lee, Byoung-Cheorl Kang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	anthracnose resistance <i>Capsicum</i> <i>Colletotrichum</i> spp. pepper genetic resources Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/450a08b13e1f44fcbd81ea83e9895141
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spelling	oai:doaj.org-article:450a08b13e1f44fcbd81ea83e98951412021-11-25T17:47:21ZEvaluation of Anthracnose Resistance in Pepper (<i>Capsicum</i> spp.) Genetic Resources10.3390/horticulturae71104602311-7524https://doaj.org/article/450a08b13e1f44fcbd81ea83e98951412021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/460https://doaj.org/toc/2311-7524Anthracnose (<i>Colletotrichum</i> spp.), is one of the major yield losing fungal disease in both pre- and post-harvest stage of pepper (<i>Capsicum</i> spp.) production worldwide. Among the <i>Colletotrichum</i> spp., <i>C. acutatum</i> has strong pathogenicity, which infects both immature and mature pepper fruit leads to severe economic losses in pepper production. Inheritance of anthracnose disease resistance was evaluated with 3738 pepper genetic resources which was collected from different countries and conserved at Korean genebank. The resistance analysis against pepper anthracnose (<i>C. acutatum</i>) was performed on detached mature green and red fruits under laboratory conditions by spray (non-wounding) and microinjection (wounding) inoculation methods. In the primary screening, about 261 accessions were appeared to be resistant against <i>C. acutatum</i> in spray inoculation. The resistant accessions were further evaluated with microinjection (wounding) inoculation method using the fungal (<i>C. acutatum</i>) isolate of pepper anthracnose. There were highly significant differences in the disease severity and distribution of disease rating scale, considering all the sources has significant genetic variation. Finally, the anthracnose resistant pepper accessions have been validated with cleaved amplified polymorphic sequence (CAPS) and high-resolution melting (HRM) markers in which, the CAPS and HRM marker analysis showed four types of genotypes such as resistant (R), susceptible (S), heterozygous (H) and Unidentified type (UT) or not detection. The <i>Capsicum</i> accessions showing high level of resistance to the pathogen could be used as source material in breeding programs for resistance to anthracnose disease.Na-Young RoRaveendar SebastinOn-Sook HurGyu-Taek ChoBora GeumYong-Jik LeeByoung-Cheorl KangMDPI AGarticleanthracnose resistance<i>Capsicum</i><i>Colletotrichum</i> spp.pepper genetic resourcesPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 460, p 460 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anthracnose resistance <i>Capsicum</i> <i>Colletotrichum</i> spp. pepper genetic resources Plant culture SB1-1110
spellingShingle	anthracnose resistance <i>Capsicum</i> <i>Colletotrichum</i> spp. pepper genetic resources Plant culture SB1-1110 Na-Young Ro Raveendar Sebastin On-Sook Hur Gyu-Taek Cho Bora Geum Yong-Jik Lee Byoung-Cheorl Kang Evaluation of Anthracnose Resistance in Pepper (<i>Capsicum</i> spp.) Genetic Resources
description	Anthracnose (<i>Colletotrichum</i> spp.), is one of the major yield losing fungal disease in both pre- and post-harvest stage of pepper (<i>Capsicum</i> spp.) production worldwide. Among the <i>Colletotrichum</i> spp., <i>C. acutatum</i> has strong pathogenicity, which infects both immature and mature pepper fruit leads to severe economic losses in pepper production. Inheritance of anthracnose disease resistance was evaluated with 3738 pepper genetic resources which was collected from different countries and conserved at Korean genebank. The resistance analysis against pepper anthracnose (<i>C. acutatum</i>) was performed on detached mature green and red fruits under laboratory conditions by spray (non-wounding) and microinjection (wounding) inoculation methods. In the primary screening, about 261 accessions were appeared to be resistant against <i>C. acutatum</i> in spray inoculation. The resistant accessions were further evaluated with microinjection (wounding) inoculation method using the fungal (<i>C. acutatum</i>) isolate of pepper anthracnose. There were highly significant differences in the disease severity and distribution of disease rating scale, considering all the sources has significant genetic variation. Finally, the anthracnose resistant pepper accessions have been validated with cleaved amplified polymorphic sequence (CAPS) and high-resolution melting (HRM) markers in which, the CAPS and HRM marker analysis showed four types of genotypes such as resistant (R), susceptible (S), heterozygous (H) and Unidentified type (UT) or not detection. The <i>Capsicum</i> accessions showing high level of resistance to the pathogen could be used as source material in breeding programs for resistance to anthracnose disease.
format	article
author	Na-Young Ro Raveendar Sebastin On-Sook Hur Gyu-Taek Cho Bora Geum Yong-Jik Lee Byoung-Cheorl Kang
author_facet	Na-Young Ro Raveendar Sebastin On-Sook Hur Gyu-Taek Cho Bora Geum Yong-Jik Lee Byoung-Cheorl Kang
author_sort	Na-Young Ro
title	Evaluation of Anthracnose Resistance in Pepper (<i>Capsicum</i> spp.) Genetic Resources
title_short	Evaluation of Anthracnose Resistance in Pepper (<i>Capsicum</i> spp.) Genetic Resources
title_full	Evaluation of Anthracnose Resistance in Pepper (<i>Capsicum</i> spp.) Genetic Resources
title_fullStr	Evaluation of Anthracnose Resistance in Pepper (<i>Capsicum</i> spp.) Genetic Resources
title_full_unstemmed	Evaluation of Anthracnose Resistance in Pepper (<i>Capsicum</i> spp.) Genetic Resources
title_sort	evaluation of anthracnose resistance in pepper (<i>capsicum</i> spp.) genetic resources
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/450a08b13e1f44fcbd81ea83e9895141
work_keys_str_mv	AT nayoungro evaluationofanthracnoseresistanceinpeppericapsicumisppgeneticresources AT raveendarsebastin evaluationofanthracnoseresistanceinpeppericapsicumisppgeneticresources AT onsookhur evaluationofanthracnoseresistanceinpeppericapsicumisppgeneticresources AT gyutaekcho evaluationofanthracnoseresistanceinpeppericapsicumisppgeneticresources AT borageum evaluationofanthracnoseresistanceinpeppericapsicumisppgeneticresources AT yongjiklee evaluationofanthracnoseresistanceinpeppericapsicumisppgeneticresources AT byoungcheorlkang evaluationofanthracnoseresistanceinpeppericapsicumisppgeneticresources
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Evaluation of Anthracnose Resistance in Pepper (<i>Capsicum</i> spp.) Genetic Resources

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