Bacteriological and mechanical impact of the Sterrad sterilization method on personalized 3D printed guides for mandibular reconstruction

Abstract Surgical cutting guides are increasingly used for maxillofacial reconstruction. They are usually provided by laboratories. In recent years, surgical teams have published studies on the possibility of manufacturing their own cutting guides thanks to 3D printers. The object of this study is t...

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Autores principales:	Romain Bosc, Lionel Tortolano, Barbara Hersant, Moussa Oudjhani, Céline Leplay, Paul L. Woerther, Paola Aguilar, Ronan Leguen, Jean-Paul Meningaud
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4510200a38f24da3aabf2241dfbd43c1
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spelling	oai:doaj.org-article:4510200a38f24da3aabf2241dfbd43c12021-12-02T14:01:22ZBacteriological and mechanical impact of the Sterrad sterilization method on personalized 3D printed guides for mandibular reconstruction10.1038/s41598-020-79752-72045-2322https://doaj.org/article/4510200a38f24da3aabf2241dfbd43c12021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79752-7https://doaj.org/toc/2045-2322Abstract Surgical cutting guides are increasingly used for maxillofacial reconstruction. They are usually provided by laboratories. In recent years, surgical teams have published studies on the possibility of manufacturing their own cutting guides thanks to 3D printers. The object of this study is to analyze the impact of the sterilization on the surface of those personalized models and to assess the effectiveness of sterilization. Using the data from high-resolution CT scan of patient, 3D models were generated through computerized assisted design and fabricated with a 3D printer using Acrylonitrile Butadiene Styrene (ABS). For the sterilization, a Sterrad method was used. In order to evaluate the effectiveness of sterilization, 3D models were artificially contaminated with several bacterial reference strains, sterilized and finally cultured. The surfaces and mechanical modifications were analyzed before and after sterilization with infrared spectrometry, surface contact angle, extensometer, scanning electron microscopy and atomic force microscopy. Ten models of different shapes and 24 samples were fabricated, sterilized and analyzed. The 3D models were designed in 48 h, printed in an average of 122 min and underwent a 47 min cycle of sterilization. All experimentally contaminated 3D models were negative in culture, with at least, a six log reduction of the initial inoculum. The hydrophobicity and roughness of the surface suffered few changes. The reproducibility of this procedure was proved by identical results in the three sterilization rounds. Using Sterrad process for the sterilization of ABS printed material doesn’t represent a bacterial risk for the patient. It is a feasible and safe innovative reconstructive method that can save time particularly for oncological cases.Romain BoscLionel TortolanoBarbara HersantMoussa OudjhaniCéline LeplayPaul L. WoertherPaola AguilarRonan LeguenJean-Paul MeningaudNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Romain Bosc Lionel Tortolano Barbara Hersant Moussa Oudjhani Céline Leplay Paul L. Woerther Paola Aguilar Ronan Leguen Jean-Paul Meningaud Bacteriological and mechanical impact of the Sterrad sterilization method on personalized 3D printed guides for mandibular reconstruction
description	Abstract Surgical cutting guides are increasingly used for maxillofacial reconstruction. They are usually provided by laboratories. In recent years, surgical teams have published studies on the possibility of manufacturing their own cutting guides thanks to 3D printers. The object of this study is to analyze the impact of the sterilization on the surface of those personalized models and to assess the effectiveness of sterilization. Using the data from high-resolution CT scan of patient, 3D models were generated through computerized assisted design and fabricated with a 3D printer using Acrylonitrile Butadiene Styrene (ABS). For the sterilization, a Sterrad method was used. In order to evaluate the effectiveness of sterilization, 3D models were artificially contaminated with several bacterial reference strains, sterilized and finally cultured. The surfaces and mechanical modifications were analyzed before and after sterilization with infrared spectrometry, surface contact angle, extensometer, scanning electron microscopy and atomic force microscopy. Ten models of different shapes and 24 samples were fabricated, sterilized and analyzed. The 3D models were designed in 48 h, printed in an average of 122 min and underwent a 47 min cycle of sterilization. All experimentally contaminated 3D models were negative in culture, with at least, a six log reduction of the initial inoculum. The hydrophobicity and roughness of the surface suffered few changes. The reproducibility of this procedure was proved by identical results in the three sterilization rounds. Using Sterrad process for the sterilization of ABS printed material doesn’t represent a bacterial risk for the patient. It is a feasible and safe innovative reconstructive method that can save time particularly for oncological cases.
format	article
author	Romain Bosc Lionel Tortolano Barbara Hersant Moussa Oudjhani Céline Leplay Paul L. Woerther Paola Aguilar Ronan Leguen Jean-Paul Meningaud
author_facet	Romain Bosc Lionel Tortolano Barbara Hersant Moussa Oudjhani Céline Leplay Paul L. Woerther Paola Aguilar Ronan Leguen Jean-Paul Meningaud
author_sort	Romain Bosc
title	Bacteriological and mechanical impact of the Sterrad sterilization method on personalized 3D printed guides for mandibular reconstruction
title_short	Bacteriological and mechanical impact of the Sterrad sterilization method on personalized 3D printed guides for mandibular reconstruction
title_full	Bacteriological and mechanical impact of the Sterrad sterilization method on personalized 3D printed guides for mandibular reconstruction
title_fullStr	Bacteriological and mechanical impact of the Sterrad sterilization method on personalized 3D printed guides for mandibular reconstruction
title_full_unstemmed	Bacteriological and mechanical impact of the Sterrad sterilization method on personalized 3D printed guides for mandibular reconstruction
title_sort	bacteriological and mechanical impact of the sterrad sterilization method on personalized 3d printed guides for mandibular reconstruction
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4510200a38f24da3aabf2241dfbd43c1
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Bacteriological and mechanical impact of the Sterrad sterilization method on personalized 3D printed guides for mandibular reconstruction

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