Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon

N Ashwanikumar,1,* Nisha Asok Kumar,2,* S Asha Nair,2 GS Vinod Kumar11Chemical Biology, 2Cancer Research Programme, Rajiv Gandhi Center for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India *These authors contributed equally to this workAbstract: Methacrylic-based copolymers in drug-deliv...

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Autores principales:	Ashwanikumar N, Kumar NA, Nair SA, Kumar GS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4513627e80e046f2bf64b1a66d35febb
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spelling	oai:doaj.org-article:4513627e80e046f2bf64b1a66d35febb2021-12-02T01:46:38ZMethacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon1176-91141178-2013https://doaj.org/article/4513627e80e046f2bf64b1a66d35febb2012-11-01T00:00:00Zhttp://www.dovepress.com/methacrylic-based-nanogels-for-the-ph-sensitive-delivery-of-5-fluorour-a11522https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013N Ashwanikumar,1,* Nisha Asok Kumar,2,* S Asha Nair,2 GS Vinod Kumar11Chemical Biology, 2Cancer Research Programme, Rajiv Gandhi Center for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India *These authors contributed equally to this workAbstract: Methacrylic-based copolymers in drug-delivery systems demonstrate a pH-sensitive drug-releasing behavior in the colon. In this study, copolymers of methacrylic acid and 2-ethyl hexyl acrylate were prepared using a microemulsion polymerization technique. The purified copolymer was characterized by Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and differential scanning calorimetry. 5-Fluorouracil (5-FU) was entrapped within methacrylic-based copolymers by a solvent evaporation method. The size of the nanogels formed was characterized by transmission electron microscopy and atomic force microscopy. In vitro drug-release studies using phosphate-buffered saline at different pH levels demonstrated the sustained release of 5-FU and its pH dependence. Cell proliferation assay of a human colon tumor colon cancer cell line (HCT-116) was performed and showed that the nanogels containing 5-FU exhibited considerable cytotoxicity in comparison with free 5-FU. Cell uptake of the nanogels was also monitored using confocal microscopy. Western blot analysis and flow cytometry studies confirmed that the nanogels could be successfully used as an efficient vector for pH-sensitive and controlled delivery of drugs specifically targeted to the colon.Keywords: 5-FU, methacrylic polymer, colon cancer, nanogelAshwanikumar NKumar NANair SAKumar GSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5769-5779 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ashwanikumar N Kumar NA Nair SA Kumar GS Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon
description	N Ashwanikumar,1,* Nisha Asok Kumar,2,* S Asha Nair,2 GS Vinod Kumar11Chemical Biology, 2Cancer Research Programme, Rajiv Gandhi Center for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India *These authors contributed equally to this workAbstract: Methacrylic-based copolymers in drug-delivery systems demonstrate a pH-sensitive drug-releasing behavior in the colon. In this study, copolymers of methacrylic acid and 2-ethyl hexyl acrylate were prepared using a microemulsion polymerization technique. The purified copolymer was characterized by Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and differential scanning calorimetry. 5-Fluorouracil (5-FU) was entrapped within methacrylic-based copolymers by a solvent evaporation method. The size of the nanogels formed was characterized by transmission electron microscopy and atomic force microscopy. In vitro drug-release studies using phosphate-buffered saline at different pH levels demonstrated the sustained release of 5-FU and its pH dependence. Cell proliferation assay of a human colon tumor colon cancer cell line (HCT-116) was performed and showed that the nanogels containing 5-FU exhibited considerable cytotoxicity in comparison with free 5-FU. Cell uptake of the nanogels was also monitored using confocal microscopy. Western blot analysis and flow cytometry studies confirmed that the nanogels could be successfully used as an efficient vector for pH-sensitive and controlled delivery of drugs specifically targeted to the colon.Keywords: 5-FU, methacrylic polymer, colon cancer, nanogel
format	article
author	Ashwanikumar N Kumar NA Nair SA Kumar GS
author_facet	Ashwanikumar N Kumar NA Nair SA Kumar GS
author_sort	Ashwanikumar N
title	Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon
title_short	Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon
title_full	Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon
title_fullStr	Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon
title_full_unstemmed	Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon
title_sort	methacrylic-based nanogels for the ph-sensitive delivery of 5-fluorouracil in the colon
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/4513627e80e046f2bf64b1a66d35febb
work_keys_str_mv	AT ashwanikumarn methacrylicbasednanogelsforthephsensitivedeliveryof5fluorouracilinthecolon AT kumarna methacrylicbasednanogelsforthephsensitivedeliveryof5fluorouracilinthecolon AT nairsa methacrylicbasednanogelsforthephsensitivedeliveryof5fluorouracilinthecolon AT kumargs methacrylicbasednanogelsforthephsensitivedeliveryof5fluorouracilinthecolon
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Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon

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